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Extraction method and application of Hexagona apiaria fungal oil

A technology of cellulite bacteria and extraction method, which is applied in the extraction technology and application field of bacterial oil, can solve the problem of no hairy cellulite bacterial oil, etc., and achieve the effects of easy implementation, simple operation, and various types

Pending Publication Date: 2020-01-17
FOSHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far there are no relevant reports and related products about the hair cellulite bacterial oil

Method used

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  • Extraction method and application of Hexagona apiaria fungal oil
  • Extraction method and application of Hexagona apiaria fungal oil
  • Extraction method and application of Hexagona apiaria fungal oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The preparation of embodiment 1 hair cellulum bacterium oil

[0035] Material: Hexagonia apiaria (Pers.) Fr. was collected from dry longan branches in Nandan Mountain Scenic Area, Sanshui District, Foshan City, Guangdong Province on October 27, 2018.

[0036] Diethyl ether: analytically pure;

[0037] n-Hexane: analytically pure;

[0038] Ethyl acetate: analytically pure.

[0039] Take the above-mentioned wild hair cellulite after natural air-drying, pulverize, and extract with diethyl ether at 50°C for 24 hours at a material-to-liquid ratio of 1:20. Oil;

[0040] The crude bacterial oil was purified by silica gel column chromatography, eluted with n-hexane-ethyl acetate with a volume ratio of 9:1, the eluate was collected, and the solvent was recovered by vacuum rotary distillation at 50°C to obtain the hair cellulite bacterial oil .

[0041] The oil yield of the above-mentioned extracted Trichocellus bacterium oil is 0.26%.

[0042] The inventor has also tried t...

Embodiment 2

[0043] The analysis of the chemical constituents of embodiment 2 hair cellulum bacterium oil

[0044] 2.1. Instruments and materials used

[0045] In embodiment 1, diethyl ether extracts the purified hair cellulite bacterium oil;

[0046] Agilent 7890A gas chromatograph coupled with Agilent 5975C mass spectrometer.

[0047] 2.2. Analytical conditions and methods

[0048] 1) Chromatographic conditions: HP-5MS quartz capillary column (30m×250μm×0.25μm) is used as the chromatographic column; the temperature is raised by program: the initial temperature is 40°C, the temperature is raised to 150°C at a speed of 5°C / min, and then the temperature is increased to 10°C / min The speed was raised to 280°C, and the temperature was kept constant for 10 minutes; the temperature of the injection port was 280°C, and the injection volume was 1 μL; the split ratio was 20:1; the carrier gas was helium.

[0049] 2) Mass spectrometry conditions: EI ion source, ion source temperature 280°C, elect...

Embodiment 3

[0057] Example 3 Evaluation of Antioxidant Activity of Hairy Cellular Bacteria Oil

[0058] 3.1 Materials

[0059] Reagents: 1,1-diphenyl-2-picrylhydrazyl (DPPH), ascorbic acid, absolute ethanol;

[0060] Sample: the hair cellulite bacterium oil prepared by the method described in embodiment 1;

[0061] 3.2 Experiment of scavenging DPPH free radicals with the oil of Trichomonas

[0062] A microplate reader was used to measure the in vitro scavenging ability of DPPH free radicals of different concentrations of sample solutions. Use a pipette gun to accurately pipette 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, and 50 μL of sample solution (initial concentration: 10 mg / mL) into the 96-well microtiter plate, and then add no Dilute with water and ethanol so that the total volume of each well is 50 μL, then use a pipette to accurately absorb 100 μL of the prepared DPPH solution (0.2 mmol / L) into the sample solution, and finally add absolute ethanol to make the total volume of each wel...

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Abstract

The invention discloses an extraction method and application of Hexagona apiaria fungal oil. The extraction method comprises the following steps: weighing the sporocarp of Hexagona apiaria, carrying out drying and crushing, adding a solvent, carrying out continuous reflux percolation extraction at 50 DEG C, collecting an extract, and performing water bath distillation at 50 DEG C for recovery of the solvent so as to obtain crude fungal oil; and subjecting the crude fungal oil to an adsorption chromatographic column, carrying out eluting with an eluent, collecting eluate, and carrying out vacuum rotary distillation at 50 DEG C to recover the solvent so as to obtain the Hexagona apiaria fungal oil. The method is simple to operate and high in oil yield and does not destroy the structures of the effective components of Hexagona apiaria, and the solvent can be recycled. The Hexagona apiaria fungal oil has excellent oxidation resistance, is free of toxicity and harm and rich in source, and can be used for preparing antioxidant health-care products.

Description

technical field [0001] The invention relates to the extraction technology and application field of bacterial oil, in particular to an extraction method and application of hair cellulite bacterial oil. Background technique [0002] Hair cell fungus, Latin scientific name Hexagonia apiaria (Pers.) Fr., also known as longan comb, is a kind of fungus belonging to Basidiomycota, Aphyllophorales, Polyporaceae, honeycomb fungus Rare medicinal fungi of the genus Hexagona. Widely distributed in tropical and subtropical regions, it mainly grows on the trunks of broad-leaved trees. According to the records of "Chinese Herbal Medicine", the hair cellulite is slightly bitter, astringent, and slightly warm, and has the effects of invigorating the intestines, regulating qi, relieving pain, and invigorating the stomach. Li Haohua and others found that the ethyl acetate extract of the fermentation broth of Hairy cellulosus can significantly inhibit the activity of lung cancer cell NCI-H460...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/04C11B1/10C11B3/12A23L33/115
CPCC11B1/04C11B1/10C11B3/12A23L33/115
Inventor 李皓姚欣朱峰
Owner FOSHAN UNIVERSITY
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