A hydrophobic deep eutectic solvent used for liquid-liquid microextraction to remove ginkgolic acid in ginkgo biloba crude extract and its preparation method and removal method

Abstract

The invention discloses a hydrophobic deep eutectic solvent used for liquid-liquid microextraction to remove ginkgolic acids in ginkgo biloba crude extract, a preparation method and a removal method thereof, wherein the hydrophobic deep eutectic solvent consists of menthol, hexanol and Lauric acid is prepared at a molar ratio of 1:0.9:0.1 to 1:0.4:0.6. The preparation of the hydrophobic deep eutectic solvent of the present invention is simple and fast; the hydrogen bond is stable, the fluidity is good, it is a green solvent, and has the characteristics of easy biodegradation, no pollution to the environment, and high biocompatibility; the hydrophobic deep eutectic solvent prepared by the present invention The method of liquid-liquid microextraction to remove ginkgolic acids in the crude extract of ginkgo leaves can reduce the content of ginkgolic acids in the crude extract of ginkgo leaves to less than 5ppm, while retaining the content of ginkgo flavonoids and terpenes in the crude extract of ginkgo leaves The content of functional components such as esters, the whole liquid-liquid micro-extraction removal method is simple, fast, safe, high removal efficiency, low cost, and will not cause harm to the environment and human body.

Description

technical field [0001] The invention relates to the processing and utilization of agricultural and forestry biological resources, in particular to a hydrophobic deep eutectic solvent used for liquid-liquid micro-extraction to remove ginkgolic acid in ginkgo biloba crude extract, and a preparation method and removal method thereof. Background technique [0002] Ginkgo biloba L. is a deciduous tree of the genus Ginkgo in the family Ginkgoaceae, and there is only one family and one genus in the Ginkgoles. The appearance of Ginkgo biloba can be traced back to hundreds of millions of years ago. It is the oldest relict plant among the gymnosperms left over from the Quaternary glacial movement, and is called a "living fossil". Ginkgo biloba is a multi-purpose tree species unique to my country for medicine, food, material and ornamental, and it is a rare plant under the second-class protection of the country. my country is the origin of ginkgo, and it is also the largest ginkgo pla...

AI Technical Summary

Problems solved by technology

[0005] Purpose of the invention: for the defects existing in the prior art, the present invention provides a new hydrophobic deep eutectic solvent for liquid-liquid microextraction to remove ginkgolic acid in the crude extract of ginkgo biloba, and the hydrophobic deep eutectic solvent can Efficient and simple method for realizing the removal of ginkgophenolic acid, while retaining the content of ginkgo flavonoids and terpene lactones to a large extent; it solves the problem of large amount of organic solvent usage and complicated operation in the existing method of removing ginkgolic acid , high cost, low efficiency and a series of problems

Images