Antibacterial peptide bionic calcium phosphate coating and preparation method and application thereof

A biomimetic calcium phosphate, calcium phosphate technology, applied in the field of medicine, can solve the problem of lack of antibacterial properties of bone substitute materials, and achieve the effect of broad application prospects

Inactive Publication Date: 2020-01-31
XIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide an antibacterial peptide biomimetic calcium phosphate coating, which solves the problem that bone substitute materials lack antibacterial properties in the treatment process of existing oral cavity implants

Method used

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  • Antibacterial peptide bionic calcium phosphate coating and preparation method and application thereof
  • Antibacterial peptide bionic calcium phosphate coating and preparation method and application thereof

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preparation example Construction

[0026] The preparation method of the above-mentioned antimicrobial peptide biomimetic calcium phosphate coating is specifically implemented according to the following steps:

[0027] Step 1, preparation of one solution of biomimetic calcium phosphate coating:

[0028] First, slowly add 650-750mM NaCl; 15-25mM NaHCO to deionized water in order 3 ; 2~7mMNa 2 HPO 4 2H 2 O; 5 ~ 10mM MgCl 2 2H 2 O, then slowly add HCl (the mass fraction of HCl is 36%~38%) and trishydroxymethylaminomethane (TRIS base) in the solution to jointly adjust the pH of the solution, the pH value of the solution is adjusted to 2~2.5, and then slowly Add 10~15mM CaCl 2 2H 2 O, finally add TRIS base to adjust the pH value of the solution to 6-6.5, and after constant volume, filter the prepared solution in a 0.2 μm filter membrane ultra-clean workbench to obtain the product.

[0029] Step 2, preparation of the second solution of biomimetic calcium phosphate coating:

[0030] Slowly add 1~5mM Na to deio...

Embodiment 1

[0039] Slowly add 650mM NaCl; 15mM NaHCO to deionized water in sequence 3 ; 2mM Na 2 HPO 4 2H 2 O; 5mM MgCl 2 2H 2 O, then slowly add 36% to 38% HCl and TRIS base to the solution to jointly adjust the pH of the solution, slowly adjust the pH value of the solution to 2 through the cooperation of the two, and then slowly add 10mM CaCl 2 2H 2 0. Finally, TRISbase was added to adjust the pH value of the solution to 6. After constant volume, the prepared solution was filtered in a 0.2 μm filter membrane ultra-clean workbench to obtain a solution of biomimetic calcium phosphate coating.

[0040] Slowly add 1 mM Na to deionized water sequentially 2 HPO 4 2H 2 O; 1mM mass fraction of 36% ~ 38% HCl; 3mM CaCl 2 2H 2 O; add TRIS base to adjust the pH value of the solution to 7, and after constant volume, filter the prepared solution in a 0.2 μm filter membrane ultra-clean workbench to obtain the second solution of biomimetic calcium phosphate coating.

[0041] Antimicrobial pe...

Embodiment 2

[0044] Slowly add 680mM NaCl; 18mM NaHCO to deionized water in sequence 3 ; 3mM Na 2 HPO 4 2H 2 O; 6mM MgCl 2 2H 2 O, then slowly add 36% to 38% HCl and TRIS base to the solution to jointly adjust the pH of the solution, slowly adjust the pH value of the solution to 2.2 through the cooperation of the two, and then slowly add 11mM CaCl 2 2H 2 0. Finally, add TRIS base to adjust the pH value of the solution to 6.2. After constant volume, filter the prepared solution in a 0.2 μm filter membrane ultra-clean workbench to obtain a solution of biomimetic calcium phosphate coating.

[0045] Slowly add 2mM Na to deionized water sequentially 2 HPO 4 2H 2 O; 2mM mass fraction of 36% ~ 38% HCl; 4mM CaCl 2 2H 2 O; add TRIS base to adjust the pH value of the solution to 7.2, and after constant volume, filter the prepared solution in a 0.2 μm filter membrane ultra-clean workbench to obtain the second solution of biomimetic calcium phosphate coating.

[0046] Antimicrobial peptide ...

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Abstract

The invention discloses an antibacterial peptide bionic calcium phosphate coating. The antibacterial peptide bionic calcium phosphate coating includes an amorphous calcium phosphate coating body and an antibacterial peptide-containing crystalline calcium phosphate coating body, wherein the amorphous calcium phosphate coating body and the antibacterial peptide-containing crystalline calcium phosphate coating body are sequentially coated on the surface layer of a to-be-coated material from inside to outside. The invention also discloses a preparation method and application of the antibacterial peptide bionic calcium phosphate coating. The antibacterial peptide bionic calcium phosphate coating can deposit under the conditions of the physiological temperature and physiological pH values, the activity of biologically active protein is not destroyed, and the structure of a formed crystal is similar to the bone structure. With formation of the calcium phosphate coating crystal, the antibacterial peptide is incorporated into the network crystal of the coating through co-deposition, and the activity is maintained, and is slowly released under physiological conditions, so that antibacterialproperties are given to the material without influences on attachment, survival and growth and differentiation of cells, and broad application prospects are achieved.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to an antibacterial peptide biomimetic calcium phosphate coating, and also relates to a preparation method of the coating and an application of the antibacterial peptide biomimetic calcium phosphate coating. Background technique [0002] In recent years, with the increasing number of patients treated with oral implants, the incidence of peri-implant diseases has increased rapidly. Peri-implant disease has become an important cause of affecting the effect of implant treatment and leading to implant failure. At present, for patients with severe peri-implantitis, surgery is often performed to remove the inflammatory tissue around the implant and transplant bone substitute materials into the bone defect area. However, it is difficult to completely remove the pathogenic bacteria on the surface of the implant and in the surrounding tissues. The bone substitute materials commonly used in c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/34A61L27/32A61L27/30A61L27/50A61L27/54
CPCA61L27/306A61L27/32A61L27/34A61L27/50A61L27/54A61L2300/252A61L2300/404A61L2300/602A61L2400/18A61L2420/02A61L2420/04A61L2420/06A61L2420/08A61L2430/02A61L2430/12C08L89/00
Inventor 刘宁朱勇刘昌奎郭芳黄硕
Owner XIAN MEDICAL UNIV
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