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Method for simultaneously determining 20 organic amines, biogenic amines and cations in fruit juice beverage

A technology of organic amines and biogenic amines, applied in the field of analytical chemistry, can solve problems such as large interference, poor stability and sensitivity, complicated pretreatment and operation steps, etc., to improve detection sensitivity and accuracy, improve detection accuracy, improve Measuring the effect of productivity

Active Publication Date: 2019-09-20
日照海关综合技术服务中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the ICP method and electrophoresis method have problems such as large interference, poor stability and sensitivity, and there are deficiencies such as pretreatment and complicated operation steps.

Method used

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  • Method for simultaneously determining 20 organic amines, biogenic amines and cations in fruit juice beverage
  • Method for simultaneously determining 20 organic amines, biogenic amines and cations in fruit juice beverage
  • Method for simultaneously determining 20 organic amines, biogenic amines and cations in fruit juice beverage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1. A method for simultaneous determination of 20 organic amines, biogenic amines and cations in fruit juice beverages, including the following steps:

[0052] Step 1. Prepare standard mixed working fluid:

[0053] Inorganic cations are all standard solutions Li purchased from the National Testing Center for Nonferrous Metals and Analytical Materials + , Na + , NH 4 + , K + , Mg 2+ , Ca 2+ , Sr 2+ , Ba 2+ , The ion concentration is 1000 mg / L, the concentration of the standard solution of methylamine and dimethylamine is 1000 mg / L, the concentration of the standard solution of trimethylamine is 2000 mg / L, diethanolamine, butylamine, histamine, cadaverine, putrefaction Amine, agmatine, heptyldiamine, spermidine, and spermine are standard substances with a purity of ≥97%; in turn, the standard substances or solutions of organic amines and biological amines are configured into standard solutions with a mass concentration of 1000 mg / L. When in use, pipette a certain amount...

Embodiment 2

[0076] Example 2: According to the experimental analysis method in Example 1, the standard mixed working solution was measured under the flow rate conditions of 0.20 mL / min, 0.25 mL / min, and 0.30 mL / min.

[0077] Since the initial concentration of methanesulfonic acid in the gradient condition is small, the pressure at this time is the highest in the entire gradient elution step. After that, as the concentration of methanesulfonic acid in the mobile phase increases, the pressure gradually decreases. When the flow rate is 0.20 mL / min, the initial pressure is 9.9 MPa. At this time, the pressure is smaller, the peak width increases, the peak sensitivity decreases, and the overall resolution is not high. At a flow rate of 0.25 mL / min, the separation effect of the components is generally good, and the initial pressure at a flow rate of 0.25 mL / min is 12.0 MPa, which is moderate. The initial pressure is 15.1 MPa at a flow rate of 0.30 mL / min. At this time, the pressure is relatively hi...

Embodiment 3

[0078] Example 3. According to the experimental analysis method in Example 1, the initial concentrations of the methanesulfonic acid eluent were 1.0 mmol / L, 1.5 mmol / L, 1.75 mmol / L, 2.0 mmol / L, 2.5 mmol / L, respectively. Under the conditions, determine the standard mixed working solution. The ion chromatograms of standard mixed working solutions at different initial concentrations are as follows figure 2 Shown

[0079] From figure 2 It can be seen that the smaller the initial concentration, the longer the peak time of the components, especially the first 8 components have the most obvious changes; at this time, the overall resolution increases, but the response value of the chromatographic peak decreases, and the peak sensitivity decreases. The greater the initial concentration, the shorter the peak time of various components, and the overall resolution decreases, but the response value of the chromatographic peak becomes larger and the sensitivity of the chromatographic peak in...

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Abstract

The invention discloses a method for simultaneously determining 20 organic amines, biogenic amines and cations in a fruit juice beverage, belonging to the field of analytical chemistry. In the method, a conductivity inhibition-ion chromatography method is adopted, and under the condition of multi-stage gradient elution, the experimental determination conditions are optimized by researching a plurality of experimental influence factors, so that the method for simultaneously determining 20 organic amines, biogenic amines and cations (methylamine, dimethylamine, trimethylamine, diethanolamine, butylamine, histamine, cadaverine, putrescine, agmatine, heptanediamine, spermidine, spermine, Li+, Na+, NH4+, K+, Mg2+, Ca2+, Sr2+ and Ba2+) in the fruit juice beverage is established. The method is rapid, simple and convenient, high in high in sensitivity and good in stability, and achieves the aims of rapid analysis, green analysis and multi-component simultaneous analysis.

Description

Technical field [0001] The invention belongs to the field of analytical chemistry, and specifically relates to a method for simultaneously determining 20 kinds of organic amines, biological amines and cations in fruit juice beverages. Background technique [0002] Biogenic amines are a class of nitrogen-containing aliphatic or heterocyclic organic compounds with low molecular weight and are widely found in aquatic products, vegetables, beer, wine and other foods. Biogenic amines are not only precursors for the production of hormones, nucleic acids, and proteins, but also for the production of carcinogenic nitroso compounds. Appropriate intake of biogenic amines can effectively regulate physiological activities in the body, such as appropriate intake of spermidine. It can delay memory loss and prevent the onset of Alzheimer's disease; however, excessive intake of certain biogenic amines can cause adverse physiological reactions such as headaches and abdominal cramps in the body, w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8809G01N2030/884
Inventor 宋卫得苏征鞠玲燕朱万燕林令海张传杰
Owner 日照海关综合技术服务中心
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