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SSR markers closely linked to maize seed storability and its application in molecular marker-assisted breeding

A molecular marker and corn technology, applied in the direction of microbial determination/inspection, DNA/RNA fragments, biochemical equipment and methods, etc., can solve the problem of reducing the vitality of Arabidopsis seeds

Active Publication Date: 2022-05-27
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Li et al. (Li T, Zhang Y, Wang D, et al.Regulation of Seed Vigor by Manipulation of Raffinose Family Oligosaccharides in Maize and Arabidopsisthaliana[J]. Molecular Plant, 2017, 10(12):1540.) are related to seed vigor ZmGOLS2, ZmRS and AtSTS genes were studied, and it was found that the simultaneous overexpression of ZmGOLS2 and ZmRS genes and the overexpression of ZmGOLS2 gene alone increased the seed vigor of Arabidopsis thaliana, while the overexpression of ZmRS gene alone decreased the seed vigor of Arabidopsis thaliana

Method used

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  • SSR markers closely linked to maize seed storability and its application in molecular marker-assisted breeding
  • SSR markers closely linked to maize seed storability and its application in molecular marker-assisted breeding
  • SSR markers closely linked to maize seed storability and its application in molecular marker-assisted breeding

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Example 1 Mapping of QTLs related to corn seed storage tolerance

[0060] 1 Test material

[0061] Taking the storable inbred line Dong 156 and the inbred line Dong 237 not storable as the parents, and self-inbred for 7 generations by the single-seed method, a RIL population containing 288 families was obtained, which was used for QTL analysis of seed storability and storability. Tag development. Among them, Dong 156 has strong storability and hard grain type, and the germination rate remains above 90% under natural storage conditions for 8 years; Dong 237 has poor storability, and the grain is half-dentate, and the germination rate is stored for 1 year under natural conditions. down to around 80%. Both inbred lines were bred and preserved by the Maize Research Institute of Northeast Agricultural University.

[0062] 2 Test methods

[0063] 2.1 Genotype detection

[0064] 2.1.1 Extraction of total DNA from maize leaves

[0065] The total DNA of the leaves of the 3...

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Abstract

The invention discloses a molecular marker closely linked with the main effect QTL segment related to corn seed storage tolerance and its application; the molecular marker is related to the main effect of corn seed storage tolerance located in the Bin10. The QTL segment cQTL‑10 is closely linked, and the molecular markers are phi050 and umc1648. The present invention locates a main effect QTL section of corn seed storage tolerance, and this section contains two QTLs qRVI-10 and qRSVI-10, which affect the relative vigor index and the relative simple vigor index respectively, and find that it is related to the corn seed Two SSR molecular markers, phi050 and umc1648, closely linked for storability, the SSR molecular markers provided by the present invention can be applied to molecular assisted breeding for improving the storability of maize seeds.

Description

technical field [0001] The present invention relates to SSR molecular markers related to maize seed storability, in particular to a molecular marker closely linked to cQTL-10 located in the main QTL segment related to maize seed storability, the present invention further relates to the molecular marker in molecular assisted The application in breeding belongs to the field of molecular marker and its application. Background technique [0002] Seed storability is the ability of seeds to tolerate storage. Different varieties have different storage physiological characteristics due to different genetic basis, which are comprehensively reflected in the needs and adaptability of seeds to the environment before and during storage. Seed vigor refers to the potential germination ability of seeds or the vitality of seed embryos, and it is one of the important characteristics to measure whether seeds are resistant to storage. Seeds with normal physiological activity under dry conditio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156C12Q2600/13
Inventor 邸宏张林王振华封陈晨周羽曾兴
Owner NORTHEAST AGRICULTURAL UNIVERSITY