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Ornithine contained short chain polypeptide and application thereof

An ornithine and short peptide technology, which is applied to ornithine-containing short peptides and their application fields, can solve the problems of raw material nutrient loss, required equipment, material nutrient loss, etc., and achieves a good inhibitory effect.

Active Publication Date: 2019-12-20
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, each method has limitations, either because the nutrition of the raw material is lost, or because expensive equipment is required, etc.
For example, the structure of aflatoxin is very stable and can be decomposed at 270°C. If heat treatment is used, the higher the temperature and the longer the heating time, the higher the removal rate of aflatoxin. However, it is obvious that the heating method will easily cause the nutrient loss of the material, and Equipment requiring high voltage resistance

Method used

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  • Ornithine contained short chain polypeptide and application thereof
  • Ornithine contained short chain polypeptide and application thereof
  • Ornithine contained short chain polypeptide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The embodiment of the present invention provides a short peptide containing ornithine, which is isolated for the first time from the fermentation broth of Bacillus megaterium, specifically as follows:

[0025] Microorganisms used:

[0026] Aspergillus flavus A.flavus NRRL 3357: cultivated in PDA medium (200g potato extract, 20g glucose, 20g agar, 1L water).

[0027] Bacillus megaterium (CGMCC7086, preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee): cultured in beef extract peptone medium (beef extract 3g, peptone 10g, sodium chloride 5g, 1L water).

[0028] Purification and Identification:

[0029] 12 liters of Bacillus megaterium were cultured (160 rpm, 37°C, 3 days), and the fermentation broth was centrifuged at 8,000 r / min for 20 minutes. The centrifuged supernatant was precipitated with 80% ammonium sulfate, and after the precipitate was freeze-dried, it was chromatographed on Sephadex G-25 with a flow r...

Embodiment 2

[0042] Antibacterial test on solid medium

[0043] The 24 kinds of short peptides (all L-type) obtained above were subjected to the antibacterial effect test on Aspergillus flavus, respectively named as: a, b, c, d, e, f, g, h, i, j, k ,l,m,n,o,p,q,r,s,t,u,v,w,x, and compared with laboratory disinfectant-75% alcohol and strong fungicide-1% potassium permanganate Carry out the evaluation of antibacterial effect (repeat test 3 times). Among them, the specific test methods are:

[0044] Prepare solid PDA medium, pour the PDA medium into a 96-well plate to solidify; spread 4 microliters on the PDA medium for 10 6 Aspergillus flavus spores / ml, then take 10 microliters of various short peptides of 16 mg / ml, 75% alcohol, and 1% potassium permanganate and apply them to each sample well on a 96-well plate, and cultivate them at 28°C for 3 days. Days to observe the antibacterial results.

[0045] like Figure 4As shown, the obtained 24 kinds of short peptides have a certain inhibit...

Embodiment 3

[0047] Bacteriostasis test in crop storage

[0048] The crops in this embodiment can be peanuts, corn, rice, cottonseed, nuts, etc., wherein, taking peanut storage as an example, the details are as follows:

[0049] First of all, it should be noted that since the 24 short peptides have been proved to have good antibacterial effects through Example 2, this example only uses Asp-Orn, Asp-Asn and Asp-Asn as examples for testing, and at the same time , in order to judge the impact of the configuration of each peptide on the antibacterial effect, the synthesis of different configurations was carried out by taking the above three peptides as examples. The resulting short peptides of different configurations were made into solutions of different concentrations, sprayed onto peanuts, and cultivated at 28°C for 7 days to observe the growth of Aspergillus flavus (as a control comparison: each peanut was inoculated with 10 5 aflatoxin spores).

[0050] Depend on Figure 5 It can be se...

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Abstract

The invention provides an ornithine contained short chain polypeptide and an application thereof, and belongs to the field of a biotechnology. The short chain polypeptide can be effectively applied torestraining growth of aspergillus flavus and biosynthesis of aflatoxin. The short chain polypeptide has the structure as shown in the following general formula, wherein X represents ornithine, n is equal to 1, and m is a positive integer between 1-8. The ornithine contained short chain polypeptide disclosed by the invention can be applied to restraining the growth of the aspergillus flavus.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a short peptide containing ornithine and its application. Background technique [0002] Aflatoxins are mainly produced by Aspergillus flavus and Aspergillus parasiticus, which are highly toxic, teratogenic and carcinogenic. Crops such as peanuts, corn, rice, cottonseed, and nuts are easily contaminated by aflatoxins. Aflatoxin B1 is the most toxic natural carcinogen known, which mainly causes liver cancer. [0003] Physical, chemical and biological methods are commonly used to remove aflatoxin from raw materials. Physical methods such as heating, adsorption, extraction, gamma ray irradiation, ultraviolet irradiation, etc. Chemical methods such as treatment with ozone, ammonia, chlorine, hydrogen peroxide, and formaldehyde. Biological methods, such as detoxification of raw materials by microbial fermentation, and inhibition of toxin production by plant extracts. However, each meth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/32A23L3/3526A61K38/05A61K38/06A61K38/07A61K38/08A61P31/04
CPCC07K14/32A23L3/3526A61P31/04A23V2002/00A61K38/00A23V2250/55
Inventor 孔青
Owner OCEAN UNIV OF CHINA
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