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miR-451a antisense strand inhibitor and application thereof

A technology of mir-451a and 3.mir-451a, applied in the field of biomedicine, can solve the problem that the action and mechanism of miR-451a have not yet been reported, and achieve the effect of protecting liver function, reducing alcoholic fatty liver disease and reducing fat accumulation

Active Publication Date: 2019-10-08
THE FIRST AFFILIATED HOSPITAL OF GUANGXI MEDICAL UNIV
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Problems solved by technology

The above research results indicate that miRNA plays an important role in the occurrence and development of ALD, but the role and mechanism of miR-451a in alcoholic liver disease have not been reported yet

Method used

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  • miR-451a antisense strand inhibitor and application thereof
  • miR-451a antisense strand inhibitor and application thereof
  • miR-451a antisense strand inhibitor and application thereof

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Embodiment 1

[0019] 1. Preparation of mouse alcoholic liver disease (ALD) model and detection of liver function indicators

[0020] (1) Refer to NIAAA law. Twelve female wild-type C57BL / 6 mice, about 8 weeks old, were randomly divided into two groups. The mice in the experimental group (EtOH-fed) were fed with Lieber-DeCarli liquid feed for 5 days and fed with Lieber-DeCarli liquid feed containing 5% alcohol for 10 days. Nine hours before the anesthesia was sacrificed, intragastric administration (31.5% ethanol, 20 μL / g mouse) was added. Mice in the control group (pair-fed) were fed with non-alcoholic Lieber-DeCarli liquid feed, and 9 hours before anesthesia and sacrificed, a gavage (45% dextrin, 20 μL / g mouse) was added once. The whole process includes liquid feed adaptation period (5 days), modeling period (10 days), gavage (1 time) and specimen collection.

[0021] (2) Take the venous blood of the mice, separate and obtain the serum, and detect the content of liver function biochemic...

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Abstract

The invention discloses a miR-451a antisense strand inhibitor and an application thereof. According to the invention, a mouse model with alcoholic liver disease is established, deep sequencing of small RNA on the liver tissues of a model group and a normal control group can be performed, the differentially expressed miR-451a can be analyzed and screened, and qRT-PCR is used for verification. the miR-451a antisense strand inhibitor is chemically synthesized, in-vivo experiments in mice is carried out, the results show that the miR-451a antisense strand inhibitor effectively reduces ALT and glutamic-pyruvic transaminase AST in serum of mice with alcoholic fatty liver disease, and the accumulation of fat in the liver is significantly reduced, which indicates that the miR-451a antisense chaininhibitor can protect liver function and alleviate alcoholic fatty liver disease. The miR-451a antisense strand inhibitor is expected to be developed as a new small molecule RNA drug for the treatmentof alcoholic fatty liver disease.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to a miR-451a antisense chain inhibitor and application thereof. Background technique [0002] Alcoholic fatty liver is a common liver disease in western countries and belongs to a subtype of alcoholic liver disease (alcoholic liver disease, ALD). In recent years, the incidence of alcoholic liver disease in my country has shown an obvious upward trend, and it is often combined with hepatitis virus infection, causing serious harm to people's health. At present, its pathogenesis is not clear, and there is still a lack of effective treatment. [0003] The liver is the main site of ethanol metabolism. About 90% of the alcohol ingested by the body is metabolized by the liver. Alcohol is mainly metabolized by three enzyme systems in the liver: alcohol dehydrogenase system (ADH), microsomal ethanol oxidation system (MEOS) and peroxidase in peroxisomes. Alcohol inhibits A...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7125A61P1/16A23L33/13
CPCA23V2002/00A61K31/7125A23L33/13A61P1/16A23V2200/30
Inventor 何松青钟伏弟江克清吴展
Owner THE FIRST AFFILIATED HOSPITAL OF GUANGXI MEDICAL UNIV
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