A kind of chitosan nano material and preparation method thereof for lung cancer treatment
A technology of chitosan nano and chitosan, which is applied in the field of biomedicine, can solve problems such as poor water solubility, and achieve the effect of overcoming drug resistance
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Embodiment 1
[0037] 20 mg of N-4-azido-phthalimido-chitosan CS-N 3 (purchased from Shanghai Boao Biotechnology Co., Ltd., chitosan with a molecular weight of 60 KDa and a deacetylation degree of 90% as raw materials) was added to 2 mL of dimethyl sulfoxide, stirred until it was completely dissolved, and then placed in a flask 4 mg Er, 16 mg Q-amine and 4 mg of Cy7 were added to The above system was placed in a nitrogen atmosphere, and 200 μL of an aqueous solution of copper sulfate pentahydrate (20 mg / mL) and 200 μL of an aqueous solution of sodium vitamin C (15 mg / mL) were sequentially added dropwise to the flask with a 1 mL syringe. Wrap the flask with foil and stir at room temperature for three days. The reaction solution was taken out and put into a dialysis bag (8000-14000 Da) for dialysis for three days. The dialysate was taken out and freeze-dried to obtain CE7Q. Infrared spectrum analysis of CE7Q, such as figure 1 Shown, Cs-N 3 at 2100 cm -1 There is a strong absorption peak ...
Embodiment 2
[0039] 21 mg of N-4-azido-phthalimido-chitosan CS-N 3 (purchased from Shanghai Boao Biotechnology Co., Ltd., chitosan with a molecular weight of 60 KDa and a deacetylation degree of 90% as raw materials) was added to 2 mL of dimethyl sulfoxide, stirred until it was completely dissolved, and then placed in a flask Added 3 mg Er, 27 mg Q-amine and 3 mg of Cy7. The above system was placed in a nitrogen atmosphere, and 200 μL of an aqueous solution of copper sulfate pentahydrate (20 mg / mL) and 200 μL of an aqueous solution of sodium vitamin C (15 mg / mL) were added dropwise to the flask with a 1 mL syringe. Wrap the flask with foil and stir at room temperature for three days. The reaction solution was taken out and put into a dialysis bag (8000-14000 Da) for dialysis for three days. The dialysate was taken out and freeze-dried to obtain CE7Q. The grafting rates of Er, Cy7 and Q-amine in CE7Q were calculated to be 10%, 10% and 80%, respectively.
Embodiment 3
[0041] 20 mg of N-4-azido-phthalimido-chitosan CS-N 3 (purchased from Shanghai Boao Biotechnology Co., Ltd., chitosan with a molecular weight of 60 KDa and a deacetylation degree of 90% as raw materials) was added to 2 mL of dimethyl sulfoxide, stirred until it was completely dissolved, and then placed in a flask 7 mg Er, 6 mg Q-amine and 10 mg of Cy7 were added to The above system was placed in a nitrogen atmosphere, and 200 μL of an aqueous solution of copper sulfate pentahydrate (20 mg / mL) and 200 μL of an aqueous solution of sodium vitamin C (15 mg / mL) were sequentially added dropwise to the flask with a 1 mL syringe. Wrap the flask with foil and stir at room temperature for three days. The reaction solution was taken out and put into a dialysis bag (8000-14000 Da) for dialysis for three days. The dialysate was taken out and freeze-dried to obtain CE7Q. The grafting rates of Er, Cy7 and Q-amine in CE7Q were calculated to be 40%, 40% and 20%, respectively.
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