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Lactobacillus fermentum TY-G04 with bowel relaxing effect and application of lactobacillus fermentum TY-G04

A technology of Lactobacillus fermentum and laxative, applied in the field of microorganisms, can solve the problems of difficult treatment, easy repetition, easy recurrence, etc., and achieve the effect of good digestive tract resistance and intestinal colonization ability

Active Publication Date: 2022-02-08
CHONGQING TIANYOU DAIRY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the human body is in a state of constipation for a long time, the toxins in the body cannot be removed in time, which will cause the toxins in the body to be repeatedly absorbed and utilized by the intestinal tract and enter the blood circulation, causing harm, disease, and even cancer to the internal organs
Clinically, drugs or surgery are usually used to treat constipation. Although drug treatment has a certain effect, long-term drug resistance, dependence and other complications may occur in the body, such as drug dependence, nausea and severe diarrhea; surgical treatment is immediate, but Treat the symptoms but not the root cause, if you don’t pay attention, it’s easy to relapse
It can be seen that the current clinical treatment for constipation symptoms still has the characteristics of difficult treatment and easy recurrence.

Method used

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  • Lactobacillus fermentum TY-G04 with bowel relaxing effect and application of lactobacillus fermentum TY-G04
  • Lactobacillus fermentum TY-G04 with bowel relaxing effect and application of lactobacillus fermentum TY-G04
  • Lactobacillus fermentum TY-G04 with bowel relaxing effect and application of lactobacillus fermentum TY-G04

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Isolation, purification and identification of TY-G04

[0050] 1. Experimental materials

[0051] Collect naturally fermented yak yoghurt from the herdsman's house in Hongyuan County, Aba Tibetan and Qiang Autonomous Prefecture, Sichuan Province: take the yak yoghurt made by the herdsman's house with a sterile spoon, and put it into 15mL sterile spinner containing appropriate amount of sterile calcium carbonate and soluble starch. Cover the centrifuge tube (calcium carbonate: soluble starch ratio is 1: 1), screw the cap tightly after stirring evenly, then put it into a refrigerator, and transport it back to the laboratory for the purification and separation of lactic acid bacteria immediately.

[0052] 2. Separation and purification of TY-G04

[0053] Under sterile conditions, pipette 1mL sample into 9mL sterile normal saline, vortex to mix, that is 10 -1 sample diluent, and then serially diluted 10 times to 10 -7 , select 10 -5 、10 -6 、10 -7 100 μL of the diluted ...

Embodiment 2

[0064] Determination of TY-G04 Gastrointestinal Fluid Tolerance and Cell Adhesion

[0065] 1. Experimental materials

[0066] Lactobacillus fermentum TY-G04 with laxative effect: isolated from naturally fermented yak yoghurt from herdsmen in Hongyuan, Sichuan, and preserved in the General Microbiology Center of China Committee for the Collection of Microbial Cultures, numbered CGMCC No.23752.

[0067] 2. Determination of TY-G04 tolerance to pH3.0 artificial gastric juice

[0068] TY-G04 was inoculated in MRS liquid medium at 2% inoculation amount, cultured at 37°C for 18 hours, and 10 mL was centrifuged at 4000 r / min for 10 minutes to collect bacterial precipitates, washed twice with sterile normal saline, and resuspended In an equal volume of sterile saline, it is a bacterial suspension. Mix the prepared bacterial suspension with artificial gastric juice (0.2% NaCl, 0.35% pepsin 1:10000, adjust pH=3.0 with 1mol / L HCl, filter and sterilize for later use) in a ratio of 1:9, a...

Embodiment 3

[0083]Improvement effect of TY-G04 on constipation

[0084] 1. Experimental materials

[0085] Lactobacillus fermentum TY-G04 with laxative effect: isolated from naturally fermented yak yoghurt from herdsmen in Hongyuan, Sichuan, and preserved in the General Microbiology Center of China Committee for the Collection of Microbial Cultures, numbered CGMCC No.23752.

[0086] 2. Animal verification experiments

[0087] 2.1. Grouping and handling of experimental animals

[0088] Select 7-week-old BALB / c male mice (20g-25g) and raise them in a standardized laboratory at room temperature 25±2°C, relative humidity 50±5%, 12h light / 12h dark, and start the experiment after one week of adaptive feeding . After the adaptation period, the mice were randomly divided into a normal group, a constipation group, and a TY-G04 group, with 10 mice in each group.

[0089] The experimental period was 18 days, and 1-14 days was the TY-G04 intervention period. The normal group and the constipation ...

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Abstract

The invention relates to lactobacillus fermentum TY-G04 with an effect of relaxing bowel, the strain is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms, the preservation date is November 8, 2021, and the preservation number is CGMCC No.23752. Animal verification experiments confirm that the lactobacillus fermentum TY-G04 with the bowel relaxing effect can improve constipation, the improvement mode is that the release of 5-HT is promoted by generating metabolites acetic acid and butyric acid, and the 5-HT transmits signals to cerebral neurons by stimulating secretion of gastrointestinal regulatory peptides, so that intestinal peristalsis reflex is triggered, the bowel movement is accelerated, so that the effect of improving constipation is finally achieved. According to the method, the interaction between the brain axis and the intestinal axis of the constipation patient is preliminarily explored, and a wider thought is provided for diagnosis and treatment of current constipation.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a strain of Lactobacillus fermentum TY-G04 with the effect of moistening the intestines and laxative and its application. Background technique [0002] The intestinal tract is the first line of defense to protect human health, and it is also the body's scavenger. 99% of the nutrients in the human body are absorbed by the intestinal tract, and more than 80% of the toxins are excreted through the intestinal tract. If there is a disease in the intestinal tract, it will have a certain impact on human health. [0003] Constipation is one of the most common intestinal diseases. Its main manifestations are decreased frequency of defecation, difficulty in defecation, induration of feces, less volume, anal discomfort, bloating and blood in the stool during defecation. If the human body is in a state of constipation for a long time, the toxins in the body cannot be removed in time, which...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P1/00A61P1/10A23L33/135C12R1/225
CPCA61K35/747A61P1/00A61P1/10A23L33/135A23V2002/00A23V2400/143A23V2200/3204Y02A50/30A61K2035/115C12R2001/225C12N1/205
Inventor 唐甜张凤蒋源渊张真王静祁腾索化夷李婉竹邓雅丹
Owner CHONGQING TIANYOU DAIRY CO LTD