Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of DGCR8 in preparing products for treating and/or preventing osteoarthritis of animals

A technology for osteoarthritis and arthritis, which is used in the preparation of products for treating and/or preventing osteoarthritis in animals

Active Publication Date: 2018-11-30
INST OF ZOOLOGY CHINESE ACAD OF SCI
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there are no reports of targeting genes related to aging or longevity to treat osteoarthritis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of DGCR8 in preparing products for treating and/or preventing osteoarthritis of animals
  • Application of DGCR8 in preparing products for treating and/or preventing osteoarthritis of animals
  • Application of DGCR8 in preparing products for treating and/or preventing osteoarthritis of animals

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Example 1. Delaying effect of DGCR8 and its C-terminal mutants on mesenchymal stem cell senescence

[0080] 1.1 With the replicative senescence of mesenchymal stem cells, the expression of DGCR8 is significantly down-regulated

[0081] Using in vitro directed differentiation technology, human mesenchymal stem cells differentiated from human embryonic stem cells can be continuously passaged from the first generation to the twelfth generation under in vitro culture conditions. The mesenchymal stem cells at the 12th passage showed obvious growth arrest, and the cell volume became larger, and the shape was flat and other cellular senescence phenotypes. The 2nd and 12th passage mesenchymal stem cells were used as the test cells, wherein the 2nd passage cells represent young cells, and the 12th passage cells represent aging cells. Extract the total protein of the two kinds of cells, use RIPA cell lysate (Beiyuntian, P0013B) to lyse the test cells, then use the BCA protein qu...

Embodiment 2

[0105] Example 2. Therapeutic effect of DGCR8 and its C-terminal mutant gene on osteoarthritis mice established by cutting the anterior cruciate ligament

[0106] Since the DGCR8-encoding lentivirus or DGCR8-mtDRBD lentivirus obtained in Example 1 has good properties of delaying the senescence of mesenchymal stem cells in vitro, this example uses the DGCR8-encoding lentivirus obtained in Example 1 or the DGCR8 C-terminal mutant Lentivirus for gene therapy in mice with anterior cruciate ligament severing-induced osteoarthritis.

[0107] 2.1 Establish an osteoarthritis mouse model induced by anterior cruciate ligament cut

[0108] The mice used were all SPF grade, male, 10 weeks old, weighing 20±2g, and were purchased from Beijing Huafukang Biotechnology Co., Ltd. Experimental equipment and materials include: isoflurane gas anesthesia apparatus, epilator, surgical scissors, scalpel, Venus scissors, suture thread, penicillin, stereoscope, 20μL micro-injector.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses application of DGCR8 in preparing products for treating and / or preventing osteoarthritis of animals. It shows that the DGCR8 has the following functions of treating and / or preventing the osteoarthritis of the animals, treating and / or preventing osteoarthritis induced by anterior cruciate ligament transaction of the animals, delaying senescence of mesenchymal stem cells, delaying replicative senescence of the mesenchymal stem cells, and delaying pathological senescence of the mesenchymal stem cells. Experiments show that the DGCR8 has the clear function of delaying senescence of the mesenchymal stem cells and has the clear treatment effect in osteoarthritis diseases, thus a new thought is provided for development of gene therapy for osteoarthritis, and the selectingrange of clinical gene therapy is expanded.

Description

technical field [0001] The invention relates to the application of DGCR8 in the preparation of products for treating and / or preventing animal osteoarthritis in the field of biomedicine. Background technique [0002] Osteoarthritis (OA) is a common degenerative disease characterized by the degeneration of articular cartilage, increased inflammation and the formation of osteophytes. Its clinical manifestations are joint pain, swelling, dysfunction, and severe cases lead to joint disability, which seriously affects the quality of life of patients. The occurrence of osteoarthritis is related to many factors, including genetics, age, gender, obesity, trauma, physical labor, endocrine, metabolism, immunity and so on. Among them, age is the most important pathogenic factor of osteoarthritis. With age, articular cartilage degenerates, the mucopolysaccharide content in the cartilage matrix decreases, the matrix loses chondroitin sulfate, the fiber component increases, and the tough...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K38/17A61K48/00A61P19/08A61P19/02A61P29/00A61P39/06
CPCA61K38/1709A61K48/005A61P19/02A61P19/08A61P29/00A61P39/06
Inventor 曲静刘光慧邓丽萍任若通刘尊鹏
Owner INST OF ZOOLOGY CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com