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UPLC characteristic spectrum construction method and identification method of pyrrosia peduncularis, pyrrosia lingua, pyrrosia sheareri and pyrrosia huabeiensis

A kind of technology with Psilocybin and characteristic map, applied in the field of medicine, can solve the problems of insufficient description of the quality of traditional Chinese medicine, lack of specificity, deviation of conclusions, etc., and achieve the effect of stable method, good reproducibility and high accuracy

Active Publication Date: 2019-11-12
GUANGDONG YIFANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At this stage, the traditional simple methods such as seeing, tasting, sniffing, and touching have been very limited in the identification of Chinese herbal medicines due to their strong subjectivity. However, there are a large number of false positives and false positives in chromatographic methods such as UV, IR, NIR, and TLC. Negative questions can easily lead to biased conclusions
[0004] In recent years, due to the diversity and complexity of active ingredients in traditional Chinese medicine, the determination of the content of a single ingredient in the past is not enough to explain the quality of Chinese medicinal materials, and it lacks specificity

Method used

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  • UPLC characteristic spectrum construction method and identification method of pyrrosia peduncularis, pyrrosia lingua, pyrrosia sheareri and pyrrosia huabeiensis
  • UPLC characteristic spectrum construction method and identification method of pyrrosia peduncularis, pyrrosia lingua, pyrrosia sheareri and pyrrosia huabeiensis
  • UPLC characteristic spectrum construction method and identification method of pyrrosia peduncularis, pyrrosia lingua, pyrrosia sheareri and pyrrosia huabeiensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The Construction of UPLC Characteristic Maps of Cervus chinensis, Cinnamon chinensis, Cinnamon Lushan and Cinnamon chinensis

[0035](1) Accurately weigh the medicinal materials of Shiwei pediposa, Shiwei, Lushan Shiwei, and North China Shiwei to prepare the test solution of Shiwei, Shiwei, Lushan Shiwei, and North China Shiwei;

[0036] (2) Analyzing the test solution of Sylvia pedescens, Sylvia sylvestris, Lushan syphilis, and North China syphilis by ultra-high performance liquid chromatography, the UPLC of Sylvia sylvestris, S. Characteristic spectrum to determine the number of common peaks.

[0037] The 29 batches of experimental samples came from major pharmacies across the country and passed the tests of relevant authoritative institutions. See Table 1 for details.

[0038] Table 1 The batch numbers of 29 batches of Jiyuan Shiwei

[0039]

[0040] Preparation of the test samples of Shiwei, Shiwei, Lushan Shiwei, and North China Shiwei: Take 0.5g of the powder...

Embodiment 2

[0059] Identification of 5 Batches of Lushan Shiwei Medicinal Materials

[0060] The identification steps of Lushan Shiwei medicinal materials are as follows:

[0061] (1) Accurately weigh the Lushan Shiwei medicinal material to be identified, and prepare the Lushan Shiwei sample solution to be identified;

[0062] (2) Precisely absorb the sample solution of Lushan Shiwei to be identified, inject it into an ultra-high performance liquid chromatograph, measure it, and get it;

[0063] (3) Compare the measured UPLC characteristic spectrum with the constructed UPLC characteristic spectrum of Lushan Shiwei. If the number of common peaks is consistent with the Lushan Shiwei characteristic spectrum, the sample to be identified is Lushan Shiwei.

[0064] Chromatographic conditions

[0065] Use octadecylsilane bonded silica gel as filler, methanol as mobile phase A, and 0.08-0.13% phosphoric acid aqueous solution as mobile phase B to carry out gradient elution with a flow rate of 0....

Embodiment 3

[0073] Identification of 3 batches of Shiwei medicinal materials in North China

[0074] The identification steps of Shiwei medicinal materials in North China are as follows:

[0075] (1) Accurately weigh the medicinal material of Rhizoma chinensis to be identified, and prepare the sample solution of Rhizoma chinensis to be identified;

[0076] (2) Precisely absorb the sample solution of North China Shiwei to be identified, inject it into an ultra-high performance liquid chromatograph, measure it, and get it;

[0077] (3) Compare the measured UPLC characteristic spectrum with the constructed UPLC characteristic spectrum of S. chinensis. If the number of common peaks is consistent with the characteristic spectrum of S. chinensis, the sample to be identified is S. chinensis.

[0078] Chromatographic conditions

[0079] Use octadecylsilane bonded silica gel as filler, methanol as mobile phase A, and 0.08-0.13% phosphoric acid aqueous solution as mobile phase B to carry out grad...

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Abstract

The invention relates to a UPLC characteristic spectrum construction method and an identification method of pyrrosia peduncularis, pyrrosia lingua, pyrrosia sheareri and pyrrosia huabeiensis. The UPLCcharacteristic spectrum construction method of pyrrosia peduncularis, pyrrosia lingua, pyrrosia sheareri and pyrrosia huabeiensis comprises the following steps: (1) accurately weighing pyrrosia peduncularis, pyrrosia lingua, pyrrosia sheareri and pyrrosia huabeiensis, and preparing a test solution of pyrrosia peduncularis, pyrrosia lingua, pyrrosia sheareri and pyrrosia huabeiensis; and (2) analyzing the test solution of pyrrosia peduncularis, pyrrosia lingua, pyrrosia sheareri and pyrrosia huabeiensis by using an ultra-high performance liquid chromatograph to obtain UPLC characteristic spectrums of pyrrosia peduncularis, pyrrosia lingua, pyrrosia sheareri and pyrrosia huabeiensis, and determining the number of common peaks. The method has good precision, stability and reproducibility, fully shows the chemical characteristics of pyrrosia peduncularis, pyrrosia lingua, pyrrosia sheareri and pyrrosia huabeiensis, and can better identify medicinal materials of pyrrosia peduncularis, pyrrosia lingua, pyrrosia sheareri and pyrrosia huabeiensis.

Description

technical field [0001] The invention belongs to the technical field of medicines, and specifically discloses a method for constructing UPLC characteristic maps of syphilis pedunata, shiwei, Lushan shiwei and North China shiwei, and a method for identifying them. Background technique [0002] The shiwei recorded in the 2015 edition of "Chinese Pharmacopoeia" is the dried leaves of Pyrrosia sheareri (Bak.) Ching, Pyrrosia lingua (Thunb.) Farwell or Pyrrosia petiolosa (Christ) Ching . Can be harvested throughout the year, remove rhizomes and roots, and dry in the sun or in the shade. Pyrrosiapetiolosa (Christ) Ching is one of the three sources of traditional Chinese medicine Pyrrosiapetiolosa (Christ) Ching. Shiwei is sweet and bitter in taste, slightly cold, and returns to the lung and bladder meridian, and has the effects of inducing diuresis and treating stranguria, clearing the lung and relieving cough, cooling blood and stopping bleeding. Shi Wei was first recorded in "...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 魏梅李国卫杨晓东索彩仙潘礼业孙冬梅陈向东程学仁
Owner GUANGDONG YIFANG PHARMA