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Composition for establishing chronic kidney disease model and application thereof

A technology of chronic kidney disease and composition, which is applied in the field of disease models, can solve the problems that chronic kidney disease models have not been reported, and achieve the effect of increasing the expression of renal fibrosis-related indicators, reducing damage, and weakening side effects

Active Publication Date: 2019-12-03
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no reports at home and abroad on the research on the combination of ochratoxin A and other drugs to induce chronic kidney disease models

Method used

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  • Composition for establishing chronic kidney disease model and application thereof
  • Composition for establishing chronic kidney disease model and application thereof
  • Composition for establishing chronic kidney disease model and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] This example is the preparation of a preferred experimental animal model of chronic kidney disease, and the experimental animals are mice.

[0043] 1. Materials and Methods

[0044] 1.1 Main materials and reagents

[0045] Seventy SPF female C57BL / 6 mice aged 6-8 weeks were purchased from Yangzhou University. The initial body weight was 16.25±0.25g. After the mice were adaptively fed for one week in sterile cages (7 groups, 10 mice / group), the ambient temperature was kept at 22±2°C and the relative humidity was 45%-60% for 12 hours. The light alternates day and night. Food and water were provided ad libitum throughout the experiment, the litter was changed every 3 days, the weight of the mice was recorded every day, and the abnormal symptoms of the mice were monitored. All experiments were managed in accordance with the Animal Experimental Animal Management Regulations of Nanjing Agricultural University.

[0046] Ochratoxin A (OTA) was purchased from Sigma (USA), ...

Embodiment 2

[0084] This example is the preparation of a preferred in vitro cellular chronic kidney disease model, and the cells used are human renal tubular epithelial cells.

[0085] 1. Materials and Methods

[0086] 1.1 Main materials and reagents

[0087]The non-contaminated human renal cortex proximal tubule epithelial cell line HK-2 was purchased from Shanghai Gefan Biotechnology Co., Ltd., and the cells were incubated in 1640 (Invitrogen, USA) containing 10% inactivated fetal bovine serum and 1% penicillin-streptomycin. ) culture medium, the temperature in the incubator is 37° C., the carbon dioxide concentration is 5%, and the pH value is 7.2-7.4. RPMI-1640 medium and fetal bovine serum were purchased from Gibco (Invitrogen) Shanghai Trading Co., Ltd. The penicillin-streptomycin mixture was purchased from Suo Laibao Biotechnology Co., Ltd. Prestained protein markers were purchased from Thermo Fisher, Germany. β-actin primary antibody and anti-rabbit secondary antibody were pu...

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Abstract

The invention relates to a composition for establishing a chronic kidney disease model and a method thereof and an application of the composition and the chronic kidney disease model in screening medicines for treating chronic kidney diseases. The composition comprises ochratoxin A and cyclosporin A. According to the invention, an optimal concentration combination is screened out by using the renal toxicity of ochratoxin A and cyclosporin A to establish the chronic kidney disease model; 0.25 mg / kg.d <-1> ochratoxin A given to the mouse every day and 20 mg / kg.d <-1> cyclosporin A (or 0.5 mu g / mL ochratoxin A and 6 mu g / mL cyclosporin A in vitro) given to the mouse every other day are jointly, so that the kidney function is successfully influenced; the chronic kidney disease model is established, the change process is similar to the change process of the chronic kidney disease formed by singly administrating a high dose of 30mg / kg.d <-1> cyclosporin A every day; and ochratoxin A is used,so that the dosage of cyclosporin A is reduced, the side effects on the heart and the liver are remarkably reduced, the side effects caused by high-concentration cyclosporin A are effectively relieved, the model construction cost is reduced, and a research basis is provided for screening of related drugs.

Description

technical field [0001] The present invention relates to the technical field of disease models, in particular to a composition for establishing a chronic kidney disease model, a method for establishing a chronic kidney disease model, and the use of the composition and the chronic kidney disease model in screening drugs for the treatment of chronic kidney disease Application, specifically, the composition is the combined use of ochratoxin A and cyclosporine A. Background technique [0002] Chronic kidney disease occurs due to various internal and external factors leading to abnormal structure or function of the kidneys. The incidence of chronic kidney disease in adults is 8%-12%. The incidence of chronic kidney disease is characterized by "three highs" and "three lows": high incidence, high prevalence of associated cardiovascular disease, and high mortality; low awareness rate of chronic kidney disease in the whole society, low prevention and treatment rate, and associated T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/13A61K31/366
CPCA61K38/13A61K31/366A61K2300/00
Inventor 黄克和后丽丽乐冠男林籽满甘芳钱刚
Owner NANJING AGRICULTURAL UNIVERSITY
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