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Saccharomycetes for producing cellulase at low temperature and screening method thereof

A technology of cellulase and yeast, which is applied in the field of microbiology, can solve the problems that the screening method has not been reported, and achieve the effect of improving the micro-ecosystem, enhancing air permeability, and low cost

Active Publication Date: 2019-12-13
LIAONING ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0005] In conclusion, a strain of yeast producing cellulase at low temperature and its screening method have not been reported yet.

Method used

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  • Saccharomycetes for producing cellulase at low temperature and screening method thereof
  • Saccharomycetes for producing cellulase at low temperature and screening method thereof
  • Saccharomycetes for producing cellulase at low temperature and screening method thereof

Examples

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Embodiment example

[0066] CK: 1 ton of corn stover adjusts the water content to 50%.

[0067] H4: Add bacteria H44kg on the basis of CK.

[0068] Composting time: 2019.3.13-2019.4.25.

[0069] Compost location: Shenyang.

[0070] Specific process: For each pile of 1000kg of compost, pile the corn stalks neatly parallel to the ground. When every 20cm rises, sprinkle a little inoculant on the surface of the compost. At the same time, step on it tightly, keep the pile height 1.5, and cover the outer surface with agricultural plastic film , A total of 45 days of accumulation.

[0071] Indicators: Determine the temperature of straw composting and the lignin and cellulose content of straw in different periods.

[0072] The results showed that under the condition of an average temperature of 6℃ in spring, by adding low-temperature cellulase-producing yeast, the average temperature of straw compost reached 46.7℃, which was 48.6% higher than the control, lignin was 24.2% lower than the control, and the cellulose c...

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Abstract

The invention relates to saccharomycetes for producing cellulase at low temperature and a screening method of the saccharomycetes, the saccharomycetes comprise saccharomycetes, a preservation institution is China General Microbiological Culture Collection Center, a preservation number is CGMCC No.18002, and a preservation date is June 19th, 2019. The efficient low-temperature cellulase saccharomycetes are separated and screened from soil in different low-temperature regions, and the saccharomycetes are biologically characterized in that the saccharomycetes are milk-white round bacterial colonies on a potato glucose culture medium, are slightly dry in surfaces, thick in bacterial colonies and easy to pick up, and have wine fragrance, which present typical saccharomycetes bacterial colonies;and the influence of conditions such as temperature, carbon source, nitrogen source, pH and the like on cellulase is studied. By applying the method to straw returning in a northeast low-temperaturecold region, the influence of straw incineration in the northeast region on the environment is reduced, and a solid foundation is laid for agricultural sustainable development.

Description

Technical field [0001] The invention relates to a low-temperature cellulase-producing yeast and a screening method thereof, belonging to the field of microbiology. Background technique [0002] Low-temperature microorganisms refer to groups of microorganisms that have the ability to grow and reproduce in a low-temperature environment, and they grow and reproduce in various low-temperature environments. According to the selection of the growth temperature of microorganisms, low-temperature microorganisms are divided into two categories, psychrophilic and psychrophilic microorganisms. Among them, psychrophilic microorganisms can grow and reproduce, and the optimum temperature is about 15℃, which can survive. The temperature is about 20℃; cold-tolerant microorganisms are microorganisms whose survival temperature is about 0℃ and the optimal temperature for growth and reproduction is about 20℃. There are very rich microbial resources and low-temperature microbial resources in the bio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12N9/42C12Q1/34C12Q1/04C12R1/645
CPCC12N9/2437C12Y302/01004C12Q1/34C12Q1/04G01N2333/924G01N2333/39C12R2001/645C12N1/145
Inventor 何志刚隋国民宋艳雨刘惠屿刘艳
Owner LIAONING ACAD OF AGRI SCI
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