Process for extracting ginkgo biloba flavone, ginkgo biloba lactone and ginkgo biloba phenolic acid from ginkgo biloba leaves

Abstract

The present invention discloses a process for extracting ginkgo biloba flavone, ginkgo biloba lactone and ginkgo biloba phenolic acid from ginkgo biloba leaves. The process comprises the following steps: (1) carrying out a pretreatment on ginkgo biloba leaves; (2) carrying out extraction by an ultrasonic device; (3) carrying out reduced pressure concentration; (4) loading the resulting solution on a macroporous resin to carry out a treatment; (5) collecting the resulting eluate; (6) carrying out reduced pressure concentration; (7) detecting the resulting extract; and (8) carrying out drying and storing on the extract. With the process of the present invention, the ginkgo biloba flavone content and the ginkgo biloba lactone content in the extract prepared by the process of the present invention are high, the loss of active ingredients are avoided, the production process is simplified, the production cycle is shortened, and the ginkgo biloba phenolic acid content after treatment is substantially reduced.

Description

technical field

[0001] The invention relates to a process for extracting from ginkgo leaves, in particular to a process for extracting ginkgo flavonoids, ginkgolides and ginkgolic acids from ginkgo leaves. Background technique

[0002] Ginkgo biloba (Ginkgo biloba) Ginkgo biloba ) is a unique tree species in my country. Ginkgo biloba contains active ingredients such as flavonoids (about 20 species), terpenes, phenols, beneficial trace elements and 17 amino acids. At present, Ginkgo biloba extract has been developed into medicines, health care products and cosmetics in the United States, Japan, Germany, Italy, Korea and other countries. my country has also set off an upsurge in the development and research of Ginkgo biloba. There are already hundreds of manufacturers producing ginkgo dry extract in China, and ginkgo leaf products have been put into the market one after another.

[0003] Ginkgo biloba extract contains a large amount of ginkgo flavonoids and ginkgolides, whic...

Examples

Embodiment 1

[0019] (1) Pre-treatment of ginkgo leaves: dry the dried ginkgo leaves and pulverize them into powder;

[0020] (2) Put the raw material and water into the extraction tank at a ratio of 1:10, and use ultrasonic to extract. The ultrasonic working frequency of ultrasonic extraction is 70KHz, and the extraction time is 25 minutes. Filter, add water to the original volume, and continue ultrasonic extraction for 25 minutes. , filter, and combine the filtrate;

[0021] (3) Concentration under reduced pressure: put the extract into a rotary evaporator to concentrate under reduced pressure, control the temperature at 55°C, control the vacuum degree at 0.1MPa, and concentrate the volume to 1 / 10 of the original volume;

[0022] (4) Enter the macroporous resin for treatment: after filtering the concentrated extract in step (3), directly enter the macroporous resin for treatment;

[0023] (5) Collect the eluate: collect the effluent from the macroporous resin column and the eluate eluted...

Embodiment 2

[0028] (1) Pre-treatment of ginkgo leaves: dry the dried ginkgo leaves and crush them into powder;

[0029] (2) Ultrasonic extraction, put the raw material and methanol with a volume concentration of 75% into the extraction tank at a ratio of 1:10, and use ultrasonic waves for extraction. The ultrasonic working frequency of ultrasonic extraction is 70KHz, and the extraction time is 25 minutes at 25°C. Filter , add methanol to the original volume, continue ultrasonic extraction for 25 minutes, filter, and combine the filtrates;

[0030] (3) Concentration under reduced pressure: put the extract into a rotary evaporator to concentrate under reduced pressure, control the temperature at 45°C, control the vacuum degree at 0.1MPa, and concentrate the volume to 1 / 8 of the original volume;

[0031] (4) Enter the macroporous resin for treatment: after filtering the concentrated extract in step (3), directly enter the macroporous resin for treatment;

[0032] (5) Collect the eluate: col...

technical field

[0001] The invention relates to a process for extracting from ginkgo leaves, in particular to a process for extracting ginkgo flavonoids, ginkgolides and ginkgolic acids from ginkgo leaves. Background technique

[0002] Ginkgo biloba (Ginkgo biloba) Ginkgo biloba ) is a unique tree species in my country. Ginkgo biloba contains active ingredients such as flavonoids (about 20 species), terpenes, phenols, beneficial trace elements and 17 amino acids. At present, Ginkgo biloba extract has been developed into medicines, health care products and cosmetics in the United States, Japan, Germany, Italy, Korea and other countries. my country has also set off an upsurge in the development and research of Ginkgo biloba. There are already hundreds of manufacturers producing ginkgo dry extract in China, and ginkgo leaf products have been put into the market one after another.

[0003] Ginkgo biloba extract contains a large amount of ginkgo flavonoids and ginkgolides, whic...

Examples

Embodiment 1

[0019] (1) Pre-treatment of ginkgo leaves: dry the dried ginkgo leaves and pulverize them into powder;

[0020] (2) Put the raw material and water into the extraction tank at a ratio of 1:10, and use ultrasonic to extract. The ultrasonic working frequency of ultrasonic extraction is 70KHz, and the extraction time is 25 minutes. Filter, add water to the original volume, and continue ultrasonic extraction for 25 minutes. , filter, and combine the filtrate;

[0021] (3) Concentration under reduced pressure: put the extract into a rotary evaporator to concentrate under reduced pressure, control the temperature at 55°C, control the vacuum degree at 0.1MPa, and concentrate the volume to 1 / 10 of the original volume;

[0022] (4) Enter the macroporous resin for treatment: after filtering the concentrated extract in step (3), directly enter the macroporous resin for treatment;

[0023] (5) Collect the eluate: collect the effluent from the macroporous resin column and the eluate eluted...

Embodiment 2

[0028] (1) Pre-treatment of ginkgo leaves: dry the dried ginkgo leaves and crush them into powder;

[0029] (2) Ultrasonic extraction, put the raw material and methanol with a volume concentration of 75% into the extraction tank at a ratio of 1:10, and use ultrasonic waves for extraction. The ultrasonic working frequency of ultrasonic extraction is 70KHz, and the extraction time is 25 minutes at 25°C. Filter , add methanol to the original volume, continue ultrasonic extraction for 25 minutes, filter, and combine the filtrates;

[0030] (3) Concentration under reduced pressure: put the extract into a rotary evaporator to concentrate under reduced pressure, control the temperature at 45°C, control the vacuum degree at 0.1MPa, and concentrate the volume to 1 / 8 of the original volume;

[0031] (4) Enter the macroporous resin for treatment: after filtering the concentrated extract in step (3), directly enter the macroporous resin for treatment;

[0032] (5) Collect the eluate: col...