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Preparation method of electrochemical immunosensor for detecting prostate specific antigen

A prostate-specific, immunosensor technology, applied in the fields of immunoassay, biosensing, and functional nanocomposite materials, can solve the problems of high cost, poor stability, and low sensitivity of prostate-specific antigen detection, and achieve excellent enrichment, The effect of low cost and improved sensitivity

Inactive Publication Date: 2020-01-14
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at the current problems of high cost, low sensitivity, and poor stability of prostate-specific antigen detection, the present invention proposes a method for preparing an electrochemical immunosensor for detecting prostate-specific antigen. The specific steps are as follows:

Method used

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  • Preparation method of electrochemical immunosensor for detecting prostate specific antigen
  • Preparation method of electrochemical immunosensor for detecting prostate specific antigen
  • Preparation method of electrochemical immunosensor for detecting prostate specific antigen

Examples

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Embodiment 1

[0046] A method for preparing an electrochemical immunosensor for detecting prostate specific antigen, the specific steps are as follows:

[0047] (1) Preparation of AuNPs: Under stirring, 1.0 mL of 23.46 mmol / L HAuCl 4 Add the solution to 80 mL of boiling deionized water, stir at 80°C for 15 min, quickly add 8 mL of sodium citrate solution with a concentration of 14.55 mmol / L, and reflux for 20 min until the solution turns wine red, and the solution is naturally cooled to room temperature to obtain AuNPs dispersed solution and stored at 4°C;

[0048] (2) Preparation of black phosphorene (BPene): Weigh 10 mg of black phosphorous crystals, grind them into powder, and disperse them in 20 mL of deionized water. Ultrasonicate for 5 hours in an ice bath in the dark, with ultrasonic power of 300 W, and centrifuge at 3000 rpm for 5 minutes to remove residual untreated phosphorus. For the shedding massive black phosphorus particles, the collected supernatant was centrifuged at 9000rp...

Embodiment 2

[0066] A method for preparing an electrochemical immunosensor for detecting prostate specific antigen, the specific steps are as follows:

[0067] (1) Preparation of Au NPs: under stirring, 1.2 mL of 23 mmol / L HAuCl 4 Add the solution into 100mL boiling deionized water, stir at 100°C for 10min, then quickly add 10mL of sodium citrate solution with a concentration of 16mmol / L, and reflux for 30min until the solution turns wine red, and the solution is naturally cooled to room temperature to obtain the AuNPs dispersion , and stored at 4°C;

[0068] (2) Preparation of black phosphorene (BPene): Weigh 15 mg of black phosphorous crystals, grind them into powder and disperse them in 30 mL of deionized water, and ultrasonicate for 6 h in an ice bath in the dark, with the ultrasonic power at 400 W, and centrifuge at 3000 rpm for 10 min to remove Unshed massive black phosphorus particles remained, and the collected supernatant was centrifuged at 9000 rpm for 10 minutes, and freeze-dri...

Embodiment 3

[0086] A method for preparing an electrochemical immunosensor for detecting prostate specific antigen, the specific steps are as follows:

[0087] (1) Preparation of Au NPs: Under stirring, 1.5 mL of 25 mmol / L HAuCl 4 Add the solution to 120mL boiling deionized water, stir at 150°C for 5min, quickly add 12mL sodium citrate solution with a concentration of 14mmol / L, and reflux for 40min until the solution turns wine red, and the solution is naturally cooled to room temperature to obtain the AuNPs dispersion , and stored at 4°C;

[0088] (2) Preparation of black phosphorene (BPene): Weigh 20 mg of black phosphorous crystals, grind them into powder, and disperse them in 40 mL of deionized water, sonicate for 8 hours in an ice bath in the dark, with ultrasonic power of 500 W, and centrifuge at 3000 rpm for 20 min to remove residual untreated phosphorus. For the shedding massive black phosphorus particles, the collected supernatant was centrifuged at 9000 rpm for 20 minutes, and f...

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Abstract

The invention provides a preparation method of an electrochemical immunosensor for detecting prostate specific antigen. The method comprises the following steps of polishing a glassy carbon electrodeinto a mirror surface by using Al2O3 polishing powder, and ultrasonically cleaning; orderly dropwise adding gold nanoparticle-loaded black phosphorus nanosheet nanocomposite dispersion liquid, prostate specific antibody, ovalbumin solution, a prostate specific antigen solution and Ab2 bioprobe conjugate on the surface of the glassy carbon electrode, and flushing the electrode surface by using a phosphate buffer solution with the pH value of 7.2, thereby preparing a sandwich type electrochemical immunosensor for detecting the prostate specific antigen. The prepared electrochemical immunosensorfor detecting prostate specific antigen disclosed by the invention has the advantages of being high in sensitivity, wide in detection range, fast in detection speed, low in detection limit and convenient to operate.

Description

technical field [0001] The invention relates to the technical fields of functional nanocomposite materials, immune analysis and biosensing, in particular to a preparation method of an electrochemical immunosensor for detecting prostate specific antigen. Background technique [0002] Prostate cancer is one of the common malignant tumors of the male urinary system, which seriously affects the quality of life of patients and even threatens their lives. Early diagnosis through specific and sensitive biomarkers and analytical tools can reduce the incidence of prostate cancer and effectively improve the survival rate. Therefore, the nature and state of prostate cancer can be judged by detecting the content and amount of prostate-specific antigen. In medicine, the early diagnosis of prostate cancer has become a clinical practice by quantitatively detecting prostate-specific antigen in serum. focus of attention. The method of combining electrochemical method and immunological meth...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/30G01N27/48
CPCG01N27/308G01N27/3275G01N27/48
Inventor 李灿鹏梁还
Owner YUNNAN UNIV
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