A kind of soybean Phytophthora inhibitor and preparation method thereof
A technology of Phytophthora sojae and an inhibitor, which is applied in the field of Phytophthora sojae inhibitor and its preparation, can solve the problems of killing beneficial microorganisms, human and animal poisoning, plant phytotoxicity, etc., and achieves improved resistance to infection and safety of Phytophthora sojae High, no pesticide residue effect
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Embodiment 1
[0028] A soybean pyrofici inhibitor, comprising no mercyl propyl esters and sebacol, and sebbium as a co-acid propylene solvent, the amount ratio ratio of 0.1 mg-0.3 mg: 1-2 mmol No mercyl ester.
[0029] Such as Figure 1-7 As shown, the mechanism of ketchloride inhibits soyphydrate by proteomics: Soypopide in the untreated soybean Phytophthoraic and Nothic Acid (add no food in liquid V8 to make the final concentration 0.5 mmol / L) was incorporated in 3-5 days of collecting mycelium, and the liquid is mixed into the powder. Each group sample added a powder 4x volume phenol extraction buffer (containing 1% protease inhibitor), ultrasonic cleavage. The equivalent Tris balance phenol, 4 ° C, 5500 g centrifuge for 10 min, and the upper clearance was obtained and added to a magnification of 0.1 M acetate / methanol precipitation overnight, and the protein precipitate was washed with methanol and acetone. Solution. Dithionic sugar was added to the protein solution to give a final conc...
Embodiment 2
[0043] The yoglycol propyl ester was prepared by the catalysis of no food acid and propanol in a tannin enzyme, including the following preparation steps:
[0044] (1) No food acid extraction process: adopts supercritical carbon dioxide extraction methods, weigh the particle size of 2-5 mm size blueberry leaf or green tea powder 1.3-1.8kg, add 17-22% temperature of 2-8 ° C In the pure water, it was placed at 5 ° C for 50-70 minutes, and the supercritical carbon dioxide extraction pressure was 23-27 MPa, and the extraction time was 32-50 minutes, and there was no food acid;
[0045] (2) Tannin enzyme preparation process: Aspergillus mildew in liquid potato medium for 3-5 days, collecting fermentation broth, precipitation with ammonium sulfate precipitation, reconstall with crude protein, ultrafiltration with intercept molecular weight 10 kDa The ammonium sulfate was filtered off and concentrated the protein to give a tanning enzyme;
[0046] (3) Non-serial acid propyl ester synthes...
Embodiment 3
[0057] The choice of solvent and the formulation of the solution: the solution is indicator, from ethanol, propanol, butanol, pentanol, hexanol, acetanol, dimethyl sulfoxide, ethyl acetate, petroleum ether, and sepa glycol, ether, etc. Among the organic solvents, it is preferred to use sebicyol as a co-acid propylene reagent, which is used in addition to: 0.1 mg-0.3 mg of sepa glycol: 1-2 mmol, no mercyl ester, completely dissolved, 60-70 The hot water and room temperature of ° C are 500 ml of water, which can be used for the prevention and treatment of soybean Phytophthora. As shown in table 2
[0058]
[0059] Table 2. Solvent type and amount optimization experiment
[0060] No mercageptate can also stimulate the defense reaction of plants, and improve resistance gene expression to resist infection of soybean. Follow the TAKARA reagent and extracting mRNA anti-transcription cDNA and carried out in accordance with the QRT-PCR system: 2 μl of C DNA template, 12.5 μL of Sybr Gree...
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