Lactobacillus plantarum X7022 and application thereof
A technology of Lactobacillus plantarum, bacterial liquid, applied in the directions of Lactobacillus, application, bacteria, etc., can solve the problem of difficulty in determining the cause, lack of treatment methods and drugs, etc., to alleviate and improve aging memory decline, good aging memory decline , the effect of good intestinal colonization ability
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Embodiment 1
[0086] Example 1 Activation of Lactobacillus plantarum X7022
[0087] Pick out part of the bacterial liquid from the glycerol tube of Lactobacillus plantarum X7022, streak it on the MRS solid plate, cultivate at 37°C for 36-48h, pick a single colony on the plate and inoculate it in 10mL MRS medium, incubate at 37°C for 18h, press 2% of the inoculum was added to 10 mL of MRS culture for activation for 18 h, the obtained bacterial solution was centrifuged at 3000 rmp for 10 min, and the obtained bacterial cells were mixed with 10 mL of PBS buffer to obtain the test bacterial solution.
Embodiment 2
[0088] The preparation of embodiment 2 Lactobacillus plantarum X7022 freeze-dried bacteria powder
[0089] In a sterile operating table, pick out a small amount of bacterial liquid from the glycerol tube of Lactobacillus plantarum X7022, streak it on the MRS solid plate, cultivate at 37°C for 36-48 hours, pick a single colony on the plate and inoculate it in 10 mL of liquid MRS medium , after anaerobic culture at 37°C for 18h, inoculated into a 10L fermenter containing MRS medium, anaerobic fermentation for 24h, and then centrifuged at 6000rmp for 10min to collect bacteria, and washed twice with sterile phosphate buffer (pH=6.4). Centrifuge and mix the resulting mash with skim milk powder containing 18%-22%, 4-9% trehalose, 2-4% sodium glycinate, 1-5% glycerol and 0.1-1% cysteine hydrochloride The sterilized lyophilized protective agent is mixed and emulsified, and the bacterial puree and the lyophilized protective agent are in different weight ratios. After the bacteria sl...
Embodiment 3
[0090] Example 3 Intestinal viability experiment of Lactobacillus plantarum X7022
[0091] 1. Preparation of gavage bacterial solution
[0092] Weigh part of Lactobacillus plantarum X7022 bacteria powder in Example 2 and store at 4°C, set according to Table 2 respectively, dilute to required bacterial concentration with sterile physiological saline, obtain the required bacterial liquid for CFDA-SE fluorescent staining marking .
[0093] Table 2 Gavage settings
[0094] Gastric fluid name Bacterial concentration in gavage fluid (CFU / mL) Remark X7022 high concentration 1×10 9
Experimental group A X7022 low concentration 1×10 6
Experimental group B control group 0 Experimental group C
[0095] First, the cells were collected by centrifugation, and the cells were suspended in a 15 mL centrifuge tube with 1 mL of staining buffer, and the bacterial concentration was adjusted to 1×10 9 CFU / mL and 1×10 6CFU / mL, dissolve the CFDA-SE f...
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