Application of ginkgolic acid nanometer liposome in treatment of gynecological diseases and venereal diseases

A technology of nanoliposome and ginkgolic acid, applied in the field of biomedicine, can solve the problems such as no reports on the application of ginkgolic acid nanoliposome, and achieve the effects of no skin irritation, significant therapeutic effect, and inhibition of proliferation

Inactive Publication Date: 2014-04-30
王青
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] There is no report on the application of ginkgolic acid nanolip

Method used

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  • Application of ginkgolic acid nanometer liposome in treatment of gynecological diseases and venereal diseases
  • Application of ginkgolic acid nanometer liposome in treatment of gynecological diseases and venereal diseases
  • Application of ginkgolic acid nanometer liposome in treatment of gynecological diseases and venereal diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The extraction process of ginkgolic acid from the ginkgo fruit outer testa is as follows: the ginkgo fruit outer testa is peeled off, dried in the sun, and pulverized with a pulverizer. Accurately weigh a certain amount of crushed ginkgo testa, add 80% ethanol with a material-to-liquid ratio of 1:6 (W / V), and stir and extract twice at a water bath temperature of 60°C, each extraction time is 4 hours . After the leaching is finished, filter with a suction filter to collect the filtrate. Adsorption and purification by D101 macroporous resin, desorption by 10BV of 100% ethanol, purification by silica gel column chromatography, eluting with petroleum ether: ethyl acetate (90:10, V / V), and concentrating the eluate with a vacuum rotary evaporator , the conditions are temperature 55°C, pressure 0.008-0.009MPa, and concentration under reduced pressure. Vacuum-dry at 60°C to obtain ginkgolic acid extract. The properties are: light brown extract, slightly smelly, soluble in alc...

Embodiment 2

[0029] The extraction process and content determination method of ginkgolic acid in the outer testa of ginkgo fruit were the same as in Example 1, and the ginkgoolic acid extract was obtained, and the content of ginkgophenolic acid was 924.51 mg / g.

[0030] Ginkgolic acid nanoliposome suspension was prepared according to the preparation process of ginkgolic acid nanoliposome suspension in Example 1.

[0031]Preparation of ointment: Take 5ml of the above-mentioned ginkgolic acid nanoliposome suspension, dissolve it in 100ml of 95% ethanol solution, add 50ml of triethanolamine to it, and heat to 70°C. Add 300g of paraffin wax, 300g of stearic acid, and 100ml of propylene glycol, respectively, and stir to melt, add distilled water to a sufficient amount of 1000g, and stir evenly until it becomes a paste. The plastid content is 0.5%. Packed in 50 small tubes, each 20 g.

[0032] The ointment preparation process is a known and common method.

Embodiment 3

[0034] The extraction process and content determination method of ginkgophenolic acid in the outer testa of ginkgo fruit are the same as in Example 1 to obtain a ginkgophenolic acid extract with a content of gingkgolic acid of 945.07 mg / g.

[0035] Ginkgolic acid nanoliposome suspension was prepared according to the preparation process of ginkgolic acid nanoliposome suspension in Example 1.

[0036] The preparation of tincture: get above-mentioned ginkgolic acid nano liposome suspension 100ml, be dissolved in 600ml 95% ethanol solution, then add triethanolamine 50ml respectively wherein, propylene glycol 200ml stir, make up 95% ethanol solution to a sufficient amount of 1000 ml, That is, 1000 ml of light yellow ginkgolic acid nanoliposome tincture was obtained, and the content of ginkgolic acid nanoliposome suspension was 10%. Packed in 50 small tubes, each 20 ml.

[0037] The tincture preparation process is a well-known public method.

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Abstract

The invention relates to a use of ginkgolic acid nanometer liposome in preparation of external application preparations for treatment of gynecological diseases and venereal diseases. According to the present invention, the ginkgolic acid can be obtained from extracts of the exopleura part of gingko through a chemical separation method; the ginkgolic acid nanometer liposome is prepared by using a film ultrasound-high pressure homogenization method; the gynecological diseases are infectious and inflammatory genitalia diseases; the drug or the disinfectant external application preparation containing the ginkgolic acid nanometer liposome and provided for treatment of gynecological diseases and venereal diseases contains 0.001-80% of the ginkgolic acid nanometer liposome and a pharmaceutically and commonly used external application preparation substrate or an acceptable carrier; the preparation is the any one external application formulation in the pharmacy; and results of pharmacology studies and clinical trials show that the ginkgolic acid nanometer liposome provides prominent treatment effects for a variety of gynecological diseases and venereal diseases, wherein the effective rate is 100%, and characteristics of safe use and no side effect are provided.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to the use of ginkgolic acid nano liposomes in preparing external preparations for treating gynecological diseases and venereal diseases. Background technique [0002] Gynecological diseases and venereal diseases are one of the most common clinical diseases, and there is an increasing trend in various places year by year. Because such diseases last for a long time and are easy to recur, they bring pain to patients and seriously affect their physical and mental health. These diseases are mostly related to genital and surrounding resident pathogen infection, such as gonorrhea, herpes virus, human papillomavirus (HPV), Treponema pallidum, pubic lice and other infections, causing a variety of sexually transmitted diseases such as gonorrhea, genital herpes, genital warts, Syphilis, pubic lice, etc.; gynecological diseases such as trichomonas vaginitis, fungal vaginitis, b...

Claims

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Application Information

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IPC IPC(8): A61K9/127A61K36/16A61K31/60A61P15/00A61P15/02A61P31/04A61P31/10A61P31/20A61P33/02A61P31/22A61P33/14A61K31/05
Inventor 雷红王青
Owner 王青
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