Fertilizing and molluscicidal double-effect organic fertilizer molluscicide as well as preparation method and application thereof
A technology of organic fertilizer and molluscicide, applied in the field of agricultural pesticides, can solve problems such as soil and water pollution, water eutrophication aggravated, etc., to reduce the harm of schistosomiasis infection, facilitate production, and have obvious social and ecological effects. Effect
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Embodiment 1
[0042] Example 1 Extraction of quaternary ammonium triphenanthridine alkaloids see detection
[0043] Extraction of quaternary ammonium triphenanthridine alkaloids:
[0044] (1) The quaternary amine triphenanthridine alkaloids can come from plants such as Boluohui, celandine, blood water grass, etc. In this embodiment, taking Boluohui as an example to describe in detail, wash it with clean water and dry it, and ultrasonically smash.
[0045] (2) Extract the plant powder with acid water (hydrochloric acid, pH 1.5) in a water bath at 85° C. for 3 hours according to the ratio of M:V (mass volume ratio) = 1:10. Then use vacuum filtration to collect the filtrate. Repeat three times, combine the filtrate, add high-concentration sodium hydroxide solution, adjust the pH to 10, let it stand overnight, centrifuge to collect the precipitate, dry and freeze-dry in vacuum to obtain a brown yellow powder, that is, the total alkali of Boluohui.
[0046] (3) Separation of benzophenanthridi...
Embodiment 2
[0047] Example 2 Detection of Molluscicidal Effect of Quaternary Ammonium Triphenanthridine Alkaloids
[0048]Taking niclosamide ethanolamine salt, spiroxamine, as the control, the quaternary ammonium benzophenanthridine alkaloids were prepared with different concentrations of solutions with water as the solvent, and the indoor immersion method recommended by "WTO" was used to explore the quaternary ammonium benzophenanthridine alkaloids. Molluscicidal effect of phenanthridine alkaloids. The mollusc killing effect is shown in the following table 1 and Figure 8 .
[0049] Table 1 Molluscicidal effect of quaternary ammonium triphenanthridine alkaloids
[0050]
[0051] It can be known from Table 1 that when the concentration of the quaternary ammonium triphenanthridine alkaloids extracted from the Boluohuiguo folder was 7.5 mg / L, the lethality of Oncomelania snails reached 100% after treatment for 72 hours; when the concentration was 2.5 mg / L , after 120 hours of treatmen...
Embodiment 3
[0053] Example 3 Indoor molluscicide test and effect evaluation of Boluohui quaternary amine benzophenanthridine alkaloid and potassium peptide fertilizer compound molluscicide
[0054] For the formula of the fertilizer-type water agent molluscicide used in this example, see the potassium peptide fertilizer formula given in Example 4.
[0055] Indoor molluscicide efficacy test of molluscicides: carried out in accordance with "Efficacy Test Methods and Evaluation of Molluscicides for Pesticide Registration" NY / T1617-2008.
[0056] Test conditions: laboratory temperature is 26±1°C, relative humidity is 65±5%.
[0057] Oncomelania hupensis to be tested: Oncomelania hupensis collected from the field in Hubei Province. Negative snails with 6-8 spins were isolated in the laboratory.
[0058] Instruments and equipment: electronic balance, incubator, measuring cylinder, weighing bottle, beaker (300ml), PH tester, sprayer, stirrer, petri dish (diameter 9cm), snail bag, copper hammer, ...
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