Transgenic plant and preparation method thereof
A technology of transgenic plants and genes, applied in the field of plant genetic engineering, can solve the problems of undiscovered anti-oxidative stress, etc., and achieve the effect of accurate preparation and simple operation
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[0038] The embodiment of the present invention provides a preparation method for preparing the above-mentioned transgenic plants, the preparation method comprising:
[0039] Extract the RNA of Brachypodium distachyon;
[0040] Reverse transcription of RNA into cDNA;
[0041] Using cDNA as a template, using the first forward primer and the first reverse primer to carry out PCR amplification to obtain the amplified product, the sequence of the first forward primer is shown in SEQ ID NO: 2 in the sequence table, and the first reverse primer The sequence of the primer is shown in SEQID NO:3 in the sequence listing;
[0042] connecting the amplified product to a carrier to obtain a ligated product;
[0043] Transfer the ligated product into competent cells to obtain the transformed product;
[0044] Positive single clones were screened out from the transformation products to obtain recombinant plasmids;
[0045] Insert the DREB1-like gene on the recombinant plasmid into the bac...
Embodiment 1
[0064] An embodiment of the present invention provides a transgenic plant, the transgenic plant includes a DREB1-like gene, and the sequence of the DREB1-like gene is shown in SEQ ID NO: 1 in the sequence listing.
[0065] The transgenic plants provided by the embodiments of the present invention include the DREB1-like gene, which can endow the transgenic plants with the characteristics of anti-oxidative stress, and provide good climatic gene resources for plant breeding work, and at the same time utilize the transgene of the DREB1-like gene Studying the molecular mechanism of anti-oxidation in plants can facilitate the study of the function of their homologous genes.
Embodiment 2
[0067] The embodiment of the present invention provides a preparation method for preparing transgenic plants, the preparation method comprising:
[0068] The RNA of Brachypodium distachyon was extracted; at the time of realization, 0.1 g of fresh leaves of Brachypodium distachyon was taken, and the total RNA was extracted by the Trizol method. The source of Brachypodium distachyda is preserved by the laboratory of Jianghan University.
[0069] The RNA was reverse-transcribed into cDNA; at the time of implementation, the cDNA was obtained using a cDNA synthesis kit purchased from TaKaRa Company.
[0070] Using cDNA as a template, use the first forward primer and the first reverse primer to perform PCR (Polymerase Chain Reaction, polymerase chain reaction) amplification to obtain the amplified product pBdDREB1-like, the sequence of the first forward primer is shown in the sequence table Shown in SEQ ID NO: 2, the sequence of the first reverse primer is shown in SEQ ID NO: 3 in ...
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