Hematoxylin dye solution for quick frozen sections used in linear dyeing instrument and dyeing method

A frozen section and hematoxylin technology, which is applied in the field of biomedicine, can solve the problems of blurred frozen sections, light staining of nuclei, easy fading of sections, etc., and achieves the effects of obvious contrast, elimination of eosin co-staining, and increase of nuclear staining strength.

Active Publication Date: 2019-10-22
SHANGHAI BAOSHAN DISTRICT RENHE HOSPITAL
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  • Application Information

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Problems solved by technology

However, due to the limitations of the design of the linear staining instrument, after directly using the conventionally prepared staining solution and staining method, the nuclei are lightly stained, and the co-staining of the nucleus and cytoplasm is obvious. Frozen sections often have blurred phenomena, making diagnosis difficult, and the stained sections are easy to fade. , short storage time

Method used

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  • Hematoxylin dye solution for quick frozen sections used in linear dyeing instrument and dyeing method
  • Hematoxylin dye solution for quick frozen sections used in linear dyeing instrument and dyeing method
  • Hematoxylin dye solution for quick frozen sections used in linear dyeing instrument and dyeing method

Examples

Experimental program
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Effect test

Embodiment 1

[0049] Materials and Methods

[0050] 1.1 The source tissue of the specimen comes from the fresh tissue for rapid pathological examination during the daily inspection of the Department of Pathology, Renhe Hospital, Baoshan District, Shanghai.

[0051] 1.2 Equipment and reagents Leica ST4020 linear stainer (Leica, Germany), glass rod, hematoxylin, aluminum sulfate, sodium iodate, alcohol-soluble eosin Y, 75% ethanol, 95% ethanol, absolute ethanol, xylene, hydrochloric acid, distilled water.

[0052] 1.3 Method: Improving the preparation of dye solution, that is, the preparation of hematoxylin stain solution for quick frozen sections of linear staining instrument: hematoxylin 2.8g, absolute ethanol 250mL, aluminum sulfate 24.6g, distilled water 750mL, sodium iodate 0.2g, glacial acetic acid 15mL. Dissolve hematoxylin in absolute ethanol, dissolve aluminum sulfate in distilled water, mix the two liquids after fully dissolving, and add sodium iodate in time, add glacial acetic ac...

Embodiment 2

[0074] The hematoxylin staining solution for quick frozen sections of the linear stainer is made of the following components: 2.4g hematoxylin, 200mL absolute ethanol, 20g aluminum sulfate, 800mL distilled water, 0.2g sodium iodate, and 15mL glacial acetic acid.

[0075] The staining method using the above-mentioned hematoxylin staining solution for the quick frozen section of the linear stainer is the same as in Example 1, and the results are as follows Figure 5 as shown, Figure 5 It is a schematic diagram of the quick-frozen section stained with the dye solution prepared in Example 2 observed under a microscope.

Embodiment 3

[0077] The hematoxylin staining solution for quick frozen sections of the linear stainer is made of the following components: hematoxylin 3.5g, absolute ethanol 300mL, aluminum sulfate 38g, distilled water 700mL, sodium iodate 0.3g, glacial acetic acid 15mL.

[0078] The staining method using the above-mentioned hematoxylin staining solution for the quick frozen section of the linear stainer is the same as in Example 1, and the results are as follows Figure 6 as shown, Figure 6 It is a schematic diagram of observing under a microscope the quick frozen sections stained with the dye solution prepared in Example 3.

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Abstract

The invention discloses a dye solution for quick frozen sections used in a linear dyeing instrument and a dyeing method. The dye solution is made of the following components: 2.4-3.5g of hematoxylin,200-300ml of anhydrous ethanol, 20-38g of aluminum sulfate, 700-800ml of distilled water, 0.2-0.3g of sodium iodate and 13-20ml of glacial acetic acid. In the dyeing method, hydrochloric acid ethanolis removed, and a xylene cylinder is added. By using the dye solution for quick frozen sectioning of a linear dyeing instrument and the dyeing method, after a linear dyeing instrument dyes a quick frozen section, the nuclear coloring power can be increased, and the phenomena of hematoxylin co-dyeing and eosin co-dyeing can be eliminated. Therefore, it is ensured that the structures of the cell nucleus and cytoplasm are clear, the contrast is obvious, the dyeing time is reduced, the color is not easy to fade, the storage time of the frozen section is greatly prolonged, the phenomenon of fuzziness under the microscope is eliminated, the dyeing time in the process of quick frozen sectioning is standardized, and the dyeing quality is improved.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a hematoxylin dye solution and a dyeing method for quick frozen sections of a linear stainer. Background technique [0002] Fast frozen section provides powerful help for rapid intraoperative biopsy pathological diagnosis, and can distinguish the nature of the lesion (benign, borderline, malignant), or tumor invasion at the resection margin, whether the lymph node has tumor metastasis, etc., and provide surgeons with an appropriate surgical plan Provide evidence. Due to the constraints of many factors during the rapid freezing and diagnosis during the operation, delayed diagnosis often occurs. Especially when multiple cases or multiple parts are sent for inspection in a centralized manner for quick freezing, the limited technicians are at a loss. The introduction of linear staining instrument gradually replaced manual staining, solved the problem of lack of pers...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30
CPCG01N1/30
Inventor 陈荣范宜娟杨滢瑜潘晓燕汤芃芃
Owner SHANGHAI BAOSHAN DISTRICT RENHE HOSPITAL
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