Recombinant human follicle-stimulating hormone and its preparation method

Abstract

The invention provides a recombinant human follicle-stimulating hormone and a preparation method thereof. Specifically, steps are included: (1) the first expression cassette for expressing FSH α subunit and the second expression cassette for expressing FSH β subunit are operatively inserted into the eukaryotic expression vector to obtain the protein capable of expressing FSH α subunit and the transfection vector of FSH beta subunit protein; (2) transforming the transfection vector into eukaryotic cells, thereby obtaining the transformed eukaryotic cells integrated with the first expression cassette and the second expression cassette in the chromosome; (3) Under conditions suitable for expression, the transformed eukaryotic cells are cultivated to express the FSHα subunit and the FSHβ subunit, thereby obtaining a fermentation product containing recombinant follicle-stimulating hormone; (4) to the described The fermentation product is separated and purified to obtain the recombinant follicle-stimulating hormone. The method of the invention can replace the use and preparation method of the existing human FSH, and obtain rhFSH with significantly improved activity and purity.

Description

technical field

[0001] The invention belongs to the technical field of proteins, and in particular relates to a polypeptide with follicle hormone activity and a preparation method for the polypeptide. Background technique

[0002] Low FSH concentration due to insufficient FSH synthesis and secretion is the main cause of female and male infertility. In women, this condition is characterized by anovulation or abnormal ovulation; in men, infertility may result from insufficient production of viable spermatozoa. In the diagnosis and treatment of infertility, FSH is a bioactive molecule that plays an important role. FSH plays an important role in human assisted reproductive technology. It is the main drug used to treat male and female infertility in the market. One of the drugs.

[0003] FSH is a heterodimeric glycoprotein with a molecular weight of about 31KD, composed of non-covalently bonded α subunits and β subunits, of which the relative molecular weight of the α subunit i...

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