Manufacturing method of urinary albumin test electrode

Abstract

The invention discloses a manufacturing method of a urinary albumin test electrode. The manufacturing method comprises the steps of preparing a PET substrate; manufacturing a graphene working electrode, a reference electrode and a counter electrode; printing high-molecular insulating paint on the PET substrate except an insertion end and a reaction area by adopting a printing method to form an insulating layer; uniformly coating a cross-linking agent and a urinary albumin selective reactant solution on a sample reaction area taking graphene as a working electrode, silver/silver chloride as a reference electrode and carbon as a counter electrode by a spin-coating method, drying at a temperature of 39-42 DEG C, covering the reaction area with a layer of PVC insulating film to form a sample inlet, and thus obtaining the urinary albumin test electrode. The urinary albumin test electrode has the advantages of short consumed time and high working efficiency during detection; in addition, thecontent of the urinary albumin can be detected at proper time, and the difficulty of collecting a 24-hour urine sample during detection in the prior art is avoided.

Description

technical field [0001] The invention relates to the field of medical clinical testing, in particular to a method for making a urine albumin testing electrode. Background technique [0002] Urinary albumin is an important sensitive indicator of early renal disease. It is also the gold standard for non-invasive monitoring of kidney and other vascular diseases caused by diabetes and hypertension. In recent decades, it has become a major public health problem in China and even the world. The incidence of chronic kidney disease is increasing year by year worldwide. There are 130 million patients with chronic kidney disease in my country, following cardiovascular and cerebrovascular diseases, diabetes, Tumor is another major disease that directly threatens human health. [0003] But now in medical clinical testing, enzyme-linked immunoassay, radioimmunoassay, high performance liquid chromatography and immune turbidimetry are mostly used for urine albumin testing. in: [0004] T...

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Problems solved by technology

[0004] The detection time of ELISA is longer; the sensitivity and accuracy of radioimmunoassay are higher, but there is radioactive contamination

[0005] The HPLC process is complex and the operation process is cumbersome

[0006] Immunoscattering turbidimetry is a commonly used method for the determination of urinary albumin in clinical testing. Due to the low content of urinary albumin (normal value less than 20 mg / L), the sensitivity and specificity of immunoscattering turbidimetry cannot be measured. Therefore, the test results cannot be used as the basis for accurate diagnosis of kidney injury, and the test process takes a long time. It takes about 4 to 5 hours from the time the patient takes the specimen to the test result, which cannot meet the convenience and convenience of modern precision medicine. Rapid and accurate diagnosis and monitoring of disease requirements

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