A kind of plant lactobacillus x7022 and its application
A technology of Lactobacillus plantarum and bacterial liquid, applied in the direction of Lactobacillus, application, bacteria, etc., can solve the problems of difficulty in determining the cause of disease, lack of treatment methods and drugs, etc., to alleviate and improve aging memory decline and good aging memory decline , The effect of broad market application value
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Embodiment 1
[0086] Activation of embodiment 1 Lactobacillus plantarum X7022
[0087] Pick a part of the bacterial solution from the glycerol tube of Lactobacillus plantarum X7022, streak it on the MRS solid plate, and incubate at 37°C for 36-48h, inoculate the single colony on the plate into 10mL MRS medium, culture at 37°C for 18h, press 2% of the inoculum was inoculated into 10 mL of MRS culture for activation for 18 hours, the obtained bacterial liquid was centrifuged at 3000 rpm for 10 min, and the obtained bacterial cells were mixed with 10 mL of PBS buffer to obtain the test bacterial liquid.
Embodiment 2
[0088] The preparation of embodiment 2 Lactobacillus plantarum X7022 freeze-dried bacteria powder
[0089] In a sterile operating bench, pick a small amount of bacterial liquid from the glycerin tube of Lactobacillus plantarum X7022, streak it on the MRS solid plate, culture at 37°C for 36-48 hours, and inoculate a single colony on the plate into 10 mL of liquid MRS medium After anaerobic culture at 37°C for 18 hours, they were inoculated in a 10L fermenter containing MRS medium, anaerobically fermented for 24 hours, and then collected by centrifugation at 6000rmp for 10 minutes, washed twice with sterile phosphate buffer (pH=6.4). Centrifuge and mix the resulting sludge with skimmed milk powder containing 18%-22%, 4-9% trehalose, 2-4% sodium glycinate, 1-5% glycerin and 0.1-1% cysteine hydrochloride The sterilized lyoprotectant is mixed with emulsified, bacteria sludge and lyoprotectant in different weight ratios. After the bacteria slime and protective agent are fully sus...
Embodiment 3
[0090] Example 3 Intestinal survivability experiment of Lactobacillus plantarum X7022
[0091] 1. Preparation of intragastric bacteria solution
[0092] Weigh part of the Lactobacillus plantarum X7022 bacterial powder in Example 2 and store it at 4°C, set according to Table 2, and dilute it with sterile physiological saline to the required bacterial concentration to obtain the bacterial liquid required for CFDA-SE fluorescent dyeing and labeling .
[0093] Table 2 Settings of intragastric solution
[0094] Gastric solution name Bacteria concentration in intragastric solution (CFU / mL) Remark X7022 high concentration 1×10 9
Experimental group A X7022 low concentration 1×10 6
Experimental group B control group 0 Experimental group C
[0095] First, centrifuge to collect the bacteria, use 1mL staining buffer to suspend the bacteria in a 15mL centrifuge tube, adjust the bacterial concentration to 1×10 9 CFU / mL and 1×10 6CFU / mL, di...
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