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adsl gene and its genetic marker method for Xupu aninosinic acid content
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A technique of anisinic acid and genetic marker is applied in the field of determination of polymorphism in ADSL gene, and can solve the problem that genetic markers have not yet been reported.
Active Publication Date: 2022-06-21
HUNAN AGRICULTURAL UNIV
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Problems solved by technology
[0006] The research on DNA genetic markers, genetic diversity and related functional genes of Xupu goose is still in its infancy, and the genetic markers related to IMP in Xupu goose have not been reported yet.
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Embodiment 1
[0036] Embodiment 1 Xupu goose pectoralis muscle inosinic acid related gene SNP screening
[0037] 60 healthy Xupu geese of the same age were selected for feeding experiment. After 28 days of slaughter, the inosinic acid content in the breast muscle was detected by high performance liquid chromatography, and the genomic DNA was extracted from the samples with high and low inosinic acid content, respectively.
[0038] According to the goose ADSL gene sequence in GenBank, primers were designed with Primer5.0 to cover the entire sequence of exon 9 to exon 10 of the gene. The primer sequences are:
[0039] Forward primer: 5'-CACCCATAAAGGGAACAG-3'
[0040] Reverse primer: 5'-AGCACAGACAGCCAGATT-3'
[0041] Primers were synthesized by Hunan Qingke Biotechnology Co., Ltd. The Xupu goose genomic DNA was amplified with the above primers.
[0042] PCR: 20 μl reaction system: 12 μl of 2×Master mix, 0.8 μl of upstream and downstream primers (10 μmol / L)), 1 μl of DNA template, and suppl...
Embodiment 2
[0047] Embodiment 2 Verification of SNP relevant to Xupu goose pectoralis muscle inosinic acid content in production practice
[0048] Twenty healthy Xupu geese at 80 days of age and under the same feeding and management conditions were selected for the experiment. After 28 days of feeding, all the tested Xupu geese were slaughtered, and the content of inosinic acid in the breast muscle was detected.
[0049] Extracted 20 Xupu goose breast muscle tissue-like genomic DNAs were amplified using the primers in Example 1, and their SNPs were detected according to the method in Example 1.
[0050] Table 1 PCR reaction system
[0051]
[0052] PCR cycling parameters are as follows:
[0053] Pre-denaturation at 95 °C for 5 min; denaturation at 95 °C for 30 s, annealing at 57.6 °C for 30 s, extension at 72 °C for 45 s, a total of 35 cycles; extension at 72 °C for 10 min, and finally storage at 4 °C.
[0054] Genotype frequency analysis of ADSL gene SNP loci
[0055] The genotype...
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Abstract
The invention discloses an adenosine succinate lyase (ADSL) gene and its use as a genetic marker for the content of inosinic acid in the muscle of Xupu goose. Goose ADSL gene polymorphism, and then the correlation analysis between ADSL gene polymorphism and pectoral inosinic acidity, found that in Xupu goose ADSL gene, there is a G / T mutation at 146bp, GG and TT The content of inosinic acid in the pectoral inosinic acid of Xupu goose genotype was significantly higher than that of GT genotype. The invention provides molecular markers for the marker-assisted selection of the inosinic acidity traits of Xupu goose pectoralis.
Description
technical field [0001] The invention belongs to the field of poultry molecular biotechnology and breeding, and is mainly used for the determination of genetic markers related to the content of inosinic acid in muscles of Xupu geese, specifically, to determine the polymorphism in the ADSL gene, that is, to determine the polymorphism in Xupu geese The presence of a single nucleotide polymorphism (Singlenucleotide polymorphism, abbreviated as SNP) in the adenosuccinate lyase gene associated with inosinic acidity. [0002] technical background [0003] As a local breed resource, Xupu goose has delicious meat and is rich in essential nutrients for human body, which is very beneficial to human health. Goose meat is rich in nutrients, rich in various amino acids, proteins, vitamins, sugars, and trace elements necessary for the human body, and has low fat content and high unsaturated fatty acid content, which is easy to be digested and absorbed by the human body. In 2002, the Food a...
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