Unlock instant, AI-driven research and patent intelligence for your innovation.

A method for identifying whether the body fluid to be tested is non-blood, menstrual blood or peripheral blood and the miRNA combination used therefor

A peripheral blood and menstrual blood technology, applied in the field of miRNA combination, can solve the problems of incomplete consistency and poor repeatability of results

Active Publication Date: 2022-05-10
INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there have been a large number of studies using miRNA to identify the source of body fluid tissue. However, the conclusions drawn by different research methods and experimental platforms are not completely consistent, and the reproducibility of the results is poor.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for identifying whether the body fluid to be tested is non-blood, menstrual blood or peripheral blood and the miRNA combination used therefor
  • A method for identifying whether the body fluid to be tested is non-blood, menstrual blood or peripheral blood and the miRNA combination used therefor
  • A method for identifying whether the body fluid to be tested is non-blood, menstrual blood or peripheral blood and the miRNA combination used therefor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0100] 1. Sample collection

[0101] The 200 samples came from 200 volunteers (aged 23-35) in Beijing and Hangzhou. 200 volunteers were unrelated individuals, and all of them gave informed consent.

[0102] The 40 samples numbered PB1-PB40 are all peripheral blood samples. Peripheral blood samples were obtained from venous blood drawn from the arms of some volunteers and stored at -80°C.

[0103] The 40 samples numbered MB1-MB40 are all menstrual blood samples. The 40 menstrual blood samples were collected by some volunteers with tampons 4 days before the menstrual cycle, dried at room temperature for 1 day, and stored at -80°C.

[0104] The 40 samples numbered SA1-SA40 are all saliva samples. The 40 saliva samples were obtained by collecting saliva from some volunteers in sterile plastic tubes (the volunteers fasted for 1 h before collection), and stored at -80°C.

[0105] The 40 samples with sample numbers SE1-SE40 are all semen samples. 40 semen samples were obtained ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for identifying whether a body fluid to be tested is non-blood, menstrual blood or peripheral blood and the miRNA combination used therein. The method is to detect the relative expression of miR-451, miR-203, miR-205, and miR-214 in the body fluid to be tested, and then substitute it into the Fisher discriminant function to judge whether the body fluid to be tested is non-blood, menstrual blood or peripheral blood. Sex can reach 100%. The invention provides accurate scientific basis for clarifying the nature of the case, determining the criminal suspect, conviction and sentencing, etc., and has important application value.

Description

technical field [0001] The invention belongs to the field of forensic technology, and in particular relates to a method for identifying whether a body fluid to be tested is non-blood, menstrual blood or peripheral blood and the miRNA combination used therein. Background technique [0002] By identifying the tissue source of bodily fluid stains at the crime scene, the source of the sample and its real criminal activities can be inferred, thereby providing important information for case characterization and scene reconstruction, and important scientific evidence for forensic science. Common bodily fluids at the scene of the case include peripheral blood, menstrual blood, saliva, semen, and vaginal secretions. Blood is one of the most common and important samples at a crime scene. The biggest difference between blood and non-blood samples is that blood contains a large number of blood cells. Although peripheral blood and menstrual blood contain a large number of red blood cells...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6876C12N15/113
CPCC12Q1/6876C12Q2600/158C12Q2600/178
Inventor 孙启凡季安全赵一霞韩娜刘耀叶健何红霞
Owner INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY