Method for inhibiting iron-producing carrier bacteria and preventing and treating plant diseases caused by iron-producing carrier bacteria

A plant disease and siderophore technology, applied in the field of agricultural microorganisms, to achieve the effect of inhibiting growth and inhibiting effect

Active Publication Date: 2019-12-03
INST OF AGRI ENVIRONMENT & RESOURCES YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for inhibiting siderophore bacteria and preventing and treating plant diseases caused by siderophore bacteria in order to overcome the problem of rarely inhibiting siderophore bacteria in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] This example is used to illustrate the results of morphological identification and molecular genetic classification identification of the strains involved in the present invention.

[0033] Pseudomonas putida TZT-058 was isolated from a diseased plant of Paris polyphyllavar.yunnanensis (Franch) Hand (Trilliaceae) in Huangshan Town, Yulong Naxi Autonomous County, Lijiang City, Yunnan Province, China. It is deposited in the General Microorganism Center of China Microbiological Culture Collection Management Committee, and the preservation number is CGMCCNo.17926.

[0034] The colony of Pseudomonas putida TZT-058 on the plate medium (beef extract 3g, peptone 10g, sodium chloride 5g, agar about 15g, distilled water 1000mL, pH6.8-7) is round, milky white, opaque, and the surface of the colony is Glossy, distributed on the surface of the culture medium; magnified by scanning electron microscope 21000 times, it is rod-shaped, long rod-shaped bacteria, 2.26 μm long and 0.71 μm w...

Embodiment 2

[0036] This example is used to illustrate the siderophore-producing performance of the strains involved in the present invention.

[0037] (1) Test tube culture

[0038] Inoculate the Pseudomonas putida TZT-058 strain on the solid medium slope, and cultivate it at 30°C for 72 hours to obtain the test tube species; the formula of the solid medium is: beef extract 3g, peptone 10g, sodium chloride 5g, Agar about 15g, distilled water 1000mL, pH6.8-7;

[0039] (2) Solid plate seed culture

[0040] Cultured on plate culture medium, the method is as follows:

[0041] 1. prepare the iron-free solid flat plate seed medium, mix the components and the content thereof to form the solid flat plate seed medium according to the iron-free solid flat plate seed medium formula, and the formula of the iron-free solid flat plate seed medium is: beef extract 3g , peptone 10g, sodium chloride 5g, agar about 15g, 8-hydroxyquinoline 0.75g, deionized water 1000mL, pH6.8-7;

[0042] ②Put 200ml of the...

Embodiment 3

[0050] This example is used to illustrate the preliminary identification of the chemical structure of siderophores produced by the strains involved in the present invention.

[0051] The identification method is as follows:

[0052] ① Identification of hydroxamates siderophore

[0053] The ferric chloride detection method (FeCl 3 test) identification: add 1mL 2% FeCl to 1mL culture medium 3 solution, the appearance of red or purple indicates that the test sample contains siderophore substances. Detect with an ultraviolet spectrophotometer, if an absorption peak appears between 420nm-450nm, it indicates that the chelating substance is a hydroxamic acid type siderophore. Tetrazole test: Add 2 drops of 2mol / L NaOH and 1mL of the 3mg / L siderophore solution prepared above to a small amount of tetrazolium salt. If it turns dark red immediately, it indicates the presence of hydroxamic acid siderophore.

[0054] ② Identification of catecholate siderophore

[0055] Identification...

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PUM

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Abstract

The invention relates to the field of agricultural microorganisms, and discloses a method for inhibiting iron-producing carrier bacteria and preventing and treating plant diseases caused by the iron-producing carrier bacteria. The disclosed method for inhibiting the iron-producing carrier bacteria comprises the step that an iron ion chelating agent is in contact with the iron-producing carrier bacteria. The disclosed method for preventing and treating the plant diseases caused by the iron-producing carrier bacteria comprises the step that the iron ion chelating agent is applied to plants. According to the method, the growth of the iron-producing carrier bacteria can be effectively inhibited, and the plant diseases caused by the iron-producing carrier bacteria are prevented and treated.

Description

technical field [0001] The invention relates to the field of agricultural microorganisms, in particular to a method for inhibiting siderophore-producing bacteria and preventing plant diseases caused by siderophore-producing bacteria. Background technique [0002] The content of iron in the earth's crust and soil is very rich. At the same time, as one of the essential nutrients for almost all organisms on the earth, iron plays an important role in various functional metabolic activities and enzymatic reactions; but it is neutral or alkaline in nature. Under aerobic conditions, iron is mainly in the form of insoluble Fe 3+ Existing in the form of compounds, the content of soluble iron is relatively scarce, and the bioavailability is extremely low. In addition, iron deficiency in plants is common in agricultural production, which has a great impact on the biomass production of agricultural and forestry crops and the systemic resistance of plants. Therefore, in order to adapt ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N37/44A01P1/00A01P3/00A01P21/00C12N1/20C12R1/40
CPCA01N37/44C12N1/205C12R2001/40
Inventor 何翔张庆杨佩文李铭刚朱红业倪明杨群辉杨明英
Owner INST OF AGRI ENVIRONMENT & RESOURCES YUNNAN ACAD OF AGRI SCI
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