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A construction method and identification method of the ULC characteristic map of the peduncle, Shiwei, Lushan Shiwei and North China Shiwei

A technology of Pietophyllum and characteristic maps, applied in the field of medicine, can solve the problems of insufficient explanation of the quality of Chinese medicinal materials, lack of specificity, and deviation of conclusions, and achieve the effect of stable method, good reproducibility, and high accuracy

Active Publication Date: 2022-06-24
GUANGDONG YIFANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At this stage, the traditional simple methods such as seeing, tasting, sniffing, and touching have been very limited in the identification of Chinese herbal medicines due to their strong subjectivity. However, there are a large number of false positives and false positives in chromatographic methods such as UV, IR, NIR, and TLC. Negative questions can easily lead to biased conclusions
[0004] In recent years, due to the diversity and complexity of active ingredients in traditional Chinese medicine, the determination of the content of a single ingredient in the past is not enough to explain the quality of Chinese medicinal materials, and it lacks specificity

Method used

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  • A construction method and identification method of the ULC characteristic map of the peduncle, Shiwei, Lushan Shiwei and North China Shiwei
  • A construction method and identification method of the ULC characteristic map of the peduncle, Shiwei, Lushan Shiwei and North China Shiwei
  • A construction method and identification method of the ULC characteristic map of the peduncle, Shiwei, Lushan Shiwei and North China Shiwei

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Construction of UPLC characteristic maps of Shiwei, Shiwei, Lushan Shiwei and North China Shiwei

[0035](1) Precisely weigh the medicinal materials of Shiwei, Shiwei, Lushan Shiwei, and North China Shiwei, and prepare the test solution of Shiwei, Shiwei, Lushan Shiwei, and North China Shiwei;

[0036] (2) Analyzing the test solutions of Shiwei, Shiwei, Lushan Shiwei, and North China Shiwei by ultra-high performance liquid chromatography, and obtaining UPLC of Shiwei, Shiwei, Lushan Shiwei, and North China Shiwei Characteristic maps to determine the number of common peaks.

[0037] The 29 batches of experimental samples came from major pharmacies across the country and passed the testing of relevant authoritative institutions. See Table 1 for details.

[0038] Table 1 Batch numbers of 29 batches of Jiyuan Shiwei

[0039]

[0040] Preparation of test products of Shiwei, Shiwei, Lushan Shiwei, and North China Shiwei: Take 0.5 g of Shiwei Shiwei, Shiwei, Lushan Shiwei...

Embodiment 2

[0059] Identification of 5 Batches of Lushan Shiwei Medicinal Materials

[0060] The identification steps of Lushan Shiwei medicinal materials are:

[0061] (1) Precisely weigh the medicinal materials to be identified Lushan Shiwei, and prepare the Lushan Shiwei sample solution to be identified;

[0062] (2) Precisely draw the Lushan Shiwei sample solution to be identified, inject it into an ultra-high performance liquid chromatograph, and measure;

[0063] (3) Compare the measured UPLC characteristic map with the constructed UPLC characteristic map of Lushan Shiwei. If the number of common peaks is consistent with the Lushan Shiwei characteristic map, the sample to be identified is Lushan Shiwei.

[0064] Chromatographic conditions

[0065] Use octadecylsilane-bonded silica gel as filler, methanol as mobile phase A, and 0.08~0.13% phosphoric acid aqueous solution as mobile phase B, carry out gradient elution, the flow rate is 0.34~0.46ml per minute, and the column temperatu...

Embodiment 3

[0073] Identification of three batches of North China Shiwei medicinal materials

[0074] The identification steps of North China Shiwei medicinal materials are:

[0075] (1) Precisely weigh the medicinal materials to be identified, and prepare a sample solution of Shiwei to be identified;

[0076] (2) Precisely draw the sample solution of Shiwei from North China to be identified, inject it into an ultra-high performance liquid chromatograph, and measure;

[0077] (3) Compare and compare the measured UPLC characteristic spectrum with the constructed UPLC characteristic spectrum of Shiwei in North China. If the number of common peaks is consistent with the characteristic spectrum of Shiwei in North China, the sample to be identified is Shiwei in North China.

[0078] Chromatographic conditions

[0079] Use octadecylsilane-bonded silica gel as filler, methanol as mobile phase A, and 0.08~0.13% phosphoric acid aqueous solution as mobile phase B, carry out gradient elution, the ...

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Abstract

The invention relates to a construction method and identification method of UPLC characteristic maps of Pythium spp. The method for constructing the UPLC characteristic map of sage wei, shi wei, Lushan shi wei and North China shi wei comprises the following steps: (1) Precisely weighing the handle shi wei, shi wei, Lushan shi wei, and North China shi wei Wet medicinal materials, prepared the test solution of Shiwei, Shiwei, Lushan Shiwei, and North China Shiwei; Analyzed by high-performance liquid chromatography, the UPLC characteristic spectra of Shiwei, Shiwei, Lushan Shiwei, and North China Shiwei were obtained, and the number of common peaks was determined. The method has good precision, stability and reproducibility, and fully demonstrates the chemical characteristics of S. pedunata, S. wei, Lushan S. wei and North China S. wei. It can better identify S. Shiwei and North China Shiwei medicinal materials.

Description

technical field [0001] The invention belongs to the technical field of medicine, and specifically discloses a method for constructing a UPLC characteristic map of Shiwei, Shiwei, Lushan Shiwei and North China Shiwei and an identification method thereof. Background technique [0002] Shiwei included in the 2015 edition of the Chinese Pharmacopoeia is the dried leaves of Pyrrosia sheareri (Bak.) Ching, Pyrrosia lingua (Thunb.) Farwell or Pyrrosia petiolosa (Christ) Ching . It can be harvested throughout the year, rhizomes and roots removed, and dried in the sun or shade. Pyrrosia petiolosa (Christ) Ching water dragon orthopedic plant, one of the three base sources of traditional Chinese medicine Shiwei. Shiwei is sweet and bitter in taste, slightly cold, and belongs to the lung and bladder meridians. Shi Wei was first recorded in "Shen Nong's Materia Medica", which is listed as a middle grade and has a long history. [0003] At present, identification methods include tradi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 魏梅李国卫杨晓东索彩仙潘礼业孙冬梅陈向东程学仁
Owner GUANGDONG YIFANG PHARMA
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