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Anti-cd97 monoclonal antibody, its variable region and constant region sequence and application

A monoclonal antibody, variable technology, applied in the direction of antibodies, antibody mimics/scaffolds, anti-receptors/cell surface antigens/cell surface determinants immunoglobulins, etc., can solve the problem of lack of anti-CD97 monoclonal antibodies, etc. Achieve the effect of inhibiting proliferation, improving patient survival, and reducing invasiveness

Active Publication Date: 2021-09-21
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no anti-CD97 monoclonal antibody with therapeutic properties

Method used

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  • Anti-cd97 monoclonal antibody, its variable region and constant region sequence and application
  • Anti-cd97 monoclonal antibody, its variable region and constant region sequence and application
  • Anti-cd97 monoclonal antibody, its variable region and constant region sequence and application

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Embodiment 1

[0033] The binding constant between CD97-His (11280-H08H) and CD97 antibody (Mouse Mab) was measured by ProteOn XPR36 biosensor, the chip was GLC (Bio-Rad, Hercules, CA), and the temperature was 25°C. CD97 (170RU) was coupled to the ligand channel using a coupling agent (400mM EDC and 100mM SNHS mixed solution 1:1, diluted 1 / 500) for 3 minutes, the concentration was 15μg / mL, and the buffer solution was 10mM acetic acid Sodium solution (pH=4.5), and finally the remaining activated sites were blocked with 1M ethanolamine hydrochloric acid solution. The surface of the chip was washed with IgG dilution buffer solution and reprocessed. The experimental mobile phase used PBST (pH=7.4).

[0034] The purified CD97 mouse antibody was injected within 3 minutes in the "one-shotkinetic" mode, and tested at four concentrations of 0.8333nM, 1.67nM, 3.33nM, and 6.67nM. CD97 antibody and CD97 protein binding test results are as follows figure 2 and Table 1, where, Figure 2-1 For the ant...

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Abstract

The invention relates to an anti-CD97 monoclonal antibody, its variable region and constant region sequences and applications, and belongs to the field of biotechnology. The variable region sequence of the anti-CD97 monoclonal antibody of the present invention includes the heavy chain sequence shown in SEQ ID NO:1 and the light chain sequence shown in SEQ ID NO:2. The anti-CD97 monoclonal antibody prepared from the variable region sequence of the present invention can specifically bind to CD97, and can be used for the preparation of therapeutic drugs for diseases (such as brain tumors) related to excessive and out-of-control expression of CD97.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an anti-CD97 monoclonal antibody with biological activity of specifically binding to CD97, its variable region and constant region sequences and application against brain tumors. Background technique [0002] Malignant glioma is the most common type of brain tumor clinically, and its incidence rate is as high as 60% in brain tumors. Glioblastoma multiforme (GBM) is the most malignant type of malignant glioma. The incidence of malignant glioma in my country is 1-4 / 100. Glioma stem cells (GSC) have the characteristics of general stem cells, that is, self-renewal ability and differentiation, which play a decisive role in the invasion, drug resistance, and radiation resistance of glioma. [0003] CD97 is a dimeric glycoprotein with seven transmembrane structures, its molecular weight is between 75 and 85 kDa, and it is mainly continuously expressed on the surface of macrophages / monocyt...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/28C07K19/00C12N5/10A61K39/395A61P35/00
CPCA61K2039/505A61P35/00C07K14/7051C07K16/2896C07K2317/52C07K2317/56C07K2319/00
Inventor 尹金龙师冰洋吴海刚
Owner HENAN UNIVERSITY