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Application of Cotton ghmads45-d09 Gene in Promoting Plant Flowering

A technology of ghmads45-d09 and 1.ghmads45-d09 is applied in the application field of GhMADS45-D09 gene in promoting plant flowering, which can solve the problems of difficult research, complex genetic basis, low heritability and the like, and achieve the effect of advancing flowering time

Active Publication Date: 2022-01-25
INST OF COTTON RES CHINESE ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The main breeding target traits of upland cotton include traits related to prematurity, fiber quality, yield traits, and traits related to resistance to diseases and insect pests and adversity. Most of these traits are complex quantitative traits that are easily affected by the environment and have relatively low heritability. , the genetic basis is complex and difficult to study

Method used

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  • Application of Cotton ghmads45-d09 Gene in Promoting Plant Flowering
  • Application of Cotton ghmads45-d09 Gene in Promoting Plant Flowering
  • Application of Cotton ghmads45-d09 Gene in Promoting Plant Flowering

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Embodiment 1

[0026] 1. Cotton material

[0027] The cotton materials selected in this example are Upland cotton 50, Zhongmian Institute 74, Guoxin cotton No. 11, and Bomian No. 1, which were planted in the State Key Laboratory of Cotton Biology, Cotton Research Institute, Chinese Academy of Agricultural Sciences (Anyang Baibi) , the management measures are normal field management.

[0028] 2. Reagents and consumables

[0029] 2.1 Enzymes and kits: GXL DNA Polymerase High-Fidelity Enzyme, Gel Recovery Kit, and PCR Product Purification Kit were purchased from Takara Company; RNA Reverse Transcription Kit, KOD FX Neo Enzyme (Code.No.KFX-201) were purchased from Toyobo Company; The Ultra One Step Cloning Kit kit was purchased from Vazyme Company; a small amount of plasmid extraction kit was purchased from Magen Company; restriction endonucleases (BamH I, Sac I) were purchased from NEB Company; DNAMarker III and plant total RNA extraction kits were purchased from TIANGEN Company; fluoresce...

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Abstract

The invention discloses cotton GhMADS45‑D09 The application of genes in promoting plant flowering belongs to the technical field of plant genetic engineering. GhMADS45‑D09 The gene has the nucleotide sequence shown in SEQ ID NO:1 and can encode the amino acid sequence shown in SEQ ID NO:2. The transgene obtained by the present invention through transgenic technology GhMADS45‑ D09 The Arabidopsis plants of the gene make the flowering time of Arabidopsis significantly earlier. Further through Silent Cotton GhMADS45‑D09 gene, it turns out that GhMADS45‑D09 Gene plays key role in promoting flowering in cotton. The invention provides favorable gene resources for short-season cotton cultivation.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, and in particular relates to the application of GhMADS45-D09 gene in promoting flowering of plants. Background technique [0002] The main breeding target traits of upland cotton include traits related to prematurity, fiber quality, yield traits, and traits related to resistance to diseases and insect pests and adversity. Most of these traits are complex quantitative traits that are easily affected by the environment and have relatively low heritability. , the genetic basis is complex and difficult to study. Appropriate growth period (maturity) plays an irreplaceable role in high quality, high yield and suitable machine harvesting of cotton. The discovery of early-maturing genes that play an important role in the maturity period of upland cotton will play a positive role in promoting the selection of fine varieties of upland cotton that have the characteristics of early maturit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/00A01H6/20
CPCC07K14/415C12N15/827
Inventor 喻树迅程帅帅马亮王寒涛魏恒玲芦建华李林
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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