Method for detecting residues of amantadine, rimantadine and memantine in animal derived food

Abstract

The disclosure provides a method for detecting residues of amantadine, rimantadine and memantine in an animal derived food. The method comprises the steps of: (1) taking a sample to add into recombinant solution of trichloroacetic acid and acetonitrile to carry out vortex oscillation, carrying out centrifugation after ultrasonic treatment, transferring supernatant A for additional storage, addingthe recombinant solution of the trichloroacetic acid and the acetonitrile into remaining residues to carry out vortex oscillation, carrying out centrifugation after ultrasonic treatment, taking out supernatant B to merge with the supernatant A, and then blow-drying to obtain sample extraction solution residues; (2) carrying out complex solution vertex oscillation on the extraction solution residues, adding C18, PAS and neutral alumina, carrying out centrifugation after ultrasonic treatment, and filtering supernatant through a filter membrane so as to obtain a to-be-detected sample; and (3) detecting content of the amantadine, the rimantadine and the memantine in the to-be-detected sample by utilizing an ultra-high performance liquid chromatography tandom mass spectrometer. According to themethod, the difficult problems of more interference and low recovery rate when the amantadine, the rimantadine and the memantine are simultaneously detected are effectively solved; cost is saved; andworking efficiency is improved.

Description

technical field [0001] The present disclosure relates to the field of food safety, in particular to a method for detecting residues of amantadine, rimantadine and memantine in animal-derived foods. Background technique [0002] With the rapid progress of society, people's living standards have been greatly improved, and animal food has gradually become a rigid need in daily life. People's market demand for animal food has driven the expansion of food animal breeding. Blind expansion of low-scale breeding has led to the complication of food-borne animal diseases and confusion in the supervision of the veterinary drug market. Memantine, etc. are used for food animals, but these drugs have not been approved for veterinary use. [0003] In 2005, the U.S. Food and Drug Administration (FDA) ordered to prohibit the abuse of human antiviral drugs in livestock and poultry. At the same time, the No. 560 Announcement of the Ministry of Agriculture of my country also explicitly prohib...

AI Technical Summary

Problems solved by technology

[0004] However, the existing detection methods for antiviral drug residues in livestock and poultry meat are relatively old, and the detection items and matrices are relatively single. Each antiviral drug detection method is different, and the instruments and reagents used have different degrees of In addition, there are large differences in the detection range and precision of each method. Even some antiviral drugs still lack detection methods, which has caused problems such as high detection cost, long cycle, and great difficulty.

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