41 results about "Bilobol" patented technology

The comprehensive gingko outer seed coat utilizing process belongs to the field of gingko processing technology. Of the fresh gingko outer seed coat as material, the components capable of being fermented are water extracted as the fermentation culture medium and is fermented and extracted to produce alcohol, and the residue after water extraction is leached with 80 % concentration alcohol solution as the leaching solvent. The leached liquid is vacuum concentrated, separated and purified with macroporous resin LSA-21 and silica gel column successively to obtain gingko phenonic acid and similar matter in purity over 90 %. The present invention is significant in utilizing gingko resource and protecting ecologic environment.

The invention discloses lucid ganoderma-gingko wine, relating to the technical field of biological engineering. According to the wine provided by the invention, the alcoholic strength is 8-16%, the content of general flavone is 1-5 mg / L, the content of gingko lactone is 1-6 mg / L, the content of general terpenes compound is 30-800 mg / L, the content of protein is 60-200 mg / L, the content of ganoderic acid is 10-17 mg / L, the content of soluble sugar is 0-2.0%, the content of gingko phenolic acid is 0-5 ppm and the content of hydrocyanic acid is 0-0.1 microgram / liter. According to the preparationmethod provided by the invention, the gingko is hydrolyzed by utilizing a double-enzyme method to obtain a gingko hydrolyzing solution and the gingko hydrolyzing solution contains active compounds including gingko flavone, terpenes compounds, amino acid, peptides and starch and the like, wherein the content of the amino acid and the peptides is not less than 1% and the content of the starch is not less than 2%; the hydrolyzing solution is converted through lucid ganoderma strains and a rice mold culture is added to ferment to obtain fermentation liquor; the fermentation liquor is filtered andblended to obtain the lucid ganoderma-gingko wine. The wine provided by the invention contains the active components of the gingko and the lucid ganoderma and has the functions of preventing hypertension, resisting oxidization and the like.

The present invention discloses a process for extracting ginkgo biloba flavone, ginkgo biloba lactone and ginkgo biloba phenolic acid from ginkgo biloba leaves. The process comprises the following steps: (1) carrying out a pretreatment on ginkgo biloba leaves; (2) carrying out extraction by an ultrasonic device; (3) carrying out reduced pressure concentration; (4) loading the resulting solution on a macroporous resin to carry out a treatment; (5) collecting the resulting eluate; (6) carrying out reduced pressure concentration; (7) detecting the resulting extract; and (8) carrying out drying and storing on the extract. With the process of the present invention, the ginkgo biloba flavone content and the ginkgo biloba lactone content in the extract prepared by the process of the present invention are high, the loss of active ingredients are avoided, the production process is simplified, the production cycle is shortened, and the ginkgo biloba phenolic acid content after treatment is substantially reduced.

The invention relates to a method for extracting gingko powder from gingkoes and preparing gingko healthy wine, comprising the following steps of: 1, selecting high-quality gingko fruit (gingkoes); 2, husking the fruit and removing red skins and fruit cores in a sterile room; 3, taking fruit flesh and pulverizing the fruit flesh into fine paste shape fruit flesh paste; and 4, pouring the fruit flesh paste into 50% of white spirit (the proportion of the paste to the white spirit is 1 to 5), uniformly stirring the mixture, separating the white spirit and a white sediment by using a filtration method after the mixture is deposited for 8 to 10 days, and obtaining gingko fruit wine and the white sediment and drying the white sediment to obtain gingko phenol. The proportion of distiller yeast to glutinous rice to gingko leaves to water in a formulation is 1 to 8 to 2 to 19. The operation method comprises the following steps: washing gingko leaves, drying the gingko leaves, boiling the leaves with water for 15 minutes, squeezing out leaf juice, boiling the leaf juice and the glutinous rice after being washed into porridge, cooling the porridge to 25 DEG C, adding the distiller yeast into the porridge, pouring the mixture into a wine crock, brewing the gingko leaf wine after 10 to 15 days, and obtaining the gingko leaf wine by filtration; blending the gingko fruit wine and the ginkgo leaf wine according to the need to obtain the gingko healthy wine. The gingko healthy wine has abundant nutrition, has a natural health-care function and can resist and cure diseases, delay consenescence and benefit longevity after being drunk for a long time.

The invention relates to application of gingkolic acid in preparation of an external preparation for treating osmidrosis. The gingkolic acid comprises ginkgoic acid, ginkgol, ginkgo diphenol and the like, and can be extracted from leaf, fruit, external exotesta, rhizome and other parts of ginkgo through a chemical separation method. The osmidrosis includes the osmidrosis of axillary part, foot part, perineum and other parts. The drug, cosmetic or disinfectant external preparation containing gingkolic acid for treating osmidrosis contains 0.001-80wt% of gingkolic acid and a pharmaceutically common external preparation matrix or acceptable carrier; and the preparation is any one pharmaceutically external dosage form. Pharmacological experiment researches show that the external preparation has the effects of inhibiting the growth and propagation of bacteria on skin surface and permeating into skin to destroy large sudoriferous gland, and inhibiting the generation of free fatty acid, ammonia nitrogen compound and other smelling substances in sweat. Clinical trial shows that the external preparation has significant treatment effect on the osmidrosis, has effective rate up to 100%, is safe to use and has no side effect.

The invention discloses a method for extracting ginkgo flavonoids from ginkgo leaves. The method comprises the steps: (1) pre-treating the ginkgo leaves; (2) performing extraction with an ultrasonic device; (3) performing concentration under reduced pressure; (4) performing treatment through macroporous resin; (5) collecting an eluate; (6) performing concentration under reduced pressure; (7) detecting an extract; and (8) performing drying and storage on the extract obtained through the steps. The obtained extract has high content of ginkgo flavonoids and ginkgolides, loss of active ingredientscan be avoided, the production process is simplified, the production cycle can be shortened, and meanwhile, the content of ginkgolic acids obtained after treatment is reduced greatly.

The invention discloses a method for reducing the content of phenolic acid in a Folium Ginkgo extract, and belongs to the medicine processing field. The method comprises the following steps: drying and crushing Folium Ginkgo, sieving by a 80 mesh sieve, carrying out acoustomagnetic radiation extraction of the obtained processed Folium Ginkgo in water (added with sodium carbonate or citric acid for adjusting the pH value), mixing obtained extract liquids, concentrating, filtering, allowing the obtained filtrate to go through a macro-porous resin, concentrating the obtained alcohol phase, and detecting the content of phenolic acid in the extract through using an HPLC-UV process. The method has the advantages of energy saving, small production space, and reduction of the content of gingkolic acid in the Folium Ginkgo extract.

The present invention discloses a new ginkgodamole composition, medicine composition prepared hy using it, in particular, injection and their preparation method. Said ginkgodamole composition mainly contains the following components: (by wt%) 18-65% of ginkgo total flavone glycoside, 6-32% of dipyridamole, 4-25% of ginkgo total lactone and the heavy metal content is less than 9.4 ppm (weight), inwhich the ginkgo total lactone is formed from bilobalide, ginkgolide A, ginkgolide B and ginkgolide C according to the weight ratio of 4-40:7-40:3-30:3-40. Said medicine preparation, specially injection have obvious therapeutic effect for curing angiocardiopathy and cerebrovascular diseases.

The invention relates to application of ginkgol C17:1 in treatment of liver cancer, belonging to the field of biological drugs. The ginkgol C17:1 is prepared through a method comprising the following steps: preparing ginkgolic acid from gingko (leaves, fruits and episperm); performing heat treatment to decarboxylate the ginkgolic acid; and separating, and purifying to obtain the ginkgol C17:1, wherein structural identification shows that the ginkgol C17:1 has cis / trans isomeric structures. The prepared ginkgol C17:1 can effectively inhibit proliferation of human liver cancer cells SMMC-7721 and inhibit migration of the SMMC-7721 cells in vitro, and drug resistance can not be easily produced; and the prepared ginkgol C17:1 can inhibit growth of nude mouse loaded HepG solid tumors in vivo. The new application of the ginkgol C17:1 provided by the invention is of great importance in preparation of antitumor drugs and especially in preparation of anti-liver cancer drugs.

The invention relates to application of gingkolic acid in preparation of an external preparation for treating osmidrosis. The gingkolic acid comprises ginkgoic acid, ginkgol, ginkgo diphenol and the like, and can be extracted from leaf, fruit, external exotesta, rhizome and other parts of ginkgo through a chemical separation method. The osmidrosis includes the osmidrosis of axillary part, foot part, perineum and other parts. The drug, cosmetic or disinfectant external preparation containing gingkolic acid for treating osmidrosis contains 0.001-80wt% of gingkolic acid and a pharmaceutically common external preparation matrix or acceptable carrier; and the preparation is any one pharmaceutically external dosage form. Pharmacological experiment researches show that the external preparation has the effects of inhibiting the growth and propagation of bacteria on skin surface and permeating into skin to destroy large sudoriferous gland, and inhibiting the generation of free fatty acid, ammonia nitrogen compound and other smelling substances in sweat. Clinical trial shows that the external preparation has significant treatment effect on the osmidrosis, has effective rate up to 100%, is safe to use and has no side effect.

The invention discloses a whole ginkgo seed powder preparation method capable of reducing the content of bilobol and ginkgolic acid, and relates to the technical field of production methods achieving deep processing of ginkgo seeds. The method comprises the process steps of conducting hulling, rinsing and leaching on selected ginkgo seeds, smashing the ginkgo seeds for pulping, adding alkali for pulp conditioning and standing, conducting infrared drying and obtaining whole ginkgo seed powder. The prepared whole ginkgo seed powder has the advantage of being low in content of ginkgolic acid and bilobol, wherein the content of bilobol and the content of ginkgolic acid are reduced by 86%-83.6% and 60.5%-38.7% respectively compared with whole ginkgo seed powder prepared by being directly dried at 60 DEG C; the obtained product is good in rehydration characteristic, and the flavor and taste of the obtained product are closer to those of fresh ginkgo seeds.

The invention discloses a novel ginkgo dipyridamole composition and a preparation method of a preparation thereof. The ginkgo dipyridamole composition mainly comprises the following ingredients by weight: 25-35% of ginkgo total flavonoid glycol glycosides, 10-15% of dipyridamole and 10-18% of ginkgo total lactones, wherein the ginkgo total lactones are composed of bilobalide, ginkgolides A, ginkgolides B, ginkgolides C and ginkgolides M according to the weight ratio of 22-30: 15-18: 5-8: 8-22: 4-8. The ginkgo dipyridamole composition is high in active ingredient content and low in volume of contained impurities of ginkgo biloba phenolic substances (such as ginkgolic acid), proteins, tannins, pyrogen materials and the like. The pharmaceutic preparation, especially injection prepared by the ginkgo dipyridamole composition is suitable for preventing and treating coronary diseases, thromboembolic diseases and the like, has obvious curative effects, and is safe in clinic use.

The invention discloses a method for solid-state fermentation of Ginkgo powder by S. coronis and the product and application thereof. The method comprises: (1) preparing a spore suspension of S. coronis; (2) preparing ginkgo powder, adding Inorganic salts, adjust the pH to prepare a solid-state fermentation medium; (3) inoculate the spore suspension of S. coronoidis in the solid-state fermentation medium, and add sterile deionized water to adjust the initial water content, cultivate and add no Bacterial deionized water, after fermentation, the solid-state fermented ginkgo powder of Saccharomyces coronoidis is obtained. The present invention uses ginkgo powder as the base material for the solid-state fermentation of S. coronoidis. After fermentation, most of the nutritional components and functional components of the ginkgo powder are retained, and the ginkgophenolic acid and MPN in the ginkgo powder are effectively degraded. Due to the growth and metabolism of the fungus, the fermented ginkgo powder contains a large number of spores of the fungus, lovastatin, polysaccharide, α-amylase, protease and many other functional health ingredients, and the flavor is obviously improved.

The invention discloses lucid ganoderma-gingko wine, relating to the technical field of biological engineering. According to the wine provided by the invention, the alcoholic strength is 8-16%, the content of general flavone is 1-5 mg / L, the content of gingko lactone is 1-6 mg / L, the content of general terpenes compound is 30-800 mg / L, the content of protein is 60-200 mg / L, the content of ganoderic acid is 10-17 mg / L, the content of soluble sugar is 0-2.0%, the content of gingko phenolic acid is 0-5 ppm and the content of hydrocyanic acid is 0-0.1 microgram / liter. According to the preparationmethod provided by the invention, the gingko is hydrolyzed by utilizing a double-enzyme method to obtain a gingko hydrolyzing solution and the gingko hydrolyzing solution contains active compounds including gingko flavone, terpenes compounds, amino acid, peptides and starch and the like, wherein the content of the amino acid and the peptides is not less than 1% and the content of the starch is not less than 2%; the hydrolyzing solution is converted through lucid ganoderma strains and a rice mold culture is added to ferment to obtain fermentation liquor; the fermentation liquor is filtered andblended to obtain the lucid ganoderma-gingko wine. The wine provided by the invention contains the active components of the gingko and the lucid ganoderma and has the functions of preventing hypertension, resisting oxidization and the like.

The invention discloses a method for liquid state fermentation of Saccharomyces coronis and the product and application thereof. The method comprises: activating and separating Saccharomyces coronis, and preparing spore suspension; making ginkgo fruit into ginkgo nuts , adding water to grind, adding inorganic salts to prepare a liquid fermentation medium; inoculating the spore suspension of Cystis coronis in the liquid fermentation medium, and vibrating and culturing to obtain a liquid fermentation product of Cystis coronis. In the present invention, ginkgo is used as a liquid fermentation medium for the coronoid fungus, and after fermentation, most of the nutritional components and functional components of the ginkgo are retained, and the contents of ginkgolic acid and MPN are reduced. In addition, after fermentation, it contains a large number of spores of Cystis coronis, lovastatin, polysaccharides, α-amylase, protease and many other functional health ingredients, and the flavor is obviously improved. Its ginkgo fermented products can be used to prepare ginkgo products such as enzymes and beverages with the functions of regulating blood lipid, regulating immunity, anti-oxidation and anti-tumor.

The invention discloses a hydrophobic deep eutectic solvent used for liquid-liquid microextraction to remove ginkgolic acids in ginkgo biloba crude extract, a preparation method and a removal method thereof, wherein the hydrophobic deep eutectic solvent consists of menthol, hexanol and Lauric acid is prepared at a molar ratio of 1:0.9:0.1 to 1:0.4:0.6. The preparation of the hydrophobic deep eutectic solvent of the present invention is simple and fast; the hydrogen bond is stable, the fluidity is good, it is a green solvent, and has the characteristics of easy biodegradation, no pollution to the environment, and high biocompatibility; the hydrophobic deep eutectic solvent prepared by the present invention The method of liquid-liquid microextraction to remove ginkgolic acids in the crude extract of ginkgo leaves can reduce the content of ginkgolic acids in the crude extract of ginkgo leaves to less than 5ppm, while retaining the content of ginkgo flavonoids and terpenes in the crude extract of ginkgo leaves The content of functional components such as esters, the whole liquid-liquid micro-extraction removal method is simple, fast, safe, high removal efficiency, low cost, and will not cause harm to the environment and human body.

The invention discloses a ginkgolic acid-rhamnolipid compound bactericide, a preparation method and application thereof, and belongs to the technical field of pesticides. The invention is prepared from the following raw materials in parts by weight: 15-25 parts of ginkgolic acid extract, 10-15 parts of rhamnolipid, 3-5 parts of synergist, 1-3 parts of wetting agent, and dispersant 1-3 parts, 0.5-1 parts of preservatives, 0.5-0.8 parts of antifreeze and 10-15 parts of fillers. The invention adopts the combination of membrane technology and macroporous adsorption resin to separate and purify the ginkgolic acid, and realizes the high-efficiency extraction of the ginkgolic acid. The present invention adds houttuynia cordata and bamboo vinegar liquid as synergists, and the two cooperate with each other to promote the fungicide of the present invention to exert the bactericidal effect, and meanwhile, the bamboo vinegar also has the effect of promoting the germination, rooting and growth of crop seeds and improving crop yield. The invention selects discarded ginkgo leaf waste residue and outer testa as raw materials to prepare the fungicide, which reduces the use of chemical pesticides and reduces environmental pollution.