Hansenula anomala SY1805 and application thereof to preparation of mulberry borer attractant

A technology of Hansenula anomaly and SY1805, which is applied in the fields of attractants, applications, chemicals for biological control, etc., can solve the problems of complex extraction and preparation process, weak specificity, single components, etc., and achieves application prospects Good, attractive and simple preparation process

Active Publication Date: 2019-12-27
北京恩格兰环境技术有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these attractants are conventional materials, the specificity is not strong, and the extraction and preparation process is relatively complicated
"Preliminary report on the control of mulberry borer with biological food at

Method used

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  • Hansenula anomala SY1805 and application thereof to preparation of mulberry borer attractant
  • Hansenula anomala SY1805 and application thereof to preparation of mulberry borer attractant
  • Hansenula anomala SY1805 and application thereof to preparation of mulberry borer attractant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Obtaining of Hansenula anomalies SY1805:

[0030] Screening of yeast strains:

[0031] 1. Preparation of culture medium:

[0032] YPD medium: peptone 20g; yeast extract powder 10g; glucose 20g; distilled water 1000mL; pH value 6.5.

[0033] PDA medium: potato 200g; glucose 20g; distilled water 1000ml; natural pH.

[0034] For solid medium, 1.5% agar was added on the basis of liquid medium, and all medium was sterilized at 115°C for 20 minutes.

[0035] 2. Preliminary screening: Take rhizosphere soil and decayed leaf samples in a mulberry garden in Ningnan County, Liangshan Prefecture, Sichuan Province, take 10g samples and add them to a triangular flask containing 100mL sterile saline, shake at 200r / min for 30min at 30°C The bacterial suspension was serially diluted with sterile normal saline, and an appropriate dilution was selected, spread on YPD plates (adding ampicillin), cultured at 30°C for 2-3 days, and observed the colony morphology. The colonies of suspecte...

Embodiment 2

[0047] Component analysis of wort and mulberry juice fermented by Hansenula anomalies SY1805:

[0048] The method described in Example 1 is used to make wort with a sugar content of 15°Brix, then add fresh mulberry juice of 20% (V / V) of the wort volume (directly squeezed and filtered by a juice extractor, without adding water), and inoculate the malt 5% of the juice volume of Hansenula anomalies SY1805 fermentation liquid (YPD liquid medium fermentation culture, OD 600 5.0), and then fermented for 72 hours at 30°C and 100r / min to obtain a fermentation broth.

[0049] The other group did not add mulberry juice, and directly fermented with wort, and the two groups of liquids did not need to be sterilized. The Y-type olfactometer described in Example 1 was used to test the lure of the two fermented liquids to the mulberry borer.

[0050] The results show that the fermented liquid containing mulberry juice has an attractive rate of 75% to the mulberry borer, which is higher tha...

Embodiment 3

[0052] Analysis of volatile components in mixed fermentation broth of Hansenula anomalies SY1805 wort and mulberry juice:

[0053] The odors of the two fermentation broths in Example 2 were identified by gas chromatography-mass spectrometry (GC-MS). Gas chromatography is Agilent 6890A, mass spectrometry is 5975C, DB-5 column (30m×0.25mm×0.25μm). The heating program is as follows: the furnace temperature is maintained at 40°C for 1 min, then raised to 120°C at 5°C / min and maintained for 2 minutes; then raised to 250°C at 10°C / min and maintained for 2 minutes. The mass spectrometer transmission line temperature is 250°C, the ion source temperature is 230°C, and the ion current is 70e V; the scanning range is 50-300m / z.

[0054] Table 1 shows that a total of 50 volatile compounds were detected in the volatile components of the two fermentation broths, including 21 esters, 4 alcohols, 4 acids, 2 ketones, 7 aldehydes, and 2 phenols , 10 other categories.

[0055] A total of 36 a...

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Abstract

The invention belongs to the field of fruit tree pest attractants, particularly relates to hansenula anomala SY1805 and application thereof to preparation of a mulberry borer attractant, and particularly discloses the Hansenula anomala SY1805, and the collection number is CCTCC NO: M2019719. Wort fermentation liquid of the strain has attractive effect on the mulberry borer, and the mixed fermentation liquid added with mulberry juice has better attractive effect. The invention further provides the mulberry borer attractant. The mulberry borer attractant is prepared bymixing ethyl alcohol, acetic acid, white granulated sugar, fructose syrup, anhydrous calcium sulfate and hansenula anomala fermentation liquid, and can be used for green prevention and treatment of mulberry borer in the mulberry field.

Description

technical field [0001] The invention belongs to the field of attractants for fruit tree pests, and in particular relates to Hansenula anomalies SY1805 and its application in the preparation of attractants for mulberry borers. Background technique [0002] Mulberry is an important perennial economic crop in my country, with a wide range of cultivation, from the south of Harbin in the northeast; to Xinjiang and Qinghai in the northwest; to Guangdong and Guangxi in the south, to Taiwan in the east; to Sichuan and Yunnan in the west; . Mulberry has a wide range of uses. Mulberry, mulberry branches, mulberry leaves, and mulberry bark all contain special medicinal ingredients and have good medicinal effects. Mulberry leaves are also the only source of feed for silkworms. The quality and output of mulberry leaves are the decisive factors affecting the development of sericulture, and they are also the main restrictive factors for the development of my country's silk weaving industry...

Claims

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Application Information

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IPC IPC(8): C12N1/16A01N63/04A01P19/00C12R1/645
CPCC12N1/145C12R2001/645
Inventor 贺福平吴建华
Owner 北京恩格兰环境技术有限责任公司
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