Mechanism of long non-coding RNA Gas-5 to regulate miRNA-196a and application thereof

Abstract

The invention relates to the technical field of medical diagnosis, and discloses a mechanism of long non-coding RNA gas-5 to regulate miRNA-196a and also discloses application of the mechanism of thelong non-coding RNA Gas-5 to regulate miRNA-196a in kits and chips for diagnosing and treating esophageal cancer. According to the invention, by taking esophageal squamous cell carcinoma as a model, the specific mechanism of the long-chain non-coding RNA Gas-5 to regulate miRNA-196a is developed, a long non-coding RNA Gas-5 binding site on pri-miRNA-196a and a mechanism to the binding site to interfere the generation of miRNA-196a are made clear, and multiple DGCR8 binding sites on long non-coding RNA Gas-5 and mechanisms of these binding sites to interfere generation of miRNA-196a are also made clear. The binding sites, the binding relationship and the competition relationship provided by the invention can be used for preparing kits and chips for diagnosing and treating esophageal cancer,and a new means for diagnosing and treating esophageal cancer is provided.

Description

technical field [0001] The invention relates to the technical field of medical diagnosis, in particular to the mechanism and application of long-chain non-coding RNA Gas-5 regulating miRNA-196a. Background technique [0002] Esophageal cancer is a common digestive tract tumor, and about 300,000 people worldwide die from esophageal cancer every year. Its morbidity and mortality vary widely from country to country. my country is one of the areas with a high incidence of esophageal cancer in the world, with an average of 150,000 deaths per year. There are more men than women, and the age of onset is more than 40 years old. The typical symptom of esophageal cancer is progressive dysphagia, first it is difficult to swallow dry food, then semi-liquid food, and finally water and saliva cannot be swallowed. [0003] Long non-coding RNA (long non-coding RNA, lncRNA) is a class of RNA molecules with a transcript length of more than 200 nt. They do not encode proteins, but regulate ...

AI Technical Summary

Problems solved by technology

Although the research on lncRNAs has progressed rapidly in recent years, the functions of most lncRNAs are still unclear, and the mining of new functions of lncRNAs has become one of the hotspots of current non-coding RNA research.

The long-chain non-coding RNA Gas-5 was discovered in 1998 when it was highly expressed in growth-inhibited mouse NIH3T3 fibroblasts. In humans, the long-chain non-coding RNA Gas-5 is located in the non-coding chromosome region 1q25.1, which is a Spliced, polyadenylated non-protein coding RNA, long non-coding RNA Gas-5 is abnormally expressed in a variety of tumors, but its expression and role in esophageal cancer have not been reported yet

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