Body lotion containing streptokinase QK and preparation method of body lotion

A body lotion, thrombolytic enzyme technology, applied in microorganism-based methods, biochemical equipment and methods, medical preparations containing active ingredients, etc., can solve the problems of rarely degrading skin superficial thrombosis and deep vein thrombosis, etc. Achieve the effect of reducing skin bruising, degrading blood clots, and reducing blood clots in the body

Inactive Publication Date: 2020-01-17
湖北真福医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, most of the body lotions on the market are mainly for cleansing the skin, and few of them can degrade superficial blood clots and deep vein thrombosis.

Method used

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  • Body lotion containing streptokinase QK and preparation method of body lotion
  • Body lotion containing streptokinase QK and preparation method of body lotion
  • Body lotion containing streptokinase QK and preparation method of body lotion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Preparation of primary seed solution

[0029] LB seed medium was prepared in a 5L Erlenmeyer flask, sterilized and cooled to room temperature, and the original strain Bacillus subtilis QK stored at -80°C was inoculated into the LB medium. The shake flask was cultured on a shaker at 37° C. and 180 rpm for 18 hours to obtain a primary seed solution.

[0030] 2. Preparation of secondary seed solution

[0031] Prepare 60L of secondary seed medium in a 200L fermenter, sterilize at 121°C for 20 minutes, start to cool down after sterilization, and inoculate the primary seed liquid when the tank temperature drops to 35-37°C. Set the temperature to "automatic" mode, 37°C; speed to 100RPM; dissolved oxygen to "correlated speed" mode, with a correlation level of 35%; air flow to 1:1VVM; tank pressure to 0.05Mpa. Cultivate under these conditions for 8 hours, stop the fermentation when the OD600 value of the fermentation broth is > 4.0, and transplant it into a fermenter.

[0...

Embodiment 2

[0045] 1. Preparation of primary seed solution

[0046] LB seed medium was prepared in a 5L Erlenmeyer flask, sterilized and cooled to room temperature, and the original strain Bacillus subtilis QK stored at -80°C was inoculated into the LB medium. The shake flask was cultured on a shaker at 37° C. and 180 rpm for 18 hours to obtain a primary seed solution.

[0047] 2. Preparation of secondary seed solution

[0048] Prepare 60L of secondary seed medium in a 200L fermenter, sterilize at 121°C for 20 minutes, start to cool down after sterilization, and inoculate the primary seed liquid when the tank temperature drops to 35-37°C. Set the temperature to "automatic" mode, 37°C; speed to 100RPM; dissolved oxygen to "correlated speed" mode, with a correlation level of 35%; air flow to 1:1VVM; tank pressure to 0.05Mpa. Cultivate under these conditions for 8 hours, stop the fermentation when the OD600 value of the fermentation broth is > 4.0, and transplant it into a fermenter.

[0...

Embodiment 3

[0061] 1. Preparation of primary seed solution

[0062] LB seed medium was prepared in a 5L Erlenmeyer flask, sterilized and cooled to room temperature, and the original strain Bacillus subtilis QK stored at -80°C was inoculated into the LB medium. The shake flask was cultured on a shaker at 37° C. and 180 rpm for 18 hours to obtain a primary seed solution.

[0063] 2. Preparation of secondary seed solution

[0064] Prepare 60L of secondary seed medium in a 200L fermenter, sterilize at 121°C for 20 minutes, start to cool down after sterilization, and inoculate the primary seed liquid when the tank temperature drops to 35-37°C. Set the temperature to "automatic" mode, 37°C; speed to 100RPM; dissolved oxygen to "correlated speed" mode, with a correlation level of 35%; air flow to 1:1VVM; tank pressure to 0.05Mpa. Cultivate under these conditions for 8 hours, stop the fermentation when the OD600 value of the fermentation broth is > 4.0, and transplant it into a fermenter.

[0...

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PUM

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Abstract

The invention relates to a body lotion containing streptokinase QK. The body lotion comprises components in parts by weight as follows: 5-10 parts of a moistening agent, 1-5 parts of a moisturizer, 1-5 parts of a softening agent, 1-6 parts of an antioxidant, 1-6 parts of an emulsifier, 2-10 parts of a lubricant, 1-5 parts of a small-molecular wetting agent, 5-10 parts of a functional component, 1-5 parts of a macromolecular moisturizer, 40-70 parts of an aqueous solution, 1-5 parts of a preservative, 1-5 parts of a moisturizer and 1-5 parts of a cell growth factor. The invention further discloses a preparation method of the body lotion. The body lotion acts on thrombus in surface capillaries or deep veins to decompose thrombus, and can reduce skin bruise marks and redness, so that the skinrecovers whiteness and moistening.

Description

technical field [0001] The invention relates to a body milk and a preparation method thereof, in particular to a body milk containing thrombolytic enzyme QK and a preparation method thereof. Background technique [0002] Thrombolytic enzyme QK is a protease that efficiently cross-links and dissolves fibrin produced by the fermentation of Bacillus subtilis. The protease can accurately target thrombolysis, has good thrombolytic effect, strong specificity, and long-lasting thrombolytic effect. [0003] In addition to red blood cells, there are many plasma proteins in blood, such as globulin and fibrinogen. When these macromolecular proteins increase, they often bond with red blood cells to form a grid, which increases the resistance of blood flow and leads to increased blood viscosity. When the viscosity in the blood increases to a certain level, thrombus will appear, and blood vessels will be blocked. There will be blood stasis and poor circulation. [0004] When the skin i...

Claims

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Application Information

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IPC IPC(8): A61K8/9789A61K8/92A61K8/73A61K8/67A61K8/34A61K8/64A61K8/66A61P7/02A61Q19/00A61Q19/02C12N9/54C12R1/125
CPCA61K8/345A61K8/64A61K8/66A61K8/678A61K8/735A61K8/922A61K8/9789A61K2800/85A61P7/02A61Q19/00A61Q19/02C12N9/54C12Y304/21062
Inventor 王业富李靖胡定邦范宇鹏胡坤坤
Owner 湖北真福医药有限公司
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