Preparation method and applications of human blood apolipoprotein A1 product

Abstract

The present invention discloses a preparation method and applications of a human blood apolipoprotein A1 product, and belongs to the field of biopharmaceuticals. The product not only can reduce bloodlipids, more importantly also can remove atherosclerotic plaques, can be used for prevention and treatment of arteriosclerotic cardio-cerebral vascular diseases, is a world-class major innovative drug, can open a new era of the cardio-cerebral vascular diseases in human body treatment based on atherosclerosis as pathological basis. The preparation method of the human blood apolipoprotein A1 product is as follows: using an ammonium sulfate solution at an appropriate concentration to separate apolipoprotein A1 from human plasma, removing dextran sulfate from the obtained apolipoprotein A1 component by using potassium oxalate, conducting ultrafiltration to remove impurities, and then conducting sterilization and filtration, virus inactivating, sub-packaging, freeze-drying, etc. to obtain thehuman blood apolipoprotein A1 product. The preparation method comprehensively utilizes various protein components in plasma, is simple in operation and relatively low in investment. The obtained product is good in stability, safety, etc.

Description

technical field

[0001] The invention relates to a human blood apolipoprotein A1 product, in particular to a preparation method and application of a human blood apolipoprotein A1 product, and belongs to the field of biopharmaceuticals. Background technique

[0002] Cardiovascular and cerebrovascular diseases based on atherosclerosis (AS) are major diseases that endanger human health, accounting for more than 40% of human mortality. People will undoubtedly attach great importance to this type of disease, and the medical community has invested a lot of manpower and material resources in researching its prevention and treatment methods.

[0003] In the middle and late part of the last century, many types (multiple) of lipid-lowering drugs (statins, etc.) came out successively, which played a certain role in alleviating the harm of such diseases. However, lipid-lowering drugs can only slow down the development of atherosclerosis (AS), but no drug has been developed to remove the...

Examples

preparation example Construction

[0018] The present invention provides a kind of preparation method of human blood apolipoprotein A1 product, it comprises the following steps:

[0019] Step 1: Take an appropriate amount of plasma and dilute it with distilled water, add sodium citrate to 0.1%-1%, add sodium chloride to 0.2%-1.5%, stir well, add saturated ammonium sulfate solution to 30%-80% saturation After stirring again, place it to make it settle completely, filter it with a filter cloth or centrifuge it with a continuous flow centrifuge, and transfer the precipitate for other purposes; collect the filtrate or centrifuged supernatant, and add ammonium sulfate to a concentration of 50%-95% , after the precipitation is complete, use filter cloth to filter or continue to centrifuge, transfer the supernatant to other uses, and collect the secondary precipitation for later use;

[0020] Step 2, take the secondary precipitate in step 1 and add an appropriate amount of water to dissolve, remove residual ammonium s...

technical field

[0001] The invention relates to a human blood apolipoprotein A1 product, in particular to a preparation method and application of a human blood apolipoprotein A1 product, and belongs to the field of biopharmaceuticals. Background technique

[0002] Cardiovascular and cerebrovascular diseases based on atherosclerosis (AS) are major diseases that endanger human health, accounting for more than 40% of human mortality. People will undoubtedly attach great importance to this type of disease, and the medical community has invested a lot of manpower and material resources in researching its prevention and treatment methods.

[0003] In the middle and late part of the last century, many types (multiple) of lipid-lowering drugs (statins, etc.) came out successively, which played a certain role in alleviating the harm of such diseases. However, lipid-lowering drugs can only slow down the development of atherosclerosis (AS), but no drug has been developed to remove the...

Examples

preparation example Construction

[0018] The present invention provides a kind of preparation method of human blood apolipoprotein A1 product, it comprises the following steps:

[0019] Step 1: Take an appropriate amount of plasma and dilute it with distilled water, add sodium citrate to 0.1%-1%, add sodium chloride to 0.2%-1.5%, stir well, add saturated ammonium sulfate solution to 30%-80% saturation After stirring again, place it to make it settle completely, filter it with a filter cloth or centrifuge it with a continuous flow centrifuge, and transfer the precipitate for other purposes; collect the filtrate or centrifuged supernatant, and add ammonium sulfate to a concentration of 50%-95% , after the precipitation is complete, use filter cloth to filter or continue to centrifuge, transfer the supernatant to other uses, and collect the secondary precipitation for later use;

[0020] Step 2, take the secondary precipitate in step 1 and add an appropriate amount of water to dissolve, remove residual ammonium s...

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