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MiR-182 transcription inhibition factor and application of miR-182 transcription inhibition factor to osteosarcoma

A technology of transcriptional repressor, mir-182, applied in the medical field

Inactive Publication Date: 2016-04-27
胡军 +3
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, whether the regulation of TIAM1 by miR-182 also exists in other types of cancer remains to be further studied

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  • MiR-182 transcription inhibition factor and application of miR-182 transcription inhibition factor to osteosarcoma
  • MiR-182 transcription inhibition factor and application of miR-182 transcription inhibition factor to osteosarcoma
  • MiR-182 transcription inhibition factor and application of miR-182 transcription inhibition factor to osteosarcoma

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Embodiment Construction

[0065] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0066] In the present invention, a new target gene of miR-182 was discovered by means of different experimental means: a potential miR-182 recognition site was found in the 3-terminal non-coding region (UTR) of the TIAM1 gene predicted by Targetscan software, luciferase experiment It was confirmed that miR-182 can effectively bind to the recognition site, thereby inhibiting the expression of TIAM1; overexpression of miR-182 in the osteosarcoma cell line MG-63 also inhibited the expression of TIAM1 at the mRNA level and protein level; miR-182 The reduction of expression level will lead to abnormal expression of TIAM1 and abn...

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Abstract

The invention discloses a miR-182 transcription inhibition factor and an application of a miR-182 transcription inhibition factor to osteosarcoma. The miR-182 transcription inhibition factor has a miR-182 transcription inhibition factor sequence SEQ ID NO. The application includes an application to an osteosarcoma cell system, an application to tissues of an osteosarcoma patient, an application to cell proliferation marker genes Ki-67 and PCNA, and an application to a TIAM1 gene in an osteosarcoma cell. According to the application, the expression of the miR-182 in the osteosarcoma cell system and the tissues is remarkably reduced, the over-expression of the miR-182 remarkably inhibits the tumor growth, the level of the miR-182 in regulating and controlling the expression of the cell proliferation markers Ki-67 and PCNA is remarkably improved, the miR-182 in the osteosarcoma cell directly targets the TIAM1 gene, and the miR-182 influences the occurrence, development and deterioration of the osteosarcoma by regulating and controlling the expression of the TIAM1.

Description

technical field [0001] The invention belongs to the field of medical technology, and in particular relates to a miR-182 transcription inhibitor and its application in osteosarcoma. Background technique [0002] The English name of tumor is Tumor, and the English name of cancer is Cancer. These two terms are often used interchangeably. However, in a strict sense, the meaning of tumor and cancer is not the same. In fact, a tumor is not necessarily cancer. In the sense of the word, a tumor refers to a mass of cells. In English, tumor (Tumor) is closer to neoplasm (Neoplasm), which means a group of abnormally proliferating cells. Such cells proliferate significantly faster than ordinary normal cells. If left untreated, it often continues to proliferate, causing abnormalities in the body. According to the degree of harmfulness of the proliferating cells, tumors / neoplastic growths can be divided into two categories: benign tumors and malignant tumors. As the name implies, b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N15/70C12Q1/68
CPCC12N15/1135C12N2310/141C12Q1/6886
Inventor 胡军刘志明周曙光周玉成吕国华秦榕胡正黄晓云胡潇予
Owner 胡军
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