41 results about "Bilobol" patented technology

The comprehensive gingko outer seed coat utilizing process belongs to the field of gingko processing technology. Of the fresh gingko outer seed coat as material, the components capable of being fermented are water extracted as the fermentation culture medium and is fermented and extracted to produce alcohol, and the residue after water extraction is leached with 80 % concentration alcohol solution as the leaching solvent. The leached liquid is vacuum concentrated, separated and purified with macroporous resin LSA-21 and silica gel column successively to obtain gingko phenonic acid and similar matter in purity over 90 %. The present invention is significant in utilizing gingko resource and protecting ecologic environment.

The invention discloses lucid ganoderma-gingko wine, relating to the technical field of biological engineering. According to the wine provided by the invention, the alcoholic strength is 8-16%, the content of general flavone is 1-5 mg / L, the content of gingko lactone is 1-6 mg / L, the content of general terpenes compound is 30-800 mg / L, the content of protein is 60-200 mg / L, the content of ganoderic acid is 10-17 mg / L, the content of soluble sugar is 0-2.0%, the content of gingko phenolic acid is 0-5 ppm and the content of hydrocyanic acid is 0-0.1 microgram / liter. According to the preparationmethod provided by the invention, the gingko is hydrolyzed by utilizing a double-enzyme method to obtain a gingko hydrolyzing solution and the gingko hydrolyzing solution contains active compounds including gingko flavone, terpenes compounds, amino acid, peptides and starch and the like, wherein the content of the amino acid and the peptides is not less than 1% and the content of the starch is not less than 2%; the hydrolyzing solution is converted through lucid ganoderma strains and a rice mold culture is added to ferment to obtain fermentation liquor; the fermentation liquor is filtered andblended to obtain the lucid ganoderma-gingko wine. The wine provided by the invention contains the active components of the gingko and the lucid ganoderma and has the functions of preventing hypertension, resisting oxidization and the like.

The invention discloses an insecticide prepared from active ingredients of ginkgo biloba testa. The insecticide mainly comprises two active ingredients including ginkgolic acid and tartaric acid whichare extracted from the ginkgo biloba testa. By limiting a dosage relationship between the two active ingredients of ginkgolic acid and tartaric acid and regulating and adjusting a pH value of a finally prepared insecticide solution, the prepared insecticide has a significantly enhanced killing effect on plant, fruit and vegetable pests, such as lipaphis erysimi, plutella xylostella, panonychus citri and the like. In addition, the invention also provides a method for preparing the insecticide from the active ingredients of the ginkgo biloba testa, high-purity selective extraction of high-efficiency insecticidal components including ginkgolic acid and tartaric acid from the ginkgo biloba testa can be achieved, and meanwhile protection of bioactivity of ginkgolic acid and tartaric acid in the production process is ensured, so that the highly effective and environmentally friendly organic insecticide is prepared. The method is simple in operation, raw materials are environmentally friendly and easy to obtain, and the formula for preparing a product is simple. In addition, the insecticide has high efficiency, low toxicity, no residues and no pollution and is more conducive to ecological balance protection.

The invention discloses a hydrophobic deep eutectic solvent for removing ginkgolic acid in a ginkgo leaf crude extract through liquid-liquid micro-extraction as well as a preparation method thereof and a removal method. The hydrophobic deep eutectic solvent is prepared from menthol, hexyl alcohol and lauric acid according to a molar ratio of 1: 0.9: 0.1 to 1: 0.4: 0.6. The hydrophobic deep eutectic solvent is simple and rapid to prepare. The hydrogen bonds are stable, the fluidity is good, and the solvent is a green solvent and has the characteristics of easy biodegradation, no environmental pollution, and high biocompatibility. The hydrophobic deep eutectic solvent can be used in a method for removing ginkgolic acid in a ginkgo leaf crude extract through liquid-liquid micro-extraction, the content of ginkgolic acid in the ginkgo leaf crude extract can be reduced to 5ppm or less; meanwhile, the content of functional components such as gingko flavonoid and terpene lactones in the gingkoleaf crude extract is maintained, and the whole liquid-liquid micro-extraction removal method is simple, convenient, quick and safe to operate, high in removal efficiency, low in cost and free of harm to the environment and human bodies.

The present invention discloses a process for extracting ginkgo biloba flavone, ginkgo biloba lactone and ginkgo biloba phenolic acid from ginkgo biloba leaves. The process comprises the following steps: (1) carrying out a pretreatment on ginkgo biloba leaves; (2) carrying out extraction by an ultrasonic device; (3) carrying out reduced pressure concentration; (4) loading the resulting solution on a macroporous resin to carry out a treatment; (5) collecting the resulting eluate; (6) carrying out reduced pressure concentration; (7) detecting the resulting extract; and (8) carrying out drying and storing on the extract. With the process of the present invention, the ginkgo biloba flavone content and the ginkgo biloba lactone content in the extract prepared by the process of the present invention are high, the loss of active ingredients are avoided, the production process is simplified, the production cycle is shortened, and the ginkgo biloba phenolic acid content after treatment is substantially reduced.

The invention relates to a method for extracting gingko powder from gingkoes and preparing gingko healthy wine, comprising the following steps of: 1, selecting high-quality gingko fruit (gingkoes); 2, husking the fruit and removing red skins and fruit cores in a sterile room; 3, taking fruit flesh and pulverizing the fruit flesh into fine paste shape fruit flesh paste; and 4, pouring the fruit flesh paste into 50% of white spirit (the proportion of the paste to the white spirit is 1 to 5), uniformly stirring the mixture, separating the white spirit and a white sediment by using a filtration method after the mixture is deposited for 8 to 10 days, and obtaining gingko fruit wine and the white sediment and drying the white sediment to obtain gingko phenol. The proportion of distiller yeast to glutinous rice to gingko leaves to water in a formulation is 1 to 8 to 2 to 19. The operation method comprises the following steps: washing gingko leaves, drying the gingko leaves, boiling the leaves with water for 15 minutes, squeezing out leaf juice, boiling the leaf juice and the glutinous rice after being washed into porridge, cooling the porridge to 25 DEG C, adding the distiller yeast into the porridge, pouring the mixture into a wine crock, brewing the gingko leaf wine after 10 to 15 days, and obtaining the gingko leaf wine by filtration; blending the gingko fruit wine and the ginkgo leaf wine according to the need to obtain the gingko healthy wine. The gingko healthy wine has abundant nutrition, has a natural health-care function and can resist and cure diseases, delay consenescence and benefit longevity after being drunk for a long time.

The invention discloses a novel ginkgo dipyridamole composition and a preparation method of a preparation thereof. The ginkgo dipyridamole composition mainly comprises the following ingredients by weight: 25-35% of ginkgo total flavonoid glycol glycosides, 10-15% of dipyridamole and 10-18% of ginkgo total lactones, wherein the ginkgo total lactones are composed of bilobalide, ginkgolides A, ginkgolides B, ginkgolides C and ginkgolides M according to the weight ratio of 22-30: 15-18: 5-8: 8-22: 4-8. The ginkgo dipyridamole composition is high in active ingredient content and low in volume of contained impurities of ginkgo biloba phenolic substances (such as ginkgolic acid), proteins, tannins, pyrogen materials and the like. The pharmaceutic preparation, especially injection prepared by theginkgo dipyridamole composition is suitable for preventing and treating coronary diseases, thromboembolic diseases and the like, has obvious curative effects, and is safe in clinic use.

The present invention is extractant, extraction process and analysis method of high-activity ginkgo leaf extractive. The key point of the present invention is one developed extractant for water extraction process of ginkgetin and EGb and the process has no step of eliminating ginkolic acid. These also includes one efficient liquid-phase chromatographic method for the quantitative analysis of ginkolic acid with secondary chemical balance. The product contains total flavone glycoside 24-32%, total terpenoid lactone 6-13%, protocyanidine 8-14% and ginkolic acid less than 5 ppm, and has yield of 2.8-3.6%. The extractant has low cost, small consumption, no residue, high stripping rate of flavones, terpenoid lactones and protocyanidin compound and has stripping of ginkolic acid.

The invention relates to application of gingkolic acid in preparation of an external preparation for treating osmidrosis. The gingkolic acid comprises ginkgoic acid, ginkgol, ginkgo diphenol and the like, and can be extracted from leaf, fruit, external exotesta, rhizome and other parts of ginkgo through a chemical separation method. The osmidrosis includes the osmidrosis of axillary part, foot part, perineum and other parts. The drug, cosmetic or disinfectant external preparation containing gingkolic acid for treating osmidrosis contains 0.001-80wt% of gingkolic acid and a pharmaceutically common external preparation matrix or acceptable carrier; and the preparation is any one pharmaceutically external dosage form. Pharmacological experiment researches show that the external preparation has the effects of inhibiting the growth and propagation of bacteria on skin surface and permeating into skin to destroy large sudoriferous gland, and inhibiting the generation of free fatty acid, ammonia nitrogen compound and other smelling substances in sweat. Clinical trial shows that the external preparation has significant treatment effect on the osmidrosis, has effective rate up to 100%, is safe to use and has no side effect.

The invention discloses a method for extracting ginkgo flavonoids from ginkgo leaves. The method comprises the steps: (1) pre-treating the ginkgo leaves; (2) performing extraction with an ultrasonic device; (3) performing concentration under reduced pressure; (4) performing treatment through macroporous resin; (5) collecting an eluate; (6) performing concentration under reduced pressure; (7) detecting an extract; and (8) performing drying and storage on the extract obtained through the steps. The obtained extract has high content of ginkgo flavonoids and ginkgolides, loss of active ingredientscan be avoided, the production process is simplified, the production cycle can be shortened, and meanwhile, the content of ginkgolic acids obtained after treatment is reduced greatly.

The invention discloses a method for extracting ginkgolide from ginkgo leaves. The method comprises the following steps: (1) carrying out earlier-stage treatment on ginkgo leaves; (2) carrying out ultrasonic device extraction; (3) carrying out vacuum concentration; (4) carrying out macroporous resin treatment; (5) collecting an eluate; (6) carrying out vacuum concentration; (7) detecting extract;and (8) drying and storing the extract extracted in the previous step. The contents of ginkgetin and ginkgolide in the extract obtained by the method are high, the loss of active ingredients is avoided, the production process is simplified, the production period is shortened, and meanwhile, the content of treated ginkgolic acid is greatly reduced.

The invention discloses a method for reducing the content of phenolic acid in a Folium Ginkgo extract, and belongs to the medicine processing field. The method comprises the following steps: drying and crushing Folium Ginkgo, sieving by a 80 mesh sieve, carrying out acoustomagnetic radiation extraction of the obtained processed Folium Ginkgo in water (added with sodium carbonate or citric acid for adjusting the pH value), mixing obtained extract liquids, concentrating, filtering, allowing the obtained filtrate to go through a macro-porous resin, concentrating the obtained alcohol phase, and detecting the content of phenolic acid in the extract through using an HPLC-UV process. The method has the advantages of energy saving, small production space, and reduction of the content of gingkolic acid in the Folium Ginkgo extract.

The invention relates to a use of ginkgolic acid nanometer liposome in preparation of external application preparations for treatment of gynecological diseases and venereal diseases. According to the present invention, the ginkgolic acid can be obtained from extracts of the exopleura part of gingko through a chemical separation method; the ginkgolic acid nanometer liposome is prepared by using a film ultrasound-high pressure homogenization method; the gynecological diseases are infectious and inflammatory genitalia diseases; the drug or the disinfectant external application preparation containing the ginkgolic acid nanometer liposome and provided for treatment of gynecological diseases and venereal diseases contains 0.001-80% of the ginkgolic acid nanometer liposome and a pharmaceutically and commonly used external application preparation substrate or an acceptable carrier; the preparation is the any one external application formulation in the pharmacy; and results of pharmacology studies and clinical trials show that the ginkgolic acid nanometer liposome provides prominent treatment effects for a variety of gynecological diseases and venereal diseases, wherein the effective rate is 100%, and characteristics of safe use and no side effect are provided.

The invention relates to application of ginkgol C17:1 in treatment of liver cancer, belonging to the field of biological drugs. The ginkgol C17:1 is prepared through a method comprising the following steps: preparing ginkgolic acid from gingko (leaves, fruits and episperm); performing heat treatment to decarboxylate the ginkgolic acid; and separating, and purifying to obtain the ginkgol C17:1, wherein structural identification shows that the ginkgol C17:1 has cis / trans isomeric structures. The prepared ginkgol C17:1 can effectively inhibit proliferation of human liver cancer cells SMMC-7721 and inhibit migration of the SMMC-7721 cells in vitro, and drug resistance can not be easily produced; and the prepared ginkgol C17:1 can inhibit growth of nude mouse loaded HepG solid tumors in vivo. The new application of the ginkgol C17:1 provided by the invention is of great importance in preparation of antitumor drugs and especially in preparation of anti-liver cancer drugs.

The invention particularly discloses a process method for preparing a high-purity ginkgolic acid extract from ginkgo outer seed coats. The method comprises the following steps: soaking the ginkgo outer seed coats, and grinding the ginkgo outer seed coats into slurry with the granularity of 6.5-13 microns; and respectively extracting the slurry with ethanol and petroleum ether, recovering the solvents, then, merging the extracts, adding deionized water into the merged extract, carrying out full stirring, adding polyamide, carrying out adsorbing twice, eluting the adsorbed polyamide for three times with ethanol, recovering the ethanol to obtain a thick-paste-shaped alcohol extract, drying the alcohol extract in vacuum, and carrying out crushing to obtain light brown yellow powder, wherein the content of the ginkgolic acid in the final product is greater than 90wt%. The high-purity ginkgolic acid extract can be used as a pharmaceutical preparation, a cosmetic product, a biopesticide and a biopesticide preparation.

The invention relates to application of gingkolic acid in preparation of an external preparation for treating osmidrosis. The gingkolic acid comprises ginkgoic acid, ginkgol, ginkgo diphenol and the like, and can be extracted from leaf, fruit, external exotesta, rhizome and other parts of ginkgo through a chemical separation method. The osmidrosis includes the osmidrosis of axillary part, foot part, perineum and other parts. The drug, cosmetic or disinfectant external preparation containing gingkolic acid for treating osmidrosis contains 0.001-80wt% of gingkolic acid and a pharmaceutically common external preparation matrix or acceptable carrier; and the preparation is any one pharmaceutically external dosage form. Pharmacological experiment researches show that the external preparation has the effects of inhibiting the growth and propagation of bacteria on skin surface and permeating into skin to destroy large sudoriferous gland, and inhibiting the generation of free fatty acid, ammonia nitrogen compound and other smelling substances in sweat. Clinical trial shows that the external preparation has significant treatment effect on the osmidrosis, has effective rate up to 100%, is safe to use and has no side effect.

The invention relates to a production method of a water-soluble ginkgo biloba extract. The production method comprises the following steps of (1) carrying out percolation extraction by using an ethanol aqueous solution; (2) concentrating and cooling the extracting solution; (3) performing centrifuging, finely filtering and removing impurities; (4) carrying out ester precipitation on a mixed solvent; (5) performing water precipitation; (6) performing deacidifying; and (7) performing drying to obtain the water-soluble high-lactone ginkgo biloba extract. According to the obtained water-soluble high-lactone ginkgo biloba extract, the flavone content is greater than or equal to 24.0%, the lactone content is greater than or equal to 10.0%, the normal-temperature water solubility is greater than10%, and the ginkgolic acid content is less than or equal to 1ppm. The produced ginkgo biloba extract is good in water solubility, and the content of lactone in the product is greatly increased. The production cost is low, the operation is simple, and the method is suitable for large-scale production.

The invention discloses a novel ginkgo dipyridamole composition and a preparation method of a preparation thereof. The ginkgo dipyridamole composition mainly comprises the following ingredients by weight: 25-35% of ginkgo total flavonoid glycol glycosides, 10-15% of dipyridamole and 10-18% of ginkgo total lactones, wherein the ginkgo total lactones are composed of bilobalide, ginkgolides A, ginkgolides B, ginkgolides C and ginkgolides M according to the weight ratio of 22-30: 15-18: 5-8: 8-22: 4-8. The ginkgo dipyridamole composition is high in active ingredient content and low in volume of contained impurities of ginkgo biloba phenolic substances (such as ginkgolic acid), proteins, tannins, pyrogen materials and the like. The pharmaceutic preparation, especially injection prepared by the ginkgo dipyridamole composition is suitable for preventing and treating coronary diseases, thromboembolic diseases and the like, has obvious curative effects, and is safe in clinic use.

The invention discloses a method for extracting ginkgolic acid from ginkgo leaves. The method comprises the steps: (1) carrying out preliminary treatment on the ginkgo leaves; (2) carrying out extracting by an ultrasonic device; (3) carrying out depressurized concentration; (4) carrying out macroporous resin treatment; (5) collecting eluate; (6) carrying out depressurized concentration; (7) detecting an extract; and (8) subjecting the extract extracted through the above-mentioned steps to drying and storing. The content of ginkgo flavones and ginkgolides in the extract obtained through the method is high, loss of active ingredients is avoided, the production process is simplified, the production cycle is shortened, and meanwhile, the content of treated ginkgolic acid is lowered greatly.

The invention discloses lucid ganoderma-gingko wine, relating to the technical field of biological engineering. According to the wine provided by the invention, the alcoholic strength is 8-16%, the content of general flavone is 1-5 mg / L, the content of gingko lactone is 1-6 mg / L, the content of general terpenes compound is 30-800 mg / L, the content of protein is 60-200 mg / L, the content of ganoderic acid is 10-17 mg / L, the content of soluble sugar is 0-2.0%, the content of gingko phenolic acid is 0-5 ppm and the content of hydrocyanic acid is 0-0.1 microgram / liter. According to the preparationmethod provided by the invention, the gingko is hydrolyzed by utilizing a double-enzyme method to obtain a gingko hydrolyzing solution and the gingko hydrolyzing solution contains active compounds including gingko flavone, terpenes compounds, amino acid, peptides and starch and the like, wherein the content of the amino acid and the peptides is not less than 1% and the content of the starch is not less than 2%; the hydrolyzing solution is converted through lucid ganoderma strains and a rice mold culture is added to ferment to obtain fermentation liquor; the fermentation liquor is filtered andblended to obtain the lucid ganoderma-gingko wine. The wine provided by the invention contains the active components of the gingko and the lucid ganoderma and has the functions of preventing hypertension, resisting oxidization and the like.

The invention discloses a specific modified product of ginkgolic acid (C13: 0) and dehydroabietylamine with anti-cancer activity, namely a preparation method of an amide compound of ginkgolic acid andapplication of the amide compound of ginkgolic acid in the aspect of anti-cancer activity. The preparation method of the amide compound is easy to operate. According to the invention, active compounds in two economic forest species are combined, so the amide compound is good in innovation. The IC50 value of the amide compound to Hela cells is 1.83 [mu]M, the IC50 value of the amide compound to HepG2 cells is 3.31 [mu]M, the IC50 value of the amide compound to MCF-7 cells is 9.98 [mu]M, and the IC50 value of the amide compound to A549 cells is 5.80 [mu]M. The amide compound has good anti-Helacell, anti-HepG2 cell, anti-MCF-7 cell and anti-A549 cell activity, has low toxicity, changes the traditional concept that ginkgo biloba is toxic and causes the phenomena of anaphylactic reactions, even death and the like if eaten without cooking or eaten excessively, and provides a new idea for the development of anticancer drugs. The compound is novel in structure, contains a long-chain electrondonating group (-C13H27), has low toxicity and unique anti-cancer activity, is an anti-cancer drug with an excellent prospect, creates conditions for researching the relationship between the anti-cancer activity and the structure of the compound, and lays a foundation for research and development of new drugs.

The invention relates to an ultrasonic extraction, purification and extraction method of ginkgol, and belongs to the technical field of natural plant active ingredient extraction. The method comprisesthe following steps: carrying out strong ultrasonic fragmentation on fresh gingko kernels, mixing with water, filtering, carrying out solvent extraction, and finally heating to a ginkgol gasificationtemperature to obtain an oily substance, namely the ginkgol. The method for independently extracting the ginkgol is provided for the first time, the method is simple in process, low in cost and goodin effect, the required component ginkgol can be effectively enriched and separated, and the quality and content of the ginkgol extract are improved.

The invention discloses ginkgo biloba soybean paste and a preparation method thereof. The ginkgo biloba soybean paste is prepared from the following raw materials in parts by weight: 50-100 parts of soybeans, 40-80 parts of ginkgo biloba, 10-20 parts of flour, 0.6-0.9 part of aspergillus oryzae, 0-0.3 part of aspergillus niger, 8-16 parts of table salt, 15-30 parts of chilies, 8-16 parts of white granulated sugar and 0.3-0.6 part of xanthan gum. According to the soybean paste disclosed by the invention, the content of the ginkgo biloba is high, ginkgo biloba fruits contain active ingredients such as flavone, lactone, white tartaric acid, ginkgol, bilobol, bacteriostatic proteins and polysaccharide, and the soybean paste has the effects of inhibiting fungi, improving brain functions, resisting anoxia, resisting fatigue, delaying senescence and the like, and has very high nutritional value. The ginkgo biloba soybean paste is ruddy in color and luster, rich in sauce fragrance, obvious in delicate flavor, palatable in salty and sweet taste, moderate in thickness and free of impurity particles, and development of the ginkgo biloba soybean paste can enrich the variety of thick broad-bean paste and open up a new way for deep processing of the ginkgo biloba. The preparation method is simple in process and low in cost.

The invention discloses a method for liquid state fermentation of Saccharomyces coronis and the product and application thereof. The method comprises: activating and separating Saccharomyces coronis, and preparing spore suspension; making ginkgo fruit into ginkgo nuts , adding water to grind, adding inorganic salts to prepare a liquid fermentation medium; inoculating the spore suspension of Cystis coronis in the liquid fermentation medium, and vibrating and culturing to obtain a liquid fermentation product of Cystis coronis. In the present invention, ginkgo is used as a liquid fermentation medium for the coronoid fungus, and after fermentation, most of the nutritional components and functional components of the ginkgo are retained, and the contents of ginkgolic acid and MPN are reduced. In addition, after fermentation, it contains a large number of spores of Cystis coronis, lovastatin, polysaccharides, α-amylase, protease and many other functional health ingredients, and the flavor is obviously improved. Its ginkgo fermented products can be used to prepare ginkgo products such as enzymes and beverages with the functions of regulating blood lipid, regulating immunity, anti-oxidation and anti-tumor.

The invention discloses a hydrophobic deep eutectic solvent used for liquid-liquid microextraction to remove ginkgolic acids in ginkgo biloba crude extract, a preparation method and a removal method thereof, wherein the hydrophobic deep eutectic solvent consists of menthol, hexanol and Lauric acid is prepared at a molar ratio of 1:0.9:0.1 to 1:0.4:0.6. The preparation of the hydrophobic deep eutectic solvent of the present invention is simple and fast; the hydrogen bond is stable, the fluidity is good, it is a green solvent, and has the characteristics of easy biodegradation, no pollution to the environment, and high biocompatibility; the hydrophobic deep eutectic solvent prepared by the present invention The method of liquid-liquid microextraction to remove ginkgolic acids in the crude extract of ginkgo leaves can reduce the content of ginkgolic acids in the crude extract of ginkgo leaves to less than 5ppm, while retaining the content of ginkgo flavonoids and terpenes in the crude extract of ginkgo leaves The content of functional components such as esters, the whole liquid-liquid micro-extraction removal method is simple, fast, safe, high removal efficiency, low cost, and will not cause harm to the environment and human body.

The invention discloses a ginkgolic acid-rhamnolipid compound bactericide, a preparation method and application thereof, and belongs to the technical field of pesticides. The invention is prepared from the following raw materials in parts by weight: 15-25 parts of ginkgolic acid extract, 10-15 parts of rhamnolipid, 3-5 parts of synergist, 1-3 parts of wetting agent, and dispersant 1-3 parts, 0.5-1 parts of preservatives, 0.5-0.8 parts of antifreeze and 10-15 parts of fillers. The invention adopts the combination of membrane technology and macroporous adsorption resin to separate and purify the ginkgolic acid, and realizes the high-efficiency extraction of the ginkgolic acid. The present invention adds houttuynia cordata and bamboo vinegar liquid as synergists, and the two cooperate with each other to promote the fungicide of the present invention to exert the bactericidal effect, and meanwhile, the bamboo vinegar also has the effect of promoting the germination, rooting and growth of crop seeds and improving crop yield. The invention selects discarded ginkgo leaf waste residue and outer testa as raw materials to prepare the fungicide, which reduces the use of chemical pesticides and reduces environmental pollution.