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Process method for preparing high-purity ginkgolic acid extract from ginkgo outer seed coats

The technology of Ginkgo biloba seed coat and ginkgo phenolic acid is applied in the fields of medicine and biological pesticide processing, and can solve the problems of complicated process, troublesome operation and high impurity content, and achieve the effects of simple extraction process, production cost saving and good separation effect.

Inactive Publication Date: 2021-12-24
江苏蓝星生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In China, organic solutions such as ethanol, methanol, and petroleum ether are commonly used as extraction or refined solvents to extract ginkgolic acids. There are also processes such as ion-exchange resin adsorption and elution for extraction of ginkgolic acids. The extraction of organic solutions generally has low extraction rates. The impurity content is high, the content and purity of ginkgolic acid are relatively low, and the content and purity of ginkgolic acid produced by ion exchange method are relatively high, but the process is relatively complicated, the production cost is high, and resin regeneration requires a large amount of acid-base treatment, not only The operation is troublesome, and a large amount of acid and lye will flow out as waste liquid, causing environmental pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Step (1) Weigh 1 kg of the picked and dried ginkgo exotesta, add 10 kg of deionized water, soak for 24 hours at a temperature of 41°C, and filter out the soaking water after soaking;

[0023] Step (2) Add 1 kg of deionized water to the ginkgo testa soaked in step (1), grind it into a coarse pulp with a refiner, and then finely grind the coarse pulp with a colloid mill to obtain a finely ground slurry , the particle size of the obtained slurry is 6.5-13 μm;

[0024] Step (3) Add 20 kg of ethanol with a content concentration of 98% V / V to the finely ground slurry obtained in step (2), extract for 12 hours at a temperature of 43°C, and filter the ethanol extract; add oil to the filter residue Ether 20kg, at a temperature of 30°C, leaching for 18 hours, filtering the petroleum ether extract, and then recovering ethanol and petroleum ether from the ethanol extract and petroleum ether extract, respectively, to obtain a creamy ethanol extract With the cream petroleum ether ex...

Embodiment 2

[0030] Step (1) Weigh 1 kg of the picked and dried ginkgo exotesta, add 10 kg of deionized water, and soak for 20 hours at a temperature of 42°C. After soaking, filter out the soaking water;

[0031] Step (2) Add 1 kg of deionized water to the ginkgo testa soaked in step (1), grind it into a coarse pulp with a refiner, and then finely grind the coarse pulp with a colloid mill to obtain a finely ground slurry , the particle size of the obtained slurry is 6.5-13 μm;

[0032] Step (3) Add 20 kg of ethanol with a content concentration of 98% V / V to the finely ground slurry obtained in step (2), extract for 10 hours at a temperature of 44°C, and filter the ethanol extract; add oil to the filter residue Ether 20kg, at a temperature of 36°C, leaching for 18 hours, filtering the petroleum ether extract, and then recovering ethanol and petroleum ether from the ethanol extract and petroleum ether extract, respectively, to obtain a creamy ethanol extract With the cream petroleum ether ext...

Embodiment 3

[0038] Step (1) Weigh 1 kg of the picked and dried ginkgo exotesta, add 10 kg of deionized water, and soak for 18 hours at a temperature of 43°C. After soaking, filter out the soaking water;

[0039] Step (2) Add 1 kg of deionized water to the ginkgo testa soaked in step (1), grind it into a coarse pulp with a refiner, and then finely grind the coarse pulp with a colloid mill to obtain a finely ground slurry , the particle size of the obtained slurry is 6.5-13 μm;

[0040] Step (3) Add 20 kg of ethanol with a content concentration of 98% V / V to the finely ground slurry obtained in step (2), extract for 8 hours at a temperature of 45°C, and filter the ethanol extract; add oil to the filter residue Ether 20kg, at a temperature of 33°C, leaching for 18 hours, filtering the petroleum ether extract, and then recovering ethanol and petroleum ether from the ethanol extract and petroleum ether extract, respectively, to obtain a creamy ethanol extract With the cream petroleum ether ex...

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Abstract

The invention particularly discloses a process method for preparing a high-purity ginkgolic acid extract from ginkgo outer seed coats. The method comprises the following steps: soaking the ginkgo outer seed coats, and grinding the ginkgo outer seed coats into slurry with the granularity of 6.5-13 microns; and respectively extracting the slurry with ethanol and petroleum ether, recovering the solvents, then, merging the extracts, adding deionized water into the merged extract, carrying out full stirring, adding polyamide, carrying out adsorbing twice, eluting the adsorbed polyamide for three times with ethanol, recovering the ethanol to obtain a thick-paste-shaped alcohol extract, drying the alcohol extract in vacuum, and carrying out crushing to obtain light brown yellow powder, wherein the content of the ginkgolic acid in the final product is greater than 90wt%. The high-purity ginkgolic acid extract can be used as a pharmaceutical preparation, a cosmetic product, a biopesticide and a biopesticide preparation.

Description

technical field [0001] The invention belongs to the field of medicine and bio-pesticide processing, and relates to the preparation of ginkgolic acid extract contained in medicine and bio-pesticide, in particular to a process for preparing high-purity ginkgolic acid extract by using ginkgo exotesta. Background technique [0002] Ginkgophenolic acid mainly comes from the leaves, fruits, testa and rhizomes of Ginkgo biloba, among which the content in the testa of Ginkgo is the highest, accounting for about 3-5% of the testa of Ginkgo biloba. Ginkgolic acid has a strong killing or inhibiting effect on fungi, and also has a good killing effect on some diseases and insect pests. Therefore, in the field of medicine, it has been developed into a medicament and is widely used in dermatology, gynecology and other related diseases. Prevention and treatment, there are still applications in cosmetics, which have good control effects on agricultural pathogens and pests, so they are made i...

Claims

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Application Information

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IPC IPC(8): A61K36/16A61P31/10A61K8/9771A61Q17/00A01N65/06A01P7/04A61K131/00
CPCA61K36/16A61P31/10A61K8/9771A61Q17/005A01N65/00A01N65/06A61K2236/331A61K2236/333A61K2236/35A61K2236/55A61K2800/805
Inventor 杨宪勇张爱丽花丽
Owner 江苏蓝星生物工程有限公司
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