Compound muji granule quality control and evaluation method based on anti-hepatic fibrosis biological activity

Abstract

The present invention discloses a compound muji granule quality control and evaluation method based on anti-hepatic fibrosis biological activity, pertaining to the technical field of Chinese patent medicine quality control and evaluation. According to the method, biological potency is taken as the evaluation index, and specifically, the biological potency of anti-hepatic fibrosis is taken as the evaluation index of compound muji granules, wherein the biological potency of the anti-hepatic fibrosis refers to the biological potency of inhibiting the proliferation of hepatic stellate cells. The compound muji granule quality control and evaluation method has the advantages of simple operation, high stability, sensitivity and reliability. The method can be taken as the effective supplement of physicochemical detection of the compound muji granules. The method can control the overall quality of the anti-hepatic fibrosis and comprehensively evaluate the overall effect of the anti-hepatic fibrosis, and the method can also be used for evaluating the strength of activity among other anti-hepatic fibrosis medicines.

Description

[0001] field of invention [0002] The invention belongs to the technical field of quality control and evaluation of Chinese patent medicines, in particular to a method for quality control and evaluation of Chinese patent medicines based on the determination of anti-hepatic fibrosis biological activity, and more particularly to a method for biological quality control and evaluation of compound Muji granules . Background technique [0003] Compared with chemical drugs, Chinese patent medicines have complex ingredients, and the modernization and internationalization of their quality standards have always been the core of the development of traditional Chinese medicines. The establishment of scientific and reliable quality control and evaluation methods is the key to perfect quality standards. At present, the quality control and evaluation of Chinese patent medicines are mainly based on qualitative and quantitative analysis of chemical components. With the deepening of research ...

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Problems solved by technology

[0004] Compound Muji Granules is developed on the basis of "Muji Tang". It is composed of four traditional Chinese medicines, versicolor versicolor, dodder seed, mountain bean root, and walnut catalpa bark. protein (AFP) is elevated, widely used in clinical practice, mainly used for the treatment of hepatitis, liver cirrhosis, and liver cancer, and its curative effect is remarkable; It is contained in the "Drug Standards of the Ministry of Health" (Chinese Medicine Prescriptions Volume 16), which is included in the "Ministry of Health Drug Standards" (Chinese Medicine Prescriptions Volume 16), after being tested by alkaline copper tartrate test solution , ferric chloride test solution and mercuric potassium iodide test solution for physical and chemical experiment identification; The bark of walnut catalpa is used as a reference medicinal material for TLC identification; its biological quality control and evaluation method has not been established, therefore, it is of practical significance to establish a method for quality control and evaluation of compound Muji granules based on biological activity

[0005] At present, there are few reports on the research results of biological activity assays in the field of Chinese patent medicines; the current assay methods for the biological activity of traditional Chinese medicine anti-hepatic fibrosis drugs, or the quality evaluation or detection methods of anti-hepatic fibrosis traditional Chinese medicines based on biological activity Not yet reported

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