A manganese dioxide/glucose oxidase@hyaluronic acid composite anticancer material and its preparation and application
A technology of glucose oxidase and manganese dioxide, applied in the fields of nanotechnology and cancer treatment, can solve the problems of insufficient selectivity and low anti-cancer activity, and achieve good selectivity, low toxicity and side effects, good basic research value and application prospects Effect
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Embodiment 1
[0045] Synthesis and Characterization of TSEN Nanomedicine in Example 1
[0046] (1) Take four groups of 500 μL manganese dioxide nanosheet original solutions (concentration of 0.5 mg·mL) -1 ; The above preparation example is prepared; the following experimental cases, unless otherwise stated, are solutions of this concentration) respectively and 10 μL glucose oxidase solution (5 mg·mL -1 , the following experimental cases, unless otherwise stated, are all solutions of this concentration) mixed;
[0047] (2) The above mixture was added to 1.5, 2, 2.5, and 3 mL of hyaluronic acid solution (2 mg·mL), respectively. -1 ), marked as group 1, group 2, group 3, group 4;
[0048] (3) After magnetic stirring at room temperature overnight (the time is about 12h), a suspension is obtained respectively;
[0049] (4) Put the above suspension at 6000r·min -1 Centrifuge at high speed for 20 min and wash with ultrapure water 2 to 3 times;
[0050] (5) The hydrated particle size of the fo...
Embodiment 2
[0055] Solution stability study of TSEN nanomedicine in Example 2
[0056] (1) The mixed system of manganese dioxide nanosheets and glucose oxidase (the ratio is the same as that in step (1) of Example 1) and the TSEN nanosystem (obtained in step (6) of Example 1) were added to deionized water (DI water). ), physiological saline (Saline), phosphate buffered saline (PBS), and cell culture medium (10% FBS Medium) containing 10% fetal bovine serum;
[0057] (2) wherein, the mixed system of manganese dioxide nanosheet and glucose oxidase is set as Control group;
[0058] (3) at room temperature, let stand for 30 days respectively;
[0059] (4) Observe and record the changes of TSEN in each solution.
[0060] Analysis of the results: from figure 2 The solution stability test of TSEN shows that the mixed system of manganese dioxide and glucose oxidase is deposited on the first day of standing in each solution, while TSEN can still be stably dispersed in deionized water after sta...
Embodiment 3
[0061] Study on the performance of TSEN nano-drug synergistically producing OH in Example 3
[0062] Prepare 25mM NaHCO 3 / 5%CO 2 Buffer solution is ready for use.
[0063] (1) Experimental group A: TSEN nanosystem (prepared in step (6) of Example 1), 2 mg·mL -1 ) added to the presence or absence of 120~150U·mL -1 25mM NaHCO for Hyaluronidase 3 / 5%CO 2 Buffer solution (containing 10 mM GSH, 10 mM glucose);
[0064] (2) Experimental group B: Control probe system (composed of manganese dioxide nanosheets and hyaluronic acid), TSEN nanosystem (2 mg·mL) -1 ) were added to 25mM NaHCO 3 / 5%CO 2 Buffer solution (containing 120~150U·mL -1 Hyaluronidase, 10 mM GSH, 10 mM glucose);
[0065] (3) place the above-mentioned A, B two groups of experimental solution systems to shake at 37°C respectively;
[0066] (4) Centrifuge after 4h, take the supernatant respectively;
[0067] (5) Then add 10 μg·mL -1 Methylene blue (MB) and 50 μM H 2 O 2 were respectively added to the supe...
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