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Novel biological fermentation method for normal long-chain tridecanedioic acid

A thirteen carbon dibasic acid and biological method technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problem that the product purity cannot reach the long-chain dibasic acid ester, the fermentation level is not high, Inconvenience and other issues

Active Publication Date: 2017-11-03
青岛智库生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the existing method of biologically synthesizing tridecanedibasic acid, there is still a low level of fermentation, and the purity of the product cannot meet the requirements for the preparation of long-chain dibasic acid esters, especially in dibasic acid products due to fermentation strain α , Different ω-oxidation sites will produce a certain amount of monobasic acid impurities. In addition, the products obtained by fermentation methods in the prior art are usually powdery products, which brings great inconvenience to the subsequent synthesis of high-grade spices or synthetic nylon.

Method used

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  • Novel biological fermentation method for normal long-chain tridecanedioic acid
  • Novel biological fermentation method for normal long-chain tridecanedioic acid
  • Novel biological fermentation method for normal long-chain tridecanedioic acid

Examples

Experimental program
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Effect test

Embodiment 1

[0061] (1) Take an inoculation loop of Candida tropicalis C1301 cells, spread it on the wort solid slope of a 15×180 large test tube, and cultivate it at 28° C. for 2 days.

[0062] (2), get one of the above strains, insert it into a 250ml Erlenmeyer flask with 30ml liquid seed culture medium and cultivate it for 40 hours at 28-30°C on a rotary shaker at 220 rpm. Liquid seed medium contains KH 2 PO4 8g / l, yeast extract 5g / l, corn steep liquor 3g / l, sucrose 30g / l, urea 3g / l, tap water, pH 5.0.

[0063] (3) In a 500ml Erlenmeyer flask containing 15ml of fermentation medium, insert 3.5ml of the above-mentioned cultivated seed solution, ferment for 4 days at 28-30°C, 220 rpm on a rotary shaker, and use 6N every 24 hours Adjust the pH to 7.5-8.0 once with NaOH. The fermentation medium mixture contains KH 2 PO 4 10g / l, sucrose 20 / l, yeast extract 3g / l, corn steep liquor 3.5g / l, urea 1.5g / l, and nC13200ml / l, tap water configuration, PH7.2. Sterilize at 110°C for 30 minutes.

[...

Embodiment 2

[0066] (1) Seed medium and culture method and fermentation medium are the same as example 1.

[0067] (2) Cultivate 3000mL for two days, OD(×30, 620nm) is 0.81pH 3.8, strong, no miscellaneous bacteria seed solution into the first-level seed tank with 700L seed medium, sterilized at 121°C for 40 minutes , 29°C 350 rpm, tank pressure 0.8kg / cm 2 , ventilation rate 1:0.8, cultured for 36 hours, as the seeds of the secondary seed mother.

[0068] (3) Insert the 700L seed solution cultivated in (2) into a 6.5m 3 Seed medium, 10m after sterilized at 121°C for 40 minutes 3 In the secondary seed tank, 30°C, 200 rpm, tank pressure 1kg / cm 2 , ventilation rate 1:0.7, cultivated for 40 to 48 hours, as fermented seeds.

[0069] (4) Connect the strong and bacteria-free seed solution cultivated in (3) into a 33m 3 Fermentation medium, 50m of sterilized at 121°C for 40 minutes 3 In a fermenter, 30°C, 200 rpm, tank pressure 1kg / cm 2 , ventilation volume 1:0.5, at the beginning, nC 13 4...

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Abstract

The invention relates to a novel method for producing normal long-chain tridecanedioic acid from candida tropicalis by biological fermentation, which includes: a starter culture stage of candida tropicalis C1301, a fermentation culture stage of candida tropicalis C1301 and a separation and recovery stage of tridecanedioic acid as a product in fermented liquor. The normal long-chain tridecanedioic acid produced by the method disclosed by the invention has a satisfactory lamellar morphology, and can more easily meet the requirement of Europe on exported dustless, low-dust products; and moreover, the normal long-chain tridecanedioic acid is particularly suitable for being utilized in the material processing of high-grade lubricating oil and high-grade nylon resin.

Description

technical field [0001] The invention belongs to the field of biochemical industry, and relates to a method for biologically synthesizing and producing long-chain tridecane dibasic acid, a bacterial strain and a product of positive long-chain tridecane dibasic acid. Background technique [0002] Long-chain dibasic acids refer to dibasic acids with ten or more carbon atoms, and generally refer to straight-chain dibasic acids. And muscone is one of the main effective ingredients with physiological activity in natural musk, which can replace natural musk to be formulated into various famous and precious Chinese patent medicines, which have the effects of antibacterial, anti-inflammation, stimulating menstruation and activating blood circulation. Musk-M made of mixed dibasic acid and ethylene glycol ester provides new raw materials for the production of high-grade fragrances and important raw materials for high-grade nylon hot melt adhesives and high-grade coatings for clothing. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12N1/16C07C55/02C12R1/74
CPCC07C55/02C12P7/6409C12N1/165C12R2001/74
Inventor 史兰东修德恒李刚
Owner 青岛智库生物技术有限公司