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A kind of uplc characteristic map construction method and identification method of Pseudostellaria Pseudostellariae medicinal material and Pseudostellariae standard decoction

A technology of characteristic map and construction method, which is applied in the field of UPLC characteristic map construction of Pseudostellaria Pseudostellariae and Radix Pseudostellariae standard decoction.

Active Publication Date: 2022-03-29
GUANGDONG YIFANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few characteristic maps of Pseudostellariae Pseudostellaria reported in the literature, and there is no systematic qualitative analysis of Pseudostellariae Pseudostellariae

Method used

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  • A kind of uplc characteristic map construction method and identification method of Pseudostellaria Pseudostellariae medicinal material and Pseudostellariae standard decoction
  • A kind of uplc characteristic map construction method and identification method of Pseudostellaria Pseudostellariae medicinal material and Pseudostellariae standard decoction
  • A kind of uplc characteristic map construction method and identification method of Pseudostellaria Pseudostellariae medicinal material and Pseudostellariae standard decoction

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Construction method of UPLC characteristic map of Pseudostellaria Pseudostellariae and standard decoction of Pseudostellariae Pseudostellariae.

[0039] experiment method

[0040] Preparation of reference solution

[0041] Take an appropriate amount of Pseudostellaria cyclic peptide B reference substance, weigh it accurately, add methanol to make a solution containing 20ug per 1ml, and obtain it.

[0042] Preparation of Pseudostellaria Pseudostellaria Standard Decoction for Test Solution

[0043] Take 0.2g of Pseudostellaria standard decoction powder, put it in a stoppered Erlenmeyer flask, add 50ml of 70% ethanol precisely, weigh it, and ultrasonically treat it for 30 minutes at a power of 250W and a frequency of 40kHz, let it cool, and then weigh it with Make up the lost weight with 70% ethanol, shake well, and filter; accurately measure 25ml of the filtrate, evaporate to dryness, add 50% methanol to dissolve the residue, transfer them to 10ml measuring bottles, add...

Embodiment 2

[0061] A method for identifying medicinal materials of Radix Pseudostellariae, the steps are as follows:

[0062] (1) Accurately weigh the Pseudostellariae medicinal material to be identified, and prepare the sample solution of the Pseudostellariae Pseudostellariae material to be identified;

[0063] (2) Absorb the sample solution of Pseudostellaria Pseudostellariae to be identified, inject it into an ultra-high performance liquid chromatograph for analysis, and obtain the UPLC spectrum of Pseudostellaria Pseudostellariae to be identified;

[0064] (3) Compare the UPLC characteristic spectrum of Pseudostellariae Pseudostellariae to be identified with the UPLC characteristic spectrum of Pseudostellariae Pseudostellariae constructed; if the UPLC characteristic spectrums of the two are consistent, it is qualified Pseudostellariae Pseudostellariae.

[0065] Preparation of reference solution

[0066] Take an appropriate amount of Pseudostellaria cyclic peptide B reference substanc...

Embodiment 3

[0075] A kind of identification method of Radix Pseudostellariae standard decoction, the steps are as follows:

[0076] (1) Accurately weigh the Pseudostellariae standard decoction powder to be identified, and prepare the sample solution of Pseudostellariae standard decoction to be identified;

[0077] (2) Take the sample solution of Pseudostellaria Pseudostellaria standard decoction to be identified, inject it into the ultra-high performance liquid chromatography for analysis, and obtain the UPLC spectrum of Pseudostellaria Pseudostellaria standard decoction;

[0078] (3) Compare the UPLC characteristic spectrum of Pseudostellaria Pseudostellariae standard decoction to be identified with the UPLC characteristic spectrum of Pseudostellaria Pseudostellariae standard decoction constructed; if the UPLC characteristic spectra of the two are consistent, it is qualified Pseudostellariae standard decoction.

[0079] Preparation of reference solution

[0080] Take an appropriate amou...

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Abstract

The present invention relates to a UPLC characteristic map construction method and identification method of Pseudostellaria Pseudostellariae and Pseudostellaria Pseudostellariae standard decoction. The UPLC characteristic map construction method of Pseudostellariae Pseudostellariae comprises the following steps: (1) Precisely weighing Pseudostellariae Pseudostellariae Radix Pseudostellariae standard decoction powder, prepared Pseudostellariae medicinal material, Pseudostellariae standard decoction solution; UPLC characteristic spectrum of standard decoction. The characteristic spectrum can more comprehensively reflect the characteristics of the medicinal materials of Radix Pseudostellariae and the standard decoction of Radix Radix Pseudostellariae, and the method is accurate, reliable, easy to operate and good in reproducibility. To provide a rapid, accurate and objective method for the identification of Pseudostellaria Pseudostellariae and standard decoction of Pseudostellariae.

Description

technical field [0001] The invention belongs to the technical field of drug analysis, and in particular relates to a construction method and identification method of UPLC characteristic maps of Pseudostellariae medicinal material and Pseudostellariae standard decoction. Background technique [0002] Pseudostellaria heterophylla (Miq.) Pax ex Paxet Hoffm is the dried root of Pseudostellaria heterophylla (Miq.). Excavate when most of the stems and leaves are withered in summer, wash them, remove fibrous roots, put them in boiling water for a while, and then dry them in the sun or directly in the sun. It is a commonly used traditional Chinese medicine in clinic. Beneficial qi and invigorating the spleen, promoting body fluid and nourishing the lungs, used for spleen deficiency, tiredness, loss of appetite, weakness after illness, deficiency of qi and yin, spontaneous sweating and thirst, dryness of the lungs and dry cough. Modern pharmacological studies have shown that Radix P...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/14
CPCG01N30/02G01N30/14G01N2030/146
Inventor 官永河杨小龙罗宇琴李国卫程学仁魏梅孙冬梅陈向东
Owner GUANGDONG YIFANG PHARMA
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