A method for producing tridecanedioic acid by fermentation of Candida vissi

Abstract

This product relates to a method for the production of long-chain tridecanedioic acid by using Candida viesis biological method, including the following steps: seed culture stage, fermentation culture stage and tridecanedioic acid separation and recovery stage, said The deposit number CCTCC M No. 2019074 of Candida viesis. Wherein, the strain growth OD value of the seed liquid obtained in the seed culture stage reaches 0.6-0.85. The tridecanedibasic acid produced by the method of the present invention has a satisfactory crystal form, and is more likely to meet the export requirements of dust-free and low-dust products in Europe; and is particularly suitable for processing and utilization as raw materials for high-grade lubricating oil and high-end car spraying materials .

Description

A kind of method for Candida viscera fermentation to produce tridecanediacid technical field The invention belongs to the field of biochemical industry, relate to a kind of method for Candida viscera fermentation to produce tridecanedicarboxylic acid and products and strains. Background technique [0002] Long-chain diacids refer to diacids with ten or more carbon atoms, generally straight-chain diacids. muskone It is one of the main effective components with physiological activity in natural musk, and can be formulated into a variety of precious Chinese musk instead of natural musk. It has the curative effect of antibacterial and anti-inflammatory, clearing the meridian and promoting blood circulation. Musk‑M made from mixed dibasic acids and glycol esters for the production of high Grade spice provides new raw materials and important raw materials for high-grade nylon hot-melt adhesives and high-grade coatings for clothing. Tridecanediacid (DC13) is musk-T synth...

AI Technical Summary

Problems solved by technology

However, in the existing method of biologically synthesizing tridecanedibasic acid, there is still a low level of fermentation, and the purity of the product cannot meet the requirements for the preparation of long-chain dibasic acid esters, especially in dibasic acid products due to fermentation strain α , the difference of the ω-oxidation site will produce a certain amount of monobasic acid impurities. In addition, the result of the fermentation method of the prior art is: after 70 hours, a certain amount of nC13 is added every day, so that the normal alkane concentration in the fermentation broth is always > 5% (v / v)

After 139 hours of fermentation, the amount of acid produced reached 235.8g / L. After 163 hours of fermentation, the amount of acid produced reached 265g / L. However, the operation steps of the above method are relatively complicated, and the entire fermentation cycle is not shortened, and the production equipment takes a long time. Can not solve the problem of dibasic acid industrialization