Refined plant polysaccharide and preparation method and application thereof in preparing cosmetics

A technology of plant polysaccharides and total polysaccharides, applied in cosmetics, cosmetic preparations, dressing preparations, etc., can solve the problems of resource waste and low utilization, and achieve the effect of promoting skin repair, proliferation and migration

Active Publication Date: 2019-11-15
上海瞬锋化工科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Butterbur is a very common perennial herb, but its utilization is extremely low, resulting in a great waste of resources

Method used

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  • Refined plant polysaccharide and preparation method and application thereof in preparing cosmetics
  • Refined plant polysaccharide and preparation method and application thereof in preparing cosmetics
  • Refined plant polysaccharide and preparation method and application thereof in preparing cosmetics

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0025] 1. Experimental method

[0026] 1. Preparation of refined plant polysaccharides A and B

[0027] (1) Preparation of total polysaccharide: Grind the dried whole plant of Butterbur, add absolute ethanol and heat reflux for 3 hours to degrease according to the material-to-liquid ratio of 1:5 (kg:L), repeat the degreasing once, filter, and filter the residue according to the material liquid Add distilled water at a ratio of 1:10 (kg:L), heat and extract at 85°C for 12 hours, filter, collect the filtrate, concentrate the filtrate under reduced pressure to an extract, add distilled water according to the ratio of material to liquid 1:5 (kg:L), and stir to fully Dissolve, filter with suction, add 4 times the volume of absolute ethanol to the filtrate, stir evenly, precipitate with alcohol for 12 hours, centrifuge, discard the supernatant, wash with acetone and absolute ethanol in turn, dissolve with an appropriate amount of distilled water, and freeze-dry to obtain total butte...

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Abstract

The invention discloses a refined plant polysaccharide and a preparation method and application thereof in preparing cosmetics. The refined plant polysaccharide is prepared in the following steps thatfirstly, a total polysaccharide of petasites japonicus is prepared through a water extraction alcohol precipitation method, then the total polysaccharide of the petasites japonicus is extracted and dissolved with deionized water, the mixture serves as a sample solution and is loaded in a DEAE-Sepharose Fast Flow chromatographic column and sequentially eluted with a sodium chloride solution with the concentration of 0 mol/L and a sodium chloride solution with the concentration of 0.2 mol/L respectively, and a 0.2 mol/L sodium chloride solution eluant is collected, concentrated, subjected to desalting through a dialysis bag and freeze-dried. The refined plant polysaccharide can promote proliferation and migration of human skin fibroblast cells, has the effect of promoting skin repair, has no obvious irritation to human skin, and has a value for preparing the cosmetics for promoting skin repair.

Description

technical field [0001] The invention belongs to the field of cosmetics and relates to the use of polysaccharides from natural sources in cosmetics, in particular to a refined plant polysaccharide, a preparation method and an application in the preparation of cosmetics. Background technique [0002] It is human nature to love beauty, but with age, people's skin repair ability and skin elasticity will decline. [0003] Human skin repair ability mainly depends on the proliferation speed and migration ability of human skin fibroblasts. As people age, the decline in proliferation speed and migration ability of skin fibroblasts is the direct cause of the decline in skin repair ability. [0004] The elasticity of human skin is mainly related to the content of collagen and elastin. Collagen and elastin are composed of collagen fibers and elastic fibers respectively. Collagen fibers mainly support volume, maintain moisture, and make the skin plump. Elastic fibers mainly enable the s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K8/73A61Q19/00
CPCA61K8/73A61K2800/72A61K2800/84A61Q19/00C08B37/0003
Inventor 洪其昌
Owner 上海瞬锋化工科技有限公司
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