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Preparation method of DNA walker and application of DNA walker in sensing analysis

A walker and DNA chain technology, applied in the direction of material analysis through electromagnetic means, analysis of materials through chemical reactions, analysis of materials, etc., can solve problems such as poor conductivity, and achieve the effect of simplifying human operations

Inactive Publication Date: 2019-12-06
UNIV OF JINAN
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the presence of cellulose matrix also brings some difficulties, such as poor electrical conductivity

Method used

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  • Preparation method of DNA walker and application of DNA walker in sensing analysis
  • Preparation method of DNA walker and application of DNA walker in sensing analysis
  • Preparation method of DNA walker and application of DNA walker in sensing analysis

Examples

Experimental program
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Effect test

Embodiment 1

[0037] The preparation method of the paper-based electrochemical luminescence sensing device is as follows:

[0038] (1) Use Adobe illustrator CS4 software to design on the computer as attached figure 1 The hydrophobic wax printing pattern of the paper chip is shown, and the color of the wax printing pattern is black. The paper-based device includes five wax printing areas respectively A, B, C, D and E, where A is the hollow channel unit, and B is the Multi-channel unit, C is the hydrophilic unit, D is the working unit, E is the corresponding unit, the size of A and B is 40×50 mm, the size of C is 20×40 mm, and the size of D and E is 20×24 mm , the hollow channel on A is a circle with a diameter of 10 mm, the width of the triple channel on B is 4 mm, the area of ​​the hydrophilic area on C is 38 × 18 mm, and the area of ​​the hydrophilic area on D and E is 10 mm round;

[0039] (2) Print the hydrophobic pattern designed in step (1) onto the paper chip with a Fuji Xerox wax p...

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Abstract

The invention discloses a preparation method of a DNA walker and an application of the DNA walker in sensing analysis. A hydrophobic area, a hydrophilic area, a hollow channel and a fluid channel netare prepared on paper by utilizing wax printing and laser cutting machine technologies to realize automatic cleaning and signal acquisition of a paper electrode. The method comprises steps that streptavidin and a DNA chain modified with biotin are assembled, the multi-foot DNA walker is constructed, the DNA walker is pushed to move on a surface of the electrode by virtue of a chain substitution reaction, so a DNA-platinum-copper nano composite material is fixed on the surface of the electrode, and a luminol light-emitting signal is amplified by virtue of the extremely good catalytic action ofthe platinum-copper nano material on hydrogen peroxide, so ultra-sensitive detection of a to-be-detected object is realized.

Description

technical field [0001] The invention relates to the technical field of electrochemiluminescence sensing analysis and detection, more specifically the preparation of a DNA walker and its application in electrochemical sensing analysis. Background technique [0002] Protein plays a vital role in most life activities such as metabolism, heredity and immunity. In particular, streptavidin, a protein isolated from Streptomyces, can survive and maintain activity under conditions such as high temperature, extreme pH, and denaturing agents, which is of great significance to organisms. At present, researchers have studied a variety of streptavidin detection techniques. For example, Li et al. successfully combined binding-induced DNA strand displacement and fluorescence detection to detect streptavidin. In addition, Kizek's research group proposed a square wave voltammetry method based on carbon paste electrodes for the determination of streptavidin. Although these methods have high...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/38G01N27/327G01N21/76
CPCG01N21/76G01N27/3278G01N27/38
Inventor 于京华黄煜真李丽张彦赵珮妮葛慎光
Owner UNIV OF JINAN
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