Hydrophobic material surface modification method and application of material after modification

A surface modification, hydrophobic material technology, applied in analytical materials, instruments, measuring devices, etc., can solve the problems of complex experimental conditions, multi-step process flow, unfavorable technical methods are widely used and popularized, and long-term effects cannot be guaranteed. Adhesion and cell culture effect, improvement of micron-scale cell manipulation and detection and analysis application, effect of good modification effect

Active Publication Date: 2019-11-12
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the conventional methods of physical modification of cell adhesion molecules can only temporarily improve the cell adhesion on the surface of hydrophobic materials, the stability is poor, and the long-term effect cannot be guaranteed and realized; most of the chemical modification methods require harsh experimental conditions and complex multi-step processes Processes that are not conducive to the widespread use and popularization of technical methods by stakeholders in multidisciplinary fields

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  • Hydrophobic material surface modification method and application of material after modification
  • Hydrophobic material surface modification method and application of material after modification
  • Hydrophobic material surface modification method and application of material after modification

Examples

Experimental program
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Effect test

Embodiment 1

[0029] This embodiment provides a modification method that can enhance the adhesion of nerve cells on the surface of PDMS hydrophobic materials, which specifically includes the following steps:

[0030] In this embodiment, in addition to the modification method involved in the present invention, two control groups (unmodified group and PDL modified group) were also set up to compare the cell adhesion effect.

[0031] Three pieces of PDMS membranes were placed in three regular 35mm cell culture dishes.

[0032] In Dish 1 (unmodified group), the PDMS membrane was washed for 3 min with fresh neuron cell basal culture medium (Gibco).

[0033] In dish 2 (PDL modification group), 100 μg / mL PDL solution was added to the surface of PDMS, 2 mL / dish, incubated at 20°C for 2 hours, and then washed with fresh cell culture medium for 3 minutes.

[0034] In Dish 3 (the present invention relates to a modification method), 100 μg / mL PDL-F127 solution was added to the surface of PDMS, 2 mL / di...

Embodiment 2

[0041] This embodiment provides the nerve cell patterning application of the PDMS hydrophobic material surface modification method in Example 1, which specifically includes the following steps:

[0042] First, if image 3 As shown in A, the neuron patterning device that can be used for microfluidic pipelines is assembled, that is, the PDMS microchannel layer (31) is placed on a PDMS substrate layer (32), and the PDMS microchannel layer adopts conventional soft lithography technology In preparation, the micropipe layer comprises parallel micropipes (33), inlets (34), and outlets (35).

[0043] The device was placed in a conventional 35mm cell culture dish.

[0044] Pour 100 μg / mL PDL-F127 solution from the inlet of the microchannel layer (34) into the parallel microchannels (33), modify the inner surface of the microchannels, incubate at 20°C for 2 hours, and wash with fresh cell culture medium for 3 minutes.

[0045] Remove the PDMS micropipe layer (31), and keep the PDMS ba...

Embodiment 3

[0049] This example provides the long-term culture of nerve cells and the formation of neural networks in the microfluidic chip of the PDMS hydrophobic material surface modification method in Example 1, which specifically includes the following steps:

[0050] First, if Figure 5 As shown in A, the microfluidic chip used in this embodiment consists of a PDMS chip fluid layer (51) and a PDMS chip base layer (52) from top to bottom, wherein the chip fluid layer is prepared by conventional soft lithography technology, and the fluid layer It includes a micro-pipe network (53), a chip inlet (54), and a chip outlet (55).

[0051] The 100 μg / mL PDL-F127 solution is perfused into the micropipe network (53) from the chip inlet (54), the inner surface of the micropipe network is modified, incubated at 20° C. for 2 hours, and fresh nerve cell basal culture medium (Gibco Inc. ) for 3 minutes.

[0052] Such as Figure 5 As shown in B, the 2×10 6 Each / mL primary nerve cell (56) suspensi...

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Abstract

The invention discloses a hydrophobic material surface modification method and application of the material after modification. The method comprises the following step: performing surface contact on apolylysine coupled poloxamer solution with a hydrophobic material. According to the method disclosed by the invention, the cell adhesiveness of the surface of the hydrophobic material can be remarkably improved through one-step method surface modification, multiple steps of modification can be avoided, adhesiveness, growth culture and detection analysis capability of living cells on the surface ofthe hydrophobic material can be greatly improved, and the method can be conveniently mastered and used by researchers of multiple subjects and fields; and in addition, the modification method is simple, rapid and efficient to operate, and is applicable to various types of in-vitro living cell micro engineering operation and analysis application.

Description

technical field [0001] The invention belongs to the technical field of material surface modification, and in particular relates to a method for modifying the surface of a hydrophobic material and the application of the modified material in the aspects of living cell adhesion, cultivation, detection and analysis. Background technique [0002] Cell microengineering technology is an emerging technology that has attracted the attention of research institutions and scholars at home and abroad in this century. It can be used to carry out bionic cell manipulation and analysis of organ tissue microenvironment on various micron-scale interfaces. Its representative technologies include cell patterning technology (Cell patterning) and microfluidic cell chip technology (Microfluidic cell chip). At present, the materials used in cell micro-engineering technology mainly include: polydimethylsiloxane (PDMS), polymethyl methacrylate, polystyrene, glass, silicon wafer, etc. However, most of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J7/12C08G65/333G01N33/569C08L83/04
CPCC08G65/33324C08J7/12C08J2383/04G01N33/56966
Inventor 刘文明
Owner CENT SOUTH UNIV
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