Hematoxylin dye solution for quick frozen sections used in linear dyeing instrument and dyeing method
A frozen section and hematoxylin technology, which is applied in the field of biomedicine, can solve the problems of blurred frozen sections, light staining of nuclei, easy fading of sections, etc., and achieves the effects of obvious contrast, elimination of eosin co-staining, and increase of nuclear staining strength.
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Embodiment 1
[0049] Materials and Methods
[0050] 1.1 The source tissue of the specimen comes from the fresh tissue for rapid pathological examination during the daily inspection of the Department of Pathology, Renhe Hospital, Baoshan District, Shanghai.
[0051] 1.2 Equipment and reagents Leica ST4020 linear stainer (Leica, Germany), glass rod, hematoxylin, aluminum sulfate, sodium iodate, alcohol-soluble eosin Y, 75% ethanol, 95% ethanol, absolute ethanol, xylene, hydrochloric acid, distilled water.
[0052] 1.3 Method: Improving the preparation of dye solution, that is, the preparation of hematoxylin stain solution for quick frozen sections of linear staining instrument: hematoxylin 2.8g, absolute ethanol 250mL, aluminum sulfate 24.6g, distilled water 750mL, sodium iodate 0.2g, glacial acetic acid 15mL. Dissolve hematoxylin in absolute ethanol, dissolve aluminum sulfate in distilled water, mix the two liquids after fully dissolving, and add sodium iodate in time, add glacial acetic ac...
Embodiment 2
[0074] The hematoxylin staining solution for quick frozen sections of the linear stainer is made of the following components: 2.4g hematoxylin, 200mL absolute ethanol, 20g aluminum sulfate, 800mL distilled water, 0.2g sodium iodate, and 15mL glacial acetic acid.
[0075] The staining method using the above-mentioned hematoxylin staining solution for the quick frozen section of the linear stainer is the same as in Example 1, and the results are as follows Figure 5 as shown, Figure 5 It is a schematic diagram of the quick-frozen section stained with the dye solution prepared in Example 2 observed under a microscope.
Embodiment 3
[0077] The hematoxylin staining solution for quick frozen sections of the linear stainer is made of the following components: hematoxylin 3.5g, absolute ethanol 300mL, aluminum sulfate 38g, distilled water 700mL, sodium iodate 0.3g, glacial acetic acid 15mL.
[0078] The staining method using the above-mentioned hematoxylin staining solution for the quick frozen section of the linear stainer is the same as in Example 1, and the results are as follows Figure 6 as shown, Figure 6 It is a schematic diagram of observing under a microscope the quick frozen sections stained with the dye solution prepared in Example 3.
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