Application of Taiwanin E in preparation of herbicides
A herbicide and weed technology, which is applied in the field of pesticide research and development, can solve the problem that the research on the herbicidal activity of aryl naphthalene lignan compounds has not been reported, and achieves a significant fresh weight inhibition rate, high environmental protection, and good inhibition of root growth. Effect
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Embodiment 1
[0029] Example 1 Separation of compound Taiwanin E
[0030] After the stem bark of Taiwania chinensis was dried and crushed, it was extracted with methanol and concentrated to obtain a methanol extract. After the extract was dissolved in methanol and suspended in water, it was extracted with petroleum ether and ethyl acetate to obtain an ethyl acetate layer extract.
[0031] The ethyl acetate extract was first subjected to silica gel column chromatography using a chloroform / methanol system, and then combined by TLC. Combined with activity tracking, the active part is subjected to silica gel column chromatography with petroleum ether / acetone and petroleum ether / ethyl acetate, and then separated by Sephadex LH-20. After purification, Taiwanin E can be obtained.
Embodiment 2
[0032] Example 2 Herbicidal activity of Taiwannin E
[0033] The herbicidal activity of Taiwanin E on different kinds of weeds was determined by the small cup method. The results are shown in Table 1.
[0034] Table 1 The herbicidal activity of Taiwanin E (50mg / L, 7d)
[0035] Weed species Root length inhibition rate (%) Fresh weight inhibition rate (%) Red thistle (Ageratum conyzoides L.) 100.00±0.00 70.58±0.77 Lotus Bean Grass (Drymaria diandra Blume) 100.00±0.00 71.59±0.37 Solanum nigrum L. 100.00±0.00 63.37±1.44 Portulaca (Portulaca oleracea L.) 94.36±0.47 66.56±2.77 Duckweed (Lemna minor L.) 86.39±0.69 83.71±1.63 Amaranthus retroflexus (Amaranthus retroflexus L.) 74.56±0.40 50.24±1.95 Mikania micrantha Kunth 70.66±2.90 58.72±2.78 Eleusine indica (L.) Gaertn) 70.49±1.11 42.02±2.05 Clover Bidens (Bidens pilosa L.) 55.63±1.84 39.51±2.33 Bald Barnyard (Echinochloa colonum (L.) Link) 55.73±2.74 44.96±0.85 Sonchus arvensis L. 51.97±3.15 45.37±1.03 Celosia argentea L. (C...
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