Method for producing tetradecanedioic acid by fermentation of candida viswanathii
A technology of tetradecanedibasic acid and Candida viesis, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problem of long production equipment time and product purity not reaching long-chain binary Problems such as acid esters and low fermentation level, etc., to achieve the effect of dense crystal form
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Embodiment 1
[0030] The process of fermentative synthesis of long-chain dibasic acids is as follows:
[0031] 1. Culture medium preparation
[0032] ① Incline medium: malt juice agar medium;
[0033] ②Seed medium: glucose 25g / L, disodium hydrogen phosphate 6g / L, yeast extract 3g / L, corn steep liquor 5g / L, urea 1.5g / L, NaCl 0.5g / L, KCl 1g / L, Tween 0.5 g / L.
[0034] ③ Fermentation medium: glucose 40g / L, disodium hydrogen phosphate 8g / L, yeast extract 3g / L, corn steep liquor 5g / L, urea 1.5g / L, NaCl 0.5g / L, KCl 1g / L, Tween 0.5 g / L.
[0035] 2. Seed cultivation stage
[0036] A piece of Candida viswanathii ws-1401 was spread on a solid slant medium in a large test tube of 20×180 mm, and cultured at 27°C for 72 hours. Inoculate the activated seed culture on the slant into a 500ml Erlenmeyer flask containing 200ml of seed medium, and culture it with shaking at 27°C and 250r / min for 48 hours, and the detected strain OD is between 0.6 and 0.85.
[0037] 3. Fermentation acid production stage: ...
Embodiment 2
[0041] The process of fermentative synthesis of long-chain dibasic acids is as follows:
[0042] 1. Culture medium preparation
[0043] ① Incline medium: malt juice agar medium;
[0044] ②Seed medium: glucose 25g / L, disodium hydrogen phosphate 6g / L, yeast extract 3g / L, corn steep liquor 5g / L, urea 1.5g / L, NaCl 0.5g / L, KCl 1g / L, Tween 0.5 g / L.
[0045] ③ Fermentation medium: glucose 35g / L, disodium hydrogen phosphate 8g / L, yeast extract 3g / L, corn steep liquor 5g / L, urea 1.5g / L, NaCl 0.5g / L, KCl 1g / L, Tween 1.5 g / L.
[0046] 2. Seed cultivation stage
[0047] A piece of Candida viswanathii ws-1401 was spread on a solid slant medium in a large test tube of 20×180 mm, and cultured at 27°C for 72 hours. Inoculate the activated seed culture on the slant into a 500ml Erlenmeyer flask containing 200ml of seed medium, and culture it with shaking at 27°C and 250r / min for 48 hours. In a base 10L fermenter, the initial pH is 6.0, the temperature is 27°C, the ventilation rate is 1:0...
Embodiment 3
[0052] The process of fermentative synthesis of long-chain dibasic acids is as follows:
[0053] 1. Culture medium preparation
[0054] ① Incline medium: malt juice agar medium;
[0055] ②Seed medium: glucose 25g / L, disodium hydrogen phosphate 6g / L, yeast extract 3g / L, corn steep liquor 5g / L, urea 1.5g / L, NaCl 0.5g / L, KCl 1g / L, Tween 0.5 g / L.
[0056] ③ Fermentation medium: glucose 35g / L, disodium hydrogen phosphate 8g / L, yeast extract 3g / L, corn steep liquor 5g / L, urea 1.5g / L, NaCl 0.5g / L, KCl 1g / L, Tween 1.5 g / L.
[0057] 2. Seed cultivation stage
[0058] A piece of Candida viswanathii ws-1401 was spread on a solid slant medium in a large test tube of 20×180 mm, and cultured at 27°C for 72 hours. Inoculate the activated seed culture on the slant into a 500ml Erlenmeyer flask containing 200ml of seed medium, and culture it with shaking at 27°C and 250r / min for 48 hours. In the base 5L fermenter, the initial pH is 6.0, the temperature is 27°C, the ventilation rate is 1:...
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