Rhynchophylline solid lipid nanoparticles and its preparation method and freeze-dried powder preparation
A technology of solid lipid nanometer and rhynchophylline, which is applied in freeze-dried transportation, powder transportation, pharmaceutical formulations, etc., can solve the problems of poor solubility of rhynchophylline and achieve good stability and uniform distribution
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Embodiment 1
[0039] Weigh 50mg glyceryl monostearate, 50mg Tween 80 and 100mg Solutol HS 15 (polyethylene glycol-15 hydroxystearate), heat and melt at 80°C to form an oil phase, and accurately weigh 2.00mg rhynchophylline Dissolve in the oil phase; take another 10ml of purified water and heat it to 80°C as the water phase; after the oil phase is completely melted, quickly add the water phase into the oil phase, stir at 2000r / min for 5min, and the nanoemulsion can be formed. Then place it in an ice-water bath to cool to obtain rhynchophylline solid lipid nanoparticle suspension.
[0040] The encapsulation efficiency and drug loading capacity of rhynchophylline solid lipid nanoparticles were determined by ultrafiltration: take 0.5ml of the prepared rhynchophylline solid lipid nanoparticle suspension, add ultrapure water to dilute to 3ml, and mix well Place in ultrafiltration tube, 3000r / min, centrifuge 5min, take supernatant, under chromatographic conditions (chromatographic column uses octa...
Embodiment 2
[0051] Adding a lyoprotectant before making rhynchophylline SLN into freeze-dried powder is to prevent rhynchophylline SLN from being destroyed during the freeze-drying process, and on the other hand, to obtain a dense and full appearance, no color difference, and layer, lyophilized powder with smaller particle size.
[0052] In this example, the best lyoprotectants were screened from mannitol, sucrose, glucose and trehalose, and their dosages and ratios are shown in Table 1.
[0053] The experimental operation for preparing rhynchophylline SLN freeze-dried powder in this embodiment is: use an analytical balance to weigh 15 prescription amounts of lyoprotectants (in % (w / v) of rhynchophylline SLN suspension) according to Table 1 ), add in 15 cillin bottles successively, then draw 2ml prepared rhynchophylline SLN suspensions with pipette gun respectively and add in the above-mentioned cillin bottles successively. Use a pipette gun to draw 2ml of the prepared rhynchophylline SL...
Embodiment 3
[0066] Weigh 500mg glyceryl monostearate, 50mg Tween 80 and 50mg Solutol HS 15 (polyethylene glycol-15 hydroxystearate), heat and melt at 65°C to form an oil phase, and accurately weigh 6.0mg rhynchophylline Dissolve in the oil phase; take another 3.0ml of purified water and heat it to 65°C as the water phase; after the oil phase is completely melted, quickly add the water phase into the oil phase and stir at 50r / min for 15min to form a nanoemulsion , and then cooled in an ice-water bath to obtain rhynchophylline solid lipid nanoparticle suspension.
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