Evanescent wave two-colour fluorescence all-fibre biosensor

Abstract

The invention relates to an evanescent wave two-colour fluorescence all-fibre biosensor. The evanescent wave two-colour fluorescence all-fibre biosensor is characterized by comprising a laser, an optical switch, a single-mode and multi-mode optical fibre coupler, a sample tank, an optical fibre probe, a photoelectric detector and a control system; two input ports of the optical switch are separately connected to the laser; the single-mode optical fibre of the single-mode and multi-mode optical fibre coupler is connected to the output port of the optical switch, and used for transferring excitation light and stimulating and acquiring fluorescence; an anion scale remover and a to-be-detected sample labelled by two fluorescent dyes are put in the sample tank; two biological molecules are modified on the optical fibre probe; the optical fibre probe is put in the sample tank; the optical fibre probe is connected to the multi-mode optical fibre of the single-mode and multi-mode optical fibre coupler; the photoelectric detector is connected to the output end of the single-mode and multi-mode optical fibre coupler; a double band pass filter is arranged between the photoelectric detector and the single-mode and multi-mode optical fibre coupler; and the control system is electrically connected to the photoelectric detector. The evanescent wave two-colour fluorescence all-fibre biosensor in the invention can be widely used in the technical field of environment monitoring.

Description

technical field [0001] The invention relates to an all-fiber biosensor, in particular to an evanescent wave two-color fluorescence all-fiber biosensor, which belongs to the technical field of environmental monitoring. Background technique [0002] The overall quality of the water environment is not high, and frequent pollution incidents are the core of the water environment crisis. In recent years, frequently exposed water pollution incidents have become a bottleneck restricting social development. Water pollution incidents have been banned repeatedly, posing potential hazards to the ecological environment and human health, and causing serious social and economic impacts. At present, the monitoring technology of toxic pollutants in the water environment requires the use of traditional laboratory instrument analysis methods such as gas chromatography and high performance liquid chromatography. These large-scale laboratory instrument analysis methods have the advantages of hi...

AI Technical Summary

Problems solved by technology

However, the existing multi-index sensing methods based on evanescent waves mostly rely on complex and expensive detection instruments, and most of them adopt the mode of "separate excitation, separate detection", which has great difficulties in real-time detection

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